2013 Fiscal Year Final Research Report
Pain regulation by PKA and calcineurin through functional modulation of T-type calcium channels
| Project/Area Number |
23590729
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pain science
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| Research Institution | Kinki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SEKIGUCHI Fumiko 近畿大学, 薬学部, 准教授 (90271410)
TSUBOTA Maho 近畿大学, 薬学部, 助教 (90510123)
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| Project Period (FY) |
2011 – 2013
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| Keywords | カルシウムチャネル / ＰＫＡ / カルシニューリン / 痛み / 神経突起伸長 |
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| Research Abstract |
Among three isoforms of T-type calcium channels, Cav3.2 expressed abundantly in the peripheral ending of nociceptors plays a critical role in nociceptive processing. We thus examined molecular mechanisms for functional regulation of Cav3.2 by phosphorylation and dephosphorylation, and its impact on pain signals. Our data show that PKA, activated by stimulation of prostaglandin EP4 receptors in an AKAP150-dependent manner, phosphorylates and functionally upregulates Cav3.2, leading to hyperalgesia, and that calcineurin, a phosphatase, negatively regulates Cav3.2 functions.
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[Journal Article] AKAP-dependent sensitization of Ca_v3.2 channels via the EP_4 receptor/cyclic AMP pathway mediates prostaglandin E_2-induced mechanical hyperalgesia2013
Author(s)
Sekiguchi, F., Aoki, Y., Nakagawa, M., Kanaoka, D., Nishimoto, Y., Tsubota- Matsunami, M., Yamanaka, R., Yoshida, S. and Kawabata, A
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Journal Title
Br. J. Pharmacol
Volume: Vol. 168
Pages: 734-745
DOI
Peer Reviewed
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[Presentation] Molecular mechanisms for the neurite outgrowth caused by prostaglandin E_2 in mouse dorsal root ganglion cells2012
Author(s)
Maeda, T., Mitani, K., Yamagata, R., Sekiguchi, F., Kawabata, A
Organizer
Neuroscience 2012
Place of Presentation
New Orleans, USA
Year and Date
20121013-27
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[Presentation] Roles of endogenous hydrogen sulfide in the cyclic AMP-induced neuronal differentiation in NG108-15 cells2012
Author(s)
Kanaoka, D., Takeda, Y., Sekiguchi, F., Yoshida, S., Kawabata, A
Organizer
Neuroscience 2012
Place of Presentation
New Orleans, USA
Year and Date
20121013-27
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[Presentation] Prostaglandin E_2-induced sensitization of T-type Ca^<2+> channels : a mechanism for mechanical hyperalgesia2012
Author(s)
Kanaoka, D., Sekiguchi, F., Nakagawa, M., Aoki Y., Nishimoto Y., Yoshida, S., Tsubota-Matsunami, M, Kawabata, A
Organizer
A Satellite Symposium of IUPHAR-GI Section, International Ulcer Week 2012
Place of Presentation
東京
Year and Date
2012-07-12
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[Presentation] AKAP-dependent modulation of phosphorylation levels of Ca_v3.2 T-type calcium channels by protein kinase A and calcineurin : implications for mechanical pain sensitivity2011
Author(s)
Sekiguchi, F., Kanaoka, D., Nakagawa, M., Aoki, Y., Nishimoto, Y., Yoshida, S., Matsunami, M., Kawabata, A
Organizer
Neuroscience 2011
Place of Presentation
Washington, DC
Year and Date
20111112-16
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[Presentation] Sensitization of T-type calcium channels by prostaglandin E_2 via the EP4 receptor/cyclic AMP/protein kinase A pathway : a possible mechanism for mechanical hyperalgesia2011
Author(s)
Kanaoka, D., Sekiguchi, F., Nakagawa, M., Aoki, Y., Nishimoto, Y., Yoshida, S., Matsunami, M., Kawabata, A
Organizer
Neuroscience 2011
Place of Presentation
Washington, DC
Year and Date
20111112-16
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