2013 Fiscal Year Final Research Report

Effect of bleeding rate on renal dysfunction following hemorrhagic shock in rat

Research Project

Project/Area Number	23590865
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Legal medicine
Research Institution	University of Occupational and Environmental Health, Japan
Principal Investigator	SATO Hiroaki 産業医科大学, 医学部, 准教授 (50441845)
Co-Investigator(Kenkyū-buntansha)	TANAKA Toshiko 産業医科大学, 医学部, 講師 (80141745) KASAI Kentaro 産業医科大学, 医学部, 助教 (40169397)
Project Period (FY)	2011 – 2013
Keywords	出血速度 / 出血性ショック
Research Abstract	The effect of bleeding speed on renal dysfunction was studied using non-hemorrhaging group, three groups of rats who were bled up to 25% of total body blood volume for 5, 20 and 60 minutes, and the other one was bled for 60 minutes treated with cytokine inhibitors. The recovery of MBP; secretion of plasma norepinephrine, epinephrine, and aldosterone; the expression of proinflammatory cytokines, such as TNF-a and IL-1b, deteriorated as the bleeding rate increased due to a blunting autoregulatory hemodynamic responses to hemorrhagic shock. The severity of renal dysfunction, manifested by blood urea nitrogen, serum creatinine concentration, arterial blood gas analysis and histological damage in the kidney, were well correlated with the degree of decrease in bleeding rate.The bleeding rate was one of the key factors on hemorrhagic renal dysfunction through the suppression of hemodynamic autoregulatory responses and the expression of proinflammatory cytokines.