Membrane Function and Bone-Metabolism in the Pathophysiology of the Metabolic Syndrome

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590901
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: Kansai University of Health Sciences
• Principal Investigator: TSUDA KAZUSHI 関西医療大学, 保健医療学部, 教授 (90217315)
• Project Period (FY): 2011 – 2013
• Keywords: 高血圧 / メタボリックシンドローム / 細胞膜fluidity / 電子スピン共鳴 / 赤血球 / nitric oxide / 酸化ストレス / 慢性腎臓病

Research Abstract

The purpose of the present study was to examine possible relationship between kidney function and membrane fluidity of red blood cells (RBCs) in hypertensive and normotensive subjects using an electron spin resonance and spin-labeling method. Membrane fluidity was significantly decreased in hypertensive subjects. The decreased eGFR value might be associated with reduced membrane fluidity of RBCs, suggesting that CKD might have a close correlation with microcirculatory disorders. The reduced levels of both membrane fluidity of RBCs and eGFR were associated with increased plasma 8-iso-prostaglandin F2alpha (an index of oxidative stress) and decreased plasma nitric oxide-metabolites. On the other hand, bone mineral density was significantly lower in hypertensive elderly women than in normotensive elderly women. We propose that CKD-related changes in membrane function and bone-metabolism might have a crucial role in the pathophysiology of hypertension and the metabolic syndrome.

Research Products

• [Journal Article] Role of hyperleptinemia in the regulation of cardiac function (2014)
  • Author(s): Tsuda K
  • Journal Title: Hypertension Volume: 63 Pages: e1
• [Journal Article] Sympatho-inhibitory effect of diltiazem and prevention of aneurysm formation (2014)
  • Author(s): Tsuda K
  • Journal Title: Hypertension Volume: 63 Pages: e12
• [Journal Article] Asymmetric dimethylarginine and DDAH transcript variants in cardio- vascular and cerebrovascular diseases (2014)
  • Author(s): Tsuda K
  • Journal Title: Am J Hypertens Volume: 27 Pages: 497
• [Journal Article] Letter regarding article, Serum alkaline phosphatase and phosphate in cerebral atherosclerosis and functional outcomes after cerebral infarction (2014)
  • Author(s): Tsuda K
  • Journal Title: Stroke Volume: 45 Pages: e46
• [Journal Article] Chronic kidney disease predicts impaired membrane microviscosity of red blood cells in hypertensive and normotensive subjects. an electron spin resonance study (2013)
  • Author(s): Tsuda K
  • Journal Title: Int Heart J Volume: 54 Pages: 154-159
• [Journal Article] Angiotensin-(1-7) and bradykinin in baroreceptor reflex sensitivity in hypertension (2013)
  • Author(s): Tsuda K, Nautiyal M, Chappell MC, Diz DI
  • Journal Title: Hypertension Volume: 61 Pages: e19-20
• [Journal Article] Roles of sex steroid hormones and nitric oxide in the regulation of sympathetic nerve activity in women (2013)
  • Author(s): Tsuda K, Carter JR, Fu Q, Minson MT, Joyner MJ
  • Journal Title: Hypertension Volume: 61 Pages: e36-37
• [Journal Article] Low serum adiponectin levels and endothelial dysfunction in childhood hypertension (2013)
  • Author(s): Tsuda K, Brambilla P, Antolini L, Street ME, Giussani M, Galbiati S, Valsecchi MG, Stella A, Zucchotti GV, Bernasconi S, Genovsi S
  • Journal Title: Am J Hypertens Volume: 26 Pages: 717-718
• [Journal Article] Hyperhomocysteinemia and oxidative stress levels are associated with impaired membrane fluidity of red blood cells in hypertensive and normotensive men : an electron spin resonance investigation (2013)
  • Author(s): Tsuda K
  • Journal Title: Int J Clin Med Volume: 4 Pages: 58-65
• [Journal Article] Letter regarding article, Impact of ACE2 deficiency and oxidative stress on cerebrovascular function with aging (2013)
  • Author(s): Tsuda K, Pena-Silva RA, Faraci FM, Heistad DD
  • Journal Title: Stroke Volume: 44 Pages: e34-35
• [Journal Article] Hypoadiponectinemia and endogenous nitric oxide synthase inhibitor in hypertension (2013)
  • Author(s): Tsuda K, Xing W, Ji L, Li Y, Gao F, Yan W, Liu P, Sun L, Tao L, Zhang H
  • Journal Title: Hypertension Volume: 62 Pages: e4-5
• [Journal Article] Letter regarding article, Low plasma arginine : asymmetric dimethylarginine ratios predict mortality after intracranial aneurysm rupture (2013)
  • Author(s): Tsuda K, Staalso JM, Romner B, Olsen NV
  • Journal Title: Stroke Volume: 44 Pages: e92-93
• [Journal Article] Angiotensin-(1-7) in the central nervous system regulation of blood pressure and renin-angiotensin system (2013)
  • Author(s): Tsuda K, Vargic J, Ferrario M
  • Journal Title: Am J Hypertens Volume: 26 Pages: 1174-1175
• [Journal Article] Glutamate receptors and presympathetic neuronal hyperactivity of the central nervous system in hypertension (2013)
  • Author(s): Tsuda K, Ye ZU, Li DP, Pan HL
  • Journal Title: Hypertension Volume: 62 Pages: e33-34
• [Journal Article] Chronic kidney disease and membrane microviscosity in hypertension -an electron spin resonance study- (2013)
  • Author(s): Tsuda K
  • Journal Title: J Jpn Coll Angiol Volume: 53 Pages: 179-183
• [Journal Article] Letter regarding article, Association of Chronic kidney disease with cerebral microbleeds in patients with primary intracerebral hemorrhage (2013)
  • Author(s): Tsuda K, Ovbiagele B, Kidwell CS
  • Journal Title: Stroke Volume: 44 Pages: e231-232
• [Journal Article] Aerobic exercise and circulatory dysfunction in resistant hypertension (2012)
  • Author(s): Tsuda K, Dimeo F, Pagonas N, Westhoff TH
  • Journal Title: Hypertension Volume: 60 Pages: e45-46
• [Journal Article] Associations between high-sensitivity C-reactive protein and membrane fluidity of red blood cells in hypertensive elderly men : an electron spin resonance study (2012)
  • Author(s): Tsuda K
  • Journal Title: Int J Hypertens Volume: 2012 Pages: 292803
  • DOI: 10.1155/2012/292803
• [Journal Article] Renin-angiotensin system and sympathetic neurotransmitter release in the central nervous system of hypertension (2012)
  • Author(s): Tsuda K
  • Journal Title: Int J Hypertens Volume: 2012 Pages: 474870
  • DOI: 10.1155/2012/474870
• [Journal Article] Associations of oxidative stress and inflammation and their role in the regulation of membrane fluidity of red blood cells in hypertensive and normotensive men : an electron spin resonance investigation (2012)
  • Author(s): Tsuda K
  • Journal Title: Adv Biosci Biotech Volume: 3 Pages: 1020-1027
• [Journal Article] Roles of adiponectin and oxidative stress in the regulation of membrane microviscosity of red blood cells in hypertensive men-an electron spin resonance study (2011)
  • Author(s): Tsuda K
  • Journal Title: Journal of Obesity Volume: 2011 Pages: 548140
  • DOI: 10.1155/2011/54814
• [Journal Article] Letter regarding article, Endothelial activation in lacunar stroke subtype (2011)
  • Author(s): Tsuda K, Knottnerus ILH, Staals J, van Oostenbrugge RJ
  • Journal Title: Stroke Volume: 42 Pages: e34-35
• [Journal Article] Letter regarding article, Vitamin D deficiency is associated with subclinical carotid atherosclerosis : the Northern Manhattan study (2011)
  • Author(s): Tsuda K, Walker MD, Silverberg SJ
  • Journal Title: Stroke Volume: 42 Pages: e639-640
• [Journal Article] Letter regarding article, Receptor activity-modifying protein-1 augments cerebrovascular responses to calcitonin gene-related peptide and inhibits angiotensin II-induced vascular dysfunction (2011)
  • Author(s): Tsuda K, Chrissobolis S, Russo AF, Faraci FM
  • Journal Title: Stroke Volume: 42 Pages: e24-25
• [Presentation] 細胞膜機能からみた高血圧の血管内皮障害とAdipokineによる調節機構-電子スピン共鳴法を用いた検討- (2013)
  • Author(s): 津田和志
  • Organizer: 第54回日本脈管学会総会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 20131000
• [Presentation] Resistin predicts impaired membrane microviscosity of red blood cells and microcirculatory dysfunction in hypertensive men : an electron spin resonance study (2013)
  • Author(s): Tsuda K
  • Organizer: Featured Research Sessions at the 77th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20130300
• [Presentation] Cystatin C predicts membrane microviscosity of red blood cells and microcirculatory dysfunction in hypertension -an electron spin resonance study (2013)
  • Author(s): Tsuda K
  • Organizer: The 77th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20130300
• [Presentation] 電子スピン共鳴からみた高血圧の細胞膜Microviscosityと腎動脈硬化-特にCKD関連動脈硬化と酸化ストレスとの関連から- (2012)
  • Author(s): 津田和志
  • Organizer: 第53回日本脈管学会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 20121000
• [Presentation] Chronic kidney disease with albuminuria is associated with impaired membrane microviscosity in hypertensive subjects : an electron spin resonance study (2012)
  • Author(s): Tsuda K
  • Organizer: The 35th Annual Scientific Meeting of the Japanese Society of Hypertension (International Session)
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 20120900
• [Presentation] Role of hypercholesterolemia in the regulation of membrane microviscosity of red blood cells in hypertensive and normotensive men : an electron spin resonance study (2012)
  • Author(s): Tsuda K
  • Organizer: Asian Pacific Stroke Conference 2012
  • Place of Presentation: Tokyo
  • Year and Date: 20120900
• [Presentation] Adiponectin and oxidative stress in the regulation of membrane fluidity of red blood cells in hypertensive and normotensive men : an electron spin resonance study (2012)
  • Author(s): Tsuda K
  • Organizer: Asian Pacific Stroke Conference 2012
  • Place of Presentation: Tokyo
  • Year and Date: 20120900
• [Presentation] Electron spin resonance study on the modulatory effect of benidipine, a Ca-channel blocker, on membrane microviscosity of erythrocytes in hypertensive subjects (2012)
  • Author(s): Tsuda K
  • Organizer: Asian Pacific Stroke Conference 2012
  • Place of Presentation: Tokyo
  • Year and Date: 20120900
• [Presentation] Oxidative stress, hyperhomocyteinemia, endothelial dysfunction and membrane microviscosity of red blood cells in hypertensive men -an electron spin resonance study (2012)
  • Author(s): Tsuda K
  • Organizer: The 76th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 20120300
• [Presentation] Chronic kidney disease predicts impaired membrane microviscosity of red blood cells and microcirculatory disorders in hypertension : an electron spin resonance study (2012)
  • Author(s): Tsuda K
  • Organizer: The 76th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 20120300
• [Presentation] Electron spin resonance study on modulatory effects of adiponectin and oxidative stress on membrane microviscosity of erythrocytes in hypertensive men (2011)
  • Author(s): Tsuda K
  • Organizer: The 34th Annual Scientific Meeting of the Japanese Society of Hypertension
  • Place of Presentation: Utsunomiya, Japan
  • Year and Date: 20111000
• [Presentation] Electron spin resonance study on modulatory effects of a Ca-channel blocker, benidipine, on membrane microviscosity of erythrocytes in hypertensive subjects (2011)
  • Author(s): Tsuda K
  • Organizer: The 74th Annual Scientific Meeting of the Japanese Society of Nephrology
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20110600
• [Presentation] Resistin is associated with chronic kidney disease (CKD) and microcirculatory dysfunction in hypertensive subjects
  • Author(s): Tsuda K
  • Organizer: The 36th Annual Scientific Meeting of the Japanese Societyof Hypertension
  • Place of Presentation: Osaka, Japan
  • Year and Date: 00131000
• [Book] Bone mineral density in men and women with essential hypertension. In'Hypertension and Bone Loss', edited by Afrooz Afghani (2011)
  • Author(s): Tsuda K
  • Total Pages: 15-28
  • Publisher: Nova Science Publishers, Inc.