Studies on fever with unknown origin (FUO0 in South and South East Asia

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590770
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Public health/Health science
• Research Institution: Hokkaido University
• Principal Investigator: MORIMATSU Kumiko (吉松 組子) 北海道大学, 医学(系)研究科(研究院), 准教授 (90220722)
• Co-Investigator(Renkei-kenkyūsha): ARIKAWA Jiro 北海道大学, 大学院医学研究科, 教授 (10142704) ; SHIMIZU Kenta 北海道大学, 大学院医学研究科, 助教 (20466840)
• Project Period (FY): 2011 – 2013
• Keywords: ハンタウイルス感染症 / レプトスピラ / HEV / 不明熱

Research Abstract

The investigation of hantavirus-associated infectious diseases in Southeast Asia and South Asia had been conducted. Throughout the investigation, many examples of fever of unknown origin (FUO) were found from febrile diseases. In this report, it aimed to clarify FUO in this region. Febrile patient sera (not dengue fever and a flu victim) and wild rodent sera as a career of infectious agents conducted investigation of causative agent of febrile illness in Vietnam and Sri Lanka. Finally, it was suggested that seropositive cases against pathogenic organs, such as hepatitis E virus (HEV), leptospira, and hantaviruses are found from a part of FUO patient. Furthermore, it was shown that rodents in both countries also possessed these agents. Therefore rodents were able to be sources of these zoonotic agents.

Research Products

• [Journal Article] Distinct genetic characteristics of Sri Lankan Rattus and Bandicota (Murinae, Rodentia) inferred from mitochondrial and nuclear markers (2014)
  • Author(s): Yasuda PS, Gamage CD, Koizumi N, Nishio S, Isozumi R, Shimizu K, Koma T, Amada T, Suzuki H, Yoshimatsu K, Arikawa J
  • Journal Title: Genes Genet. Syst Volume: (in press)
  • Peer Reviewed
• [Journal Article] Development of an immunochromatography strip test based on truncated nucleocapsid antigens of three representative hantavir1u3se. sK. oma T, Yoshimatsu K, Taruishi M (2014)
  • Author(s): Amada T, Yoshimatsu K, Koma T, Shimizu K, Gamage CD, Shiokawa K, Nishio S, Ahlm C, Arikawa J
  • Journal Title: Virology Journal Volume: (in press)
  • Peer Reviewed
• [Journal Article] Cross-reactivity of secondary antibodies against African rodents and application for sero-surveillance (2013)
  • Author(s): Nakamura I, Hang'ombe BM, Sawa H, Kobayashi S, Orba Y, Ishii A, Thomas Y, Isozumi R, Yoshimatsu K, Mweene AS, Takada A, Sugimoto C, Arikawa J
  • Journal Title: J Vet Med Sci Volume: 75 Pages: 819-825
  • Peer Reviewed
• [Journal Article] Susceptibility of laboratory rats against genotypes 1, 3, 4, and rat hepatitis E viruses (2013)
  • Author(s): Li TC, Yoshizaki S, Ami Y, Suzaki Y, Yasuda SP, Yoshimatsu K, Arikawa J, Takeda N, Wakita T
  • Journal Title: Vet Microbiol Volume: 163 Pages: 54-61
  • Peer Reviewed
• [Journal Article] Characterization of full genome of rat hepatitis e virus strain from Vietnam (2013)
  • Author(s): Li TC, Ami Y, Suzaki Y, Yasuda SP, Yoshimatsu K, Arikawa J, Takeda N, Takaji W
  • Journal Title: Emerg Infect Dis Volume: 19 Pages: 115-118
  • Peer Reviewed
• [Journal Article] Epidemiology of hantavirus infection in Thousand Islands regency of Jakarta, Indonesia (2013)
  • Author(s): Ibrahim IN, Shimizu K, Yoshimatsu K, Yunianto A, Salwati E, Yasuda SP, Koma T, Endo R, Arikawa J
  • Journal Title: J Vet Med Sci Volume: 75 Pages: 1003-1008
  • Peer Reviewed
• [Journal Article] Rapid, whole blood diagnostic test for detecting anti-hantavirus antibody in rats (2013)
  • Author(s): Amada T, Yoshimatsu K, Yasuda SP, Shimizu K, Koma T, Hayashimoto N, Gamage CD, Nishio S, Takakura A, Arikawa J
  • Journal Title: J Virol Methods Volume: 193 Pages: 42-49
  • Peer Reviewed
• [Journal Article] Bunyavirus and its ecology (2012)
  • Author(s): Yoshimatsu K, Arikawa J
  • Journal Title: Uirusu Volume: 62 Pages: 239-250
• [Journal Article] Application of Truncated Nucleocapsid Protein (N) for Serotyping ELISA of Murinae-Associated Hantavirus Infection in Rats (2012)
  • Author(s): Yasuda SP, Yoshimatsu K, Koma T, Shimizu K, Endo R, Isozumi R, Arikawa J
  • Journal Title: J Vet Med Sci Volume: 74 Pages: 215-219
  • Peer Reviewed
• [Journal Article] The N-terminus of the Montano virus nucleocapsid protein possesses broadly cross-reactive conformation-dependent epitopes conserved in rodent-borne hantaviruses (2012)
  • Author(s): Saasa N, Yoshida H, Shimizu K, Sanchez-Hernandez C, Romero-Almaraz MD, Koma T, Sanada T, Seto T, Yoshii K, Ramos C, Yoshimatsu K, Arikawa J, Takashima I, Kariwa H
  • Journal Title: Virology Volume: 428 Pages: 48-57
  • Peer Reviewed
• [Journal Article] Studies on hantavirus infection in small mammals captured in southern and central highland area of Vietnam (2012)
  • Author(s): Luan VD, Yoshimatsu K, Endo R, Taruishi M, Huong VT, Dat DT, Tien PC, Shimizu K, Koma T, Yasuda SP, Nhi L, Arikawa J
  • Journal Title: J Vet Med Sci Volume: 74 Pages: 1155-1162
  • Peer Reviewed
• [Journal Article] A survey of rodent-borne pathogens carried by wild Rattus spp. in Northern Vietnam (2012)
  • Author(s): Koma T, Yoshimatsu K, Yasuda SP, Li T, Amada T, Shimizu K, Isozumi R, Mai LT, Hoa NT, Nguyen V, Yamashiro T, Hasebe F, Arikawa J
  • Journal Title: Epidemiol Infect Pages: 1-9
  • Peer Reviewed
• [Journal Article] Development of a serotyping enzyme-linked immunosorbent assay system based on recombinant truncated hantavirus nucleocapsid proteins for New World hantavirus infection (2012)
  • Author(s): Koma T, Yoshimatsu K, Taruishi M, Miyashita D, Endo R, Shimizu K, Yasuda SP, Amada T, Seto T, Murata R, Yoshida H, Kariwa H, Takashima I, Arikawa J
  • Journal Title: J Virol Methods Volume: 185 Pages: 74-81
  • Peer Reviewed
• [Journal Article] Characterization of self-assembled virus-like particles of rat hepatitis E virus generated by recombinant baculoviruses (2011)
  • Author(s): Li TC, Yoshimatsu K, Yasuda SP, Arikawa J, Koma T, Kataoka M, Ami Y, Suzaki Y, Mai le TQ, Hoa NT, Yamashiro T, Hasebe F, Takeda N, Wakita T
  • Journal Title: J Gen Virol Volume: 92 Pages: 2830-2837
  • Peer Reviewed
• [Journal Article] Serological evidence of Thailand virus-related hantavirus infection among suspected leptospirosis patients in Kandy, Sri Lanka (2011)
  • Author(s): Gamage CD, Yasuda SP, Nishio S, Kularatne SA, Weerakoon K, Rajapakse J, Nwafor-Okoli C, Lee RB, Obayashi Y, Yoshimatsu K, Arikawa J, Tamashiro H
  • Journal Title: Jpn J Infect Dis Volume: 64 Pages: 72-75
  • Peer Reviewed
• [Presentation] ラットE型肝炎ウイルスは人獣共通感染症の病原体か? (2013)
  • Author(s): 清水健太, 濱口杉大, Cuong Ngo Chi, 李天成, 吉松組子, 津田祥美, 塩川愛絵, 西尾佐奈恵, 吉田レイミント, 有吉紅也, 有川二郎
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 20131110-12
• [Presentation] Application of recombinant LigA and LipL32 to the lateral flow immunoassay for the detection of pathogenic Leptospira spp. specific IgG in rodent sera (2013)
  • Author(s): Shiokawa K, Gamage CD, Koizumi N, Nishio S, Koarashi Y, Tsuda Y, Shimizu K, Yoshimatsu K, Arikawa J
  • Organizer: 8th Scientific Meeting of International Leptospirosis Society
  • Place of Presentation: Centennial Hall Kyushu University School of Medicine, Fukuoka, Japan
  • Year and Date: 20131008-11
• [Presentation] Development of an Immunochromatography Strip Test for Detecting Anti-hantavirus Antibody in Rodent and Human Sera by Using an N-terminal Common Antigenic Site of Hantavirus N protein (2013)
  • Author(s): Arikawa J, Amada T, Yoshimatsu K, Hayashimoto N, Koma T, Shimizu K, Gamage CD, Shiokawa K, Nishio S, Ahlm C, Takakura A
  • Organizer: IX International Conference on HFRS HPS & Hantaviruses
  • Place of Presentation: Beijing International Convention Center, Beijing, CHINA
  • Year and Date: 20130605-07
• [Presentation] Role of Neutrophils in the Induction of Pulmonary Edema during Hantavirus Infection in C.B-17Scid Mice (2013)
  • Author(s): Yoshimatsu K, Koma T, Nagata N, Sato Y, Shimizu K, Amada T, Nishio S, Hasegawa H, Arkawa J
  • Organizer: IX International Conference on HFRS HPS & Hantaviruses
  • Place of Presentation: Beijing International Convention Center, Beijing, CHINA
  • Year and Date: 20130605-07
• [Presentation] イムノクロマト法による抗ハンタウイルス抗体の迅速抗体検出法の開発 (2012)
  • Author(s): 天田貴子, 吉松組子, 安田俊平, 清水健太, 駒貴明, 林元展人, 高倉彰, 有川二郎
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪、大阪市
  • Year and Date: 20121113-15
• [Presentation] ハンタウイルスの持続感染成立機構について (2012)
  • Author(s): 吉松組子, 有川二郎
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪、大阪市 (シンポジウム)
  • Year and Date: 20121113-15
• [Presentation] Rapid and whole blood diagnostic test for detecting anti-hantavirus antibody in human and rodent (2012)
  • Author(s): Amada T, Yoshimatsu K, Yasuda SP, Koma T, Shimizu K, Nishio S, Hayashimoto N, Takakura A, Arikawa J
  • Organizer: The 5th Asian Federation of Laboratory Animal Science Associations Congress
  • Place of Presentation: Bangkok, Thailand (oral)
  • Year and Date: 20121010-12
• [Presentation] Development of immunochromatographic test for the detection of HFRS and HPS Hantavirus antibody in the human and rodent serum (2012)
  • Author(s): Amada T, Hayashimoto N, Yoshimatsu K, Yasuda S, Shimizu K, Koma T, Isozumi R, Takakura A, Arikawa J
  • Organizer: XVIII International Congress for Tropical Medicine and Malaria
  • Place of Presentation: Rio de Janeiro, Brazil (poster)
  • Year and Date: 20120923-27
• [Presentation] The role of neutrophils in the induction of pulmonary edema during hantavirus infection in C.B-17Scid mice (2012)
  • Author(s): Koma T, Yoshimatsu K, Shimizu K, Yasuda SP, Isozumi R, Amada T, Arikawa J
  • Organizer: 31st Annual Meeting of American Society of Virology
  • Place of Presentation: Madison, USA
  • Year and Date: 20120721-25
• [Presentation] Development of Immunochromatographic Rapid Test for the Detection of IgG Antibodies Specific to HFRS and HPS Hantaviruses in Human and Rodent Serum. Mini-symposium on Emerging and Re-emerging Viral Diseases in Asia (2012)
  • Author(s): T akako Amada, Nobuhito Hayashimoto, Kumiko Yoshimatsu, Shumpei Yasuda, Kenta Shimizu, Takaaki Koma, Rie Isozumi, Akira Takakura, Jiro Arikawa
  • Organizer: 46th Joint Working Conference on Viral Diseases, The Japan-United States Cooperative Medical Science Program
  • Place of Presentation: Beppu, Japan
  • Year and Date: 20120619-20
• [Presentation] Persistence of Seoul virus in natural host (Rattus norvegicus) (2012)
  • Author(s): Kumiko Yoshimatsu, Shumpei P. Yasuda, Kenta Shimizu, Takaaki Koma, Takako Amada, Tetsu Yamashiro, Futoshi Hasebe, Nguyen Thuy Hoa, Le Thi Quynh Mai, Jiro Arikawa
  • Organizer: The 9th Japan-China International Conference of Virology
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20120612-13
• [Presentation] Development of immunochromatographic test strips for the detection of HFRS and HPS hantavirus antibody in the human and rodent serum (2012)
  • Author(s): Takako Amada, Kumiko Yoshimatsu, Shumpei P. Yasuda, Takaaki Koma, Kenta Shimizu, Rie Isozumi, Nobuhito Hayashimoto, Akira Takakura, Jiro Arikawa
  • Organizer: The 9th Japan-China International Conference of Virology
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20120612-13
• [Presentation] Antigenicity and infectivity of rat hepatitis E viruses (2012)
  • Author(s): Tian-Cheng Li, Kumiko Yoshimatsu, Shumpei P. Yasuda, Jiro Arikawa, Michiyo Kataoka, Yasushi Ami, Yuriko Suzaki, Koji Ishii, Naokazu Takeda and Takaji Wakita
  • Organizer: The 9th Japan-China International Conference of Virology
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20120612-13
• [Presentation] Epidemiology of rat hepatitis E virus infection in human and rodents in Vietnam (2012)
  • Author(s): Kenta Shimizu, Tian-Cheng Li, Shumpei P Yasuda, Kumiko Yoshimatsu, Takaaki Koma, Futoshi Hasebe, Tetsu Yamashiro, Nguyen Thuy Hoa, Le Thi Quynh Mai, Koya Ariyoshi, Jiro Arikawa
  • Organizer: The 9th Japan-China International Conference of Virology
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20120612-13
• [Presentation] Role of hantavirus nucleocapsid protein in intracellular traffic of glycoproteins (2011)
  • Author(s): Kenta Shimizu, Kumiko Yoshimatsu, Takaaki Koma, Shumpei P Yasuda, Jiro Arikawa
  • Organizer: XV International Congress of Virology
  • Place of Presentation: Sapporo Convention Center, Sapporo
  • Year and Date: 20110911-16
• [Presentation] Development of the method for monitoring cytotoxic t lymphocyte (CTL) responses to hantavirus in laboratory rats (2011)
  • Author(s): Shumpei P Yasuda, Kumiko Yoshimatsu, Rika Endo, Kenta Shimizu, Takaaki Koma, Rie Isozumi, Jiro Arikawa
  • Organizer: XV International Congress of Virology
  • Place of Presentation: Sapporo Convention Center, Sapporo
  • Year and Date: 20110911-16
• [Presentation] Characterization of virus-like particles of rat hepatitis e virus generated by recombinant baculovirus (2011)
  • Author(s): Tiancheng Li, Kumiko Yoshimatsu, Shumpei P Yasuda, Jiro Arikawa, Michiyo Kataoka, Yasushi Ami, Yuriko Suzaki, Takaji Wakita
  • Organizer: XV International Congress of Virology
  • Place of Presentation: Sapporo Convention Center, Sapporo
  • Year and Date: 20110911-16
• [Presentation] Seroprevalence of anti-leptospira antibodies among patients with acute febrile illness with renal dysfunction in spite of negative result with several laboratorial leptospira tests in Thailand (2011)
  • Author(s): Rie Isozumi, Kumiko Yoshimatsu, Sirima Pattamadilok, Sanit Kumperasart, Jiro Arikawa
  • Organizer: XIII International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: Sapporo Convention Center, Sapporo
  • Year and Date: 20110906-10