2013 Fiscal Year Final Research Report
Development of transplantation of hepatocytes produced from iPS cells to liver failure
| Project/Area Number |
23591002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | 独立行政法人国立病院機構 下志津病院(臨床研究部) |
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Principal Investigator |
TOMIZAWA Minoru 独立行政法人国立病院機構下志津病院(臨床研究部), その他部局等, その他 (90334193)
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| Co-Investigator(Renkei-kenkyūsha) |
OHIRA Miki 千葉県がんセンター(研究所), がんゲノムセ ンター・がんゲノム研究室, 室長 (20311384)
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| Project Period (FY) |
2011 – 2013
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| Keywords | iPS cells / 肝細胞 / 転写因子 / 増殖因子 / 糖新生 / 尿素サイクル |
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| Research Abstract |
A medium with Activin A was developed for feeder-free culture of iPS cells. iPS cells differentiated to hepatoblasts from iPS cells transfected with C/EBP alpha, GATA4, HEX, and FoxA2 in a medium with Oncostatin M, EGF, and RA. Hepatocyte selection medium was developed without glucose or arginine, supplemented with galactose and ornithine. When HSM was added to co-culture of iPS cells and human primary hepatocytes, iPS cells died and hepatocytes survived. Hepatocytes were successfully purified.
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Research Products
(22 results)
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[Journal Article] Activin A maintains pluripotency markers and proliferative potential of human induced pluripotent stem cells2011
Author(s)
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., and Yoshida, T.
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Journal Title
Exp Therap Med
Volume: 2
Pages: 405-408
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[Presentation] Induced pluripotent stem cells maintained pluripotency markers with Activin A in feeder free culture
Author(s)
Tomizawa, M., Shinozaki, F,. Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T.
Organizer
10th Annual Meeting of International Society of Stem Cell Research (ISSCR)
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