2013 Fiscal Year Final Research Report
To determine a disease pathway related with NKX2.3 in inflammatory bowel disease
| Project/Area Number |
23590932
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAKUTA Yoichi 東北大学, 大学病院, 助教 (50509205)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 炎症性腸疾患 / NKX2.3 / クローン病 / 潰瘍性大腸炎 / 感受性遺伝子 |
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| Research Abstract |
NKX2.3 is expressed in endothelial cells and is considered to be one of the candidate susceptibility genes to inflammatory bowel diseases. In this study, to investigate how NKX2.3 influences disease susceptibilities to inflammatory bowel diseases, we determined the genes regulated by NKX2.3 in endothelial cells using expression analysis and ChIP on Chip. A total of 34 genes including MAdCAM-1 were determined in these analyses. Enriched GO terms were revealed to be inflammatory response and cell proliferation using DAVID. To determine the binding site in the promoter region of the MAdCAM-1, the promoter assay using pGL4 plasmid and electrophoretic mobility shift assay were performed. But we have not found the binding site in the promoter region of the MAdCAM-1.
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