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2013 Fiscal Year Annual Research Report

致死性不整脈発生基質としての介在板リモデリングの検討

Research Project

Project/Area Number 23591081
Research InstitutionKyushu University

Principal Investigator

大草 知子  九州大学, 大学病院, 研究員 (00294629)

Keywordsintercalated disk / gap junction / adhesion junction / arrhythmia
Research Abstract

The intercellular junctions contain two complexes, adhesion junction (AJ) and connexin (Cx) gap junction (GJ). GJs provide the pathway for intercellular current flow. AJ mediates normal mechanical coupling and plays an important role in the stability of GJs. We investigated the effects of rapid electrical stimulation (RES) on cardiac intercellular junctions, especially β-catenin and Cx43 alterations. We studied the effects of Angiotensin II receptor blockade on intercellular junctions remodeling. Neonatal rat cardiomyocytes were cultured and subjected to RES. Conduction properties were examined by extracellular potential mapping system. Cx43 protein expression in cardiomyocytes was significantly increased after 60 min. Beta-catenin expression in total cell fraction was significantly increased after 30 min. The expression level of β-catenin in nucleus, which functions as T cell factor/lymphocyte enhancer binding factor (TCF/LEF) transcriptional activator of Cx43 and its degradation is regulated by glycogen synthase kinase 3β (GSK3β), was dramatically increased after 10 min. Conduction velocity was increased significantly by RES for 60min. Olmesartan prevented most these effects of RES. We showed an increase of phosphorylated GSK3β, which is phosphorylated by activated mitogen-activated protein kinases (MAPKs) and inhibits β-catenin degradations, was attenuated by olmesartan. The changes in β-catenin precede Cx43 GJ remodeling. Olmesartan might be a new upstream arrhythmia therapy by modulating intercellular junctions remodeling through β-catenin signaling pathway.

  • Research Products

    (8 results)

All 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (7 results)

  • [Journal Article] Rapid electrical stimulation causes alterations in cardiac intercellular junction proteins of cardiomyocytes2014

    • Author(s)
      Tadamitsu Nakashima, Tomoko Ohkusa, Yoko Okamoto, Masaaki Yoshida, Jong-Kook Lee, Yoichi Mizukami, Masafumi Yano
    • Journal Title

      American Journal of Physiology Heart Circ. Physiol.

      Volume: 306 Pages: in press

    • DOI

      10.1152/ajpheart.00653 2013.

    • Peer Reviewed
  • [Presentation] Alterations in cardiac beta-catenin precede connexin gap junction remodeling in cardiomyocytes exposed to rapid electrical stimulation

    • Author(s)
      Tadamitsu Nakashima, Tomoko Ohkusa, Masaaki Yoshida, J.K.Lee, Masafumi Yano
    • Organizer
      European Society of Cardiololgy Congress 2013
    • Place of Presentation
      Amsterdam
  • [Presentation] アンジオテンシン受容体拮抗薬は不整脈発生基盤となる介在板リモデリングを抑制する

    • Author(s)
      中島唯光,大草知子,吉田雅昭,李 鐘國,水上洋一,矢野雅文
    • Organizer
      第61回日本心臓病学会学術集会
    • Place of Presentation
      熊本
  • [Presentation] 心不全患者における睡眠呼吸障害の有病率に関するコホート研究(HYPNOS)の中間報告

    • Author(s)
      中島唯光,大草知子,原田典子,名尾朋子,和田靖明,上山 剛,小林茂樹,池田安宏,矢野雅文
    • Organizer
      第103回日本循環器学会中国地方会
    • Place of Presentation
      米子
  • [Presentation] 「Cardiac β-catenin Alterations Play an Important Role in Gap Junction Remodeling of Cardiomyocytes Exposed to Rapid Electrical Stimulation」

    • Author(s)
      Tadamitsu Nakashima, Tomoko Ohkusa, Masaaki Yoshida, Jong-Kook Lee, Yoichi Mizukami, Masafumi Yano
    • Organizer
      The 17th Annual Scientific Meeting of the Japanese Heart Failure Society,
    • Place of Presentation
      Saitama
  • [Presentation] 「Wnt/β-catenin Pathway Plays an Important Role in Cx43-dependent Arrhythmogenesis in Cardiomyocytes Exposed Rapid Electrical Stimulation」

    • Author(s)
      Tadamitsu Nakashima, Tomoko Ohkusa, Jong-kook Lee, Masafumi Yano
    • Organizer
      The 78th Annual scientific Meeting of the Japanese Circulation Society,
    • Place of Presentation
      Tokyo
  • [Presentation] 介在板リモデリングへのアンジオテンシン受容体阻害薬の検討

    • Author(s)
      中島唯光、大草知子、李鍾國、水上洋一、矢野雅文
    • Organizer
      第30回日本心電学会学術集会
    • Place of Presentation
      青森
  • [Presentation] コネキシン発現異常と不整脈

    • Author(s)
      本荘晴朗,大草知子,児玉逸雄,神谷香一郎
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      鹿児島

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Published: 2015-05-28  

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