2013 Fiscal Year Final Research Report
Role of GPR103-26RFa-QRFP system in the development of neuropathic pain
| Project/Area Number |
23590715
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pain science
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Tatsuo 熊本大学, 大学院生命科学研究部, 教授 (20200818)
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| Project Period (FY) |
2011 – 2013
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| Keywords | GPR108 / orphan receptor / 神経障害性疼痛 / 炎症性疼痛 / 可塑性 |
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| Research Abstract |
In the present study, the effects of intrathecal and intracerebroventricular application of 26RFa, on the level of mechanical allodynia induced by partial sciatic nerve ligation, were examined in rats. Intrathecal and intracerebroventricular injection of 26RFa attenuated the level of mechanical allodynia in a dose dependent manner. Immunohistochemical examination revealed that QRFP-like immunoreactivity (QRFP-LI) was expressed mainly in the small to medium sized neuronal profiles in the L5 dorsal root ganglion and that partial sciatic nerve injury increased the percentage of QRFP-LI positive neuronal profiles and made the cross-section area of the QRFP-LI positive neuronal profiles larger in the L5 dorsal root ganglion ipsilateral to the nerve injury.
Microinjection of 5 g of 26RFa into RVM decreased the number of flinching behavior induced by paw formalin injection significantly as compared with saline injected rats. This effect of 26RFa was not antagonized by naloxone.
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Research Products
(3 results)
