2013 Fiscal Year Final Research Report
Induction of reverse remodeling via reconstruction of action potential by transplantation of Kv1.3 transfected fibloblast in failured heart.
| Project/Area Number |
23591061
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Circulatory organs internal medicine
|
|---|
| Research Institution | Kitasato University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NIWANO Hiroe 玉川大学, 教育学部, 教授 (00293233)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | Kv1.3 / Kir2.1 / 電気的リモデリング / 構造的リモデリング / 心不全 / 不整脈 |
|---|
| Research Abstract |
The down-regulation of Ito channel in failure heart will prolong the action potential duration and it accelerates the calcium over-load induced myocardial injury. This study aimed to induce reverse remodeling by removing this vicious circle via normalization of action potential. In our preceding study, we tried to transfect Ito channel directly to the myocyte by using Kv4.2, Kv4.3 and KChIP2 transfection, but enough amount of expression of these molecules myocyte could not be achieved, then we utilized fibroblast transplantation method in this study. We transfected Kv1.3+Kir2.1 into the fibroblast, then tried to transplant them into cardiac tissue. They will construct electrical connection to the myocytes and the action potential will be shortened as well as decrease in calcium over-load in the failure myocytes. In the recent protocol, we succeeded to transfect Kv1.3 to the fibroblast but the stable expression of Kir2.1 was not achieved during the scheduled study period.
|
