Rho-kinase activity in circulating neutrophils as a useful biomarker for diagnosis and disease activity assessment of vasospastic angina

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591026
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: National Cardiovascular Center Research Institute (2012-2013); Tohoku University (2011)
• Principal Investigator: YASUDA Satoshi 独立行政法人国立循環器病研究センター, 病院, 部門長 (00431578)
• Co-Investigator(Kenkyū-buntansha): SHIMOKAWA Hiroaki 東北大学, 大学院医学系研究科, 教授 (00235681) ; TAKAHASHI Jun 東北大学, 大学院医学系研究科, 講師 (00375081)
• Project Period (FY): 2011 – 2013
• Keywords: ストレス

Research Abstract

We examined whether Rho-kinase activity is systemically enhanced in vasospastic angina (VSA) patients. The Rho-kinase activity was significantly higher in the VSA group than in the non-VSA group. We also found that Rho-kinase activity of VSA patients was transiently enhanced associated with disaster-related mental stress and that Rho-kinase activity exhibits distinct circadian variation associated with alterations in coronary vasomotor responses. These results indicate that Rho-kinase activity in circulating neutrophils may be a useful biomarker for diagnosis and disease activity assessment of the vasospastic disorder.

Research Products

• [Journal Article] Circadian Variation of Rho-Kinase Activity in Circulating Leukocytes of Patients With Vasospastic Angina (2014)
  • Author(s): Nihei T, Takahashi J, Tsuburaya R, Ito Y, Shiroto T, Hao K, Takagi Y, Matsumoto Y, Nakayama M, Miyata S, Sakata Y, Ito K, Shimokawa H.
  • Journal Title: Circ J Volume: Mar 27(Epub ahead of print)
• [Journal Article] Contemporary crossing techniques for infrapopliteal chronic total occlusions (2014)
  • Author(s): Kawarada O, Sakamoto S, Harada K, Ishihara M, Yasuda S, Ogawa H.
  • Journal Title: J Endovasc Ther Volume: 21 Pages: 266-80
• [Journal Article] Vasospastic limb ischemia presenting acute and chronic limb ischemia (2014)
  • Author(s): Kaneyama J, Kawarada O, Sakamoto S, Harada K, Ishihara M, Yasuda S, Hisao O.
  • Journal Title: Ann Vasc Dis Volume: (in press)
• [Journal Article] Enhanced Rho-kinase activity in patients with vasospastic angina after the Great East Japan Earthquake (2012)
  • Author(s): Nihei T, Takahashi J, Kikuchi Y, Takagi Y, Hao K, Tsuburaya R, Shiroto T, Ito Y, Matsumoto Y, Nakayama M, Ito K, Yasuda S, Shimokawa H.
  • Journal Title: Circ J Volume: 76 Pages: 2892-4
• [Journal Article] Enhanced Rho-kinase activity in circulating neutrophils of patients with vasospastic angina : a possible biomarker for diagnosis and disease activity assessment (2011)
  • Author(s): Kikuchi Y, Yasuda S, Aizawa K, Tsuburaya R, Ito Y, Takeda M, Nakayama M, Ito K, Takahashi J, Shimokawa H.
  • Journal Title: J Am Coll Cardiol Volume: 58 Pages: 1231-7
• [Presentation] Circadian Variation of Rho-kinase Activity in Circulating Leukocytes in Patients with Vasospastic Angina Circulation Journal 2014, 78(Suppl. I) : I-597 (2014)
  • Author(s): Taro Nihei, Jun Takahashi, Yoku Kikuchi, Kiyotaka Hao, Yusuke Takagi, Ryuji Tsuburaya, Takashi Shirato, Yoshitaka Itoh, Yasuharu Matsumoto, Masaharu Nakayama, Kenta Ito, Hiroaki Shimokawa
  • Organizer: 第78回日本循環器学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20140321-23
• [Presentation] Circadian variation of Rho-kinase activity associated with enhanced coronary reactivity in patients with vasospastic angina (2013)
  • Author(s): Nihei T, Takahashi J, Kikuchi Y, Tsuburaya R, Shiroto T, Ito Y, Matsumoto Y, Nakayama Y, Ito K, Shimokawa H.
  • Organizer: European Society of Cardiology 2013
  • Place of Presentation: Amsterdam, Netherlands
  • Year and Date: 20130831-0904
• [Presentation] Enhanced rho-kinase activity in circulating neutrophils of patients with vasospasticangina after the Great East Japan Earthquake. (#9574) (2012)
  • Author(s): Nihei T, Takahashi J, Kikuchi Y, Hao K, Takagi Y, Tsuburaya R, Shiroto T, ItoY, Matsumoto Y, Nakayama M, Ito K, Yasuda S, Shimokawa H.
  • Organizer: American Heart Association (AHA) Scientific Sessions
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 20121103-07
• [Presentation] Increased Rho-kinase activity in patients with vasospastic angina after the Great East Japan Earthquake disaster (2012)
  • Author(s): Nihei T, Takahashi J, Kikuchi Y, Ito Y, Tsuburaya R, Takagi Y, Nakayama M, Ito K, Yasuda S, Shimokawa H.
  • Organizer: European Society of Cardiology Congress 2012
  • Place of Presentation: Munich, Germany
  • Year and Date: 20120825-29
• [Book] The Pathogenesis of Vasospastic Angina. In : Kaski JC, Eslick GD, Bairey Merz CN (eds.), Chest Pain with Normal Coronary Arteries (2013)
  • Author(s): Yasuda S, Shimokawa H.
  • Total Pages: 91-99
  • Publisher: Springer-Verlag