2013 Fiscal Year Final Research Report
Cyclopedic investigation of genes for NASH-mediated carcinogenesis
| Project/Area Number |
23590996
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Aichi Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAO Haruhisa 愛知医科大学, 医学部, 教授 (60326139)
NAKADE Haruhisa 愛知医科大学, 医学部, 講師 (70431400)
SHIBASUN Daran Karunan 愛知医科大学, 医学部, 助教 (30557096)
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| Project Period (FY) |
2011 – 2013
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| Keywords | NASH / 肝発癌 / オリゴCGHアレイ / コリン欠乏食 |
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| Research Abstract |
We examined the genes expressions for NASH-related hepatocarcinogenesis induced by choline-deficient L-amino acid-defined (CDAA) diet, using origo-CGH microarray analysis. Forty-eight week CDAA diet iuduced 52 genes expression differences in the liver between CDAA diets and control. These included tumor proliferation-related genes, and HNF1/TCF1, transcriptional regulation genes expressions were chronologically attenuated. NF1 which is known to be related signal transaction, are chronologically decreased. Real time PCR showed that HNF1/TCF1 has a tendency to be decreased by CDAA diet, however, NF1 mRNA expressions in the liver were not changed by CDAA diet. These results produced the gene expression differences between origo-CGH microarray analysis and real time PCR. Further investigations were needed to examine in the future.
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