2013 Fiscal Year Final Research Report
Development of rapid and simple diagnostic assays for food poisoning bacteria and viruses using monoclonal antibodies
| Project/Area Number |
23590749
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | University of Hyogo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KATO Yoji 兵庫県立大学, 環境人間学部, 教授 (30305693)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 食中毒 / 単クローン抗体 / 迅速簡便診断法 / ELISA法 / イムノクロマト法 |
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| Research Abstract |
Monoclonal antibodies (MAbs) are powerful tools for the study of microorganism. A panel of MAbs against food poisoning bacteria and viruses would be valuable for antigenic and structural analysis as well as useful for developing new diagnostic assays. The MAbs are of particular interest as possible reagents for the development of rapid and simple diagnostic assays (i.e., ELISA or immunochromatography) in clinical settings. In this study, we tried to establish such a panel of MAbs specific-reactive to food poisoning bacteria and viruses.
We obtained 8 MAbs to each bacterium. With an ELISA, Six MAbs were specific to each bacterium: enterotoxigenic Escherichia coli, Salmonella enterica serovar enteritidis, Vibrio parahaemolyticus, Campylobacter jejuni, Verotoxin producing Escherichia coli or Staphylococcus aureus. Three MAbs were further analyzed with an immunochromatography.
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