2013 Fiscal Year Final Research Report

The role of cell cycle regulator Cdh1 in carcinogenesis and development of gastrointestinal cancer.

Research Project

Project/Area Number	23590980
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Gastroenterology
Research Institution	Kumamoto University
Principal Investigator	NAOE Hideaki 熊本大学, 医学部附属病院, 助教 (30599246)
Co-Investigator(Kenkyū-buntansha)	SASAKI Yutaka 熊本大学, 大学院生命科学研究部, 教授 (70235282) YOKOMINE Kazunori 熊本大学, 医学部付属病院, 助教 (60530128) FUJIMOTO Jirou 兵庫医科大学, 医学部, 教授 (90199373)
Co-Investigator(Renkei-kenkyūsha)	SAYA Hideyuki 慶應義塾大学, 医学部, 教授 (80264282)
Project Period (FY)	2011 – 2013
Keywords	肝臓学 / pathway解析
Research Abstract	Suppress the expression of Cdh1 with siRNA tequnique in colon cancer cell line. These cells revealed cytoskeletal abnormality with decreased actin filament.In the analysis of cell motility with the wound healing assay, Cdh1 suppresse cells showed high motility compared with the control cells. Next, we generated heterogenous mice which one of the phosphorylation site of Cdh1 was suppressed with gene trap tequnique.Genotyping of offspring of these mice was following as wild type: hetero: homo=13:32:0. These results indicated that homo suppression of Cdh1 phosphorylation site was embryonic lethal event in mice.Now,we are studying about the timing of lethality and cause of death.