2013 Fiscal Year Final Research Report
Analysis of the role of HBx in the HBV-related hepatocarninogenesis
| Project/Area Number |
23590964
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | B型肝炎ウイルス / 組み込み / X遺伝子 |
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| Research Abstract |
First we analyzed the integration of HBx gene into the chromosome of PLC/PRF/5 cells using fluorescence in situ hybridization, which disclosed the integration of HBx into plural chromosomes. For further analysis, we determined the integrated HBV sequences with the adjacent host genes using next generation sequencer, which disclosed that HBV was integrated into 11 chromosomes. On chromosome 5, HBx gene was integrated into the promoter region of hTERT. The methylation of CpG island in the HBV and adjacent host DNAs were low, which suggested that methylation may play some role in the HBV-associated hepatocarcinogenesis.
Next we analyzed HBV-HCC samples obtained on hepatectomy. HBV was integrated into multiple host DNA, including SLC6A13 and FN1.
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[Journal Article] A new detection method for integration of hepatitis B virus DNA in PLC/PRF/5 Cells2012
Author(s)
Hiraishi T, Watanabe Y, Oikawa R, Hara M, Kiyokawa H, Yoshida Y, Yamada N, Okuse C, Suzuki M, Yotsuyanagi H, Itoh F
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Journal Title
St. Marianna Universirty Journal
Volume: 3
Pages: 63-72
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[Journal Article]
Author(s)
Watanabe Y, Yamamoto H, Oikawa R, Toyoda M, Arii S, Yotsuyanagi H, Itoh F, Koike K
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