Analysis of the function of CCL1 during respiratory tract infection in C-C motif ligand-1 transgenic mouse

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591111
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Yamagata University
• Principal Investigator: INOUE Sumito 山形大学, 医学部, 助教 (70466621)
• Project Period (FY): 2011 – 2013
• Keywords: 呼吸器 / 感染症 / 慢性閉塞性肺疾患 / 遺伝子改変マウス

Research Abstract

We generated the CCL1 (NCBI: rs2282691) transgenic mice using surfactant protein C promoter (SPC-CCL1 Tg), which overexpressed CCL1 gene in the lungs. We developed the Mycobacterium bovis Bacillus Calmette Guérin (BCG) infection models in SPC-CCL1 Tg mice.
Microscopic observation revealed that the numbers of granulomas in the lungs were significantly increased in SPC-CCL1 Tg mice. In DNA microarray analyses, 4,569 genes had 3 times and higher or one third and lower expression in the lung of Tg mice compare to that of WT mouse. Hierarchical cluster analysis of those genes revealed that Ern1, that plays important roles in endoplasmic reticulum stress and in granuloma formation, was more upregulated in the lung of BCG-treated SPC-CCL1 Tg mice compared to that of wild type mice.CCL1 overexpression increased the formation of granulomas upon BCG infection in the lungs. SPC-CCL1 Tg mice showed significant gene expression of Ern1 in comparison with wild type mice.

Research Products

• [Journal Article] Relationship between Serum Level of Lymphatic Vessel Endothelial Hyaluronan Receptor-1 and Prognosis in Patients with Lung Cancer (2014)
  • Author(s): Nunomiya K, Shibata Y, Abe S, Inoue S, Igarashi A, Yamauchi K, Kimura T, Aida Y, Nemoto T, Sato M, Kishi H, Nakano H, Sato K, Kubota I
  • Journal Title: J Cancer Volume: 5(3) Pages: 242-247
  • Peer Reviewed
• [Journal Article] A lower level of forced expiratory volume in 1 second is a risk factor for all-cause and cardiovascular mortality in a Japanese population : the Takahata study (2013)
  • Author(s): Shibata Y, Inoue S, Igarashi A, Yamauchi K, Abe S, Aida Y, Nunomiya K, Sato M, Nakano H, Sato K, Nemoto T, Kimura T, Watanabe T, Konta T, Daimon M, Ueno Y, Kato T, Kayama T, Kubota I
  • Journal Title: PLoS One Volume: 8(12) Pages: e83725
  • Peer Reviewed
• [Journal Article] Relationships between values of antibodies to several connective tissue disease autoantigens and pulmonary function in a Japanese general population : the Takahata study (2013)
  • Author(s): Nakano H, Shibata Y, Inoue S, Igarashi A, Yamauchi K, Abe S, Sato M, Aida Y, Nunomiya K, Kimura T, Nemoto T, Watanabe T, Konta T, Ueno Y, Kato T, Kayama T, Kubota I
  • Journal Title: PLoS One Volume: 8(12) Pages: e81678
  • Peer Reviewed
• [Journal Article] Relationship between Plasma Fibrinogen Levels and Pulmonary Function in the Japanese Population : The Takahata Study (2013)
  • Author(s): Shibata Y, Abe S, Inoue S, Igarashi A, Yamauchi K, Aida Y, Kishi H, Nunomiya K, Nakano H, Sato M, Sato K, Kimura T, Nemoto T, Watanabe T, Konta T, Ueno Y, Kato T, Kayama T, Kubota I
  • Journal Title: Int J Med Sci. Volume: 10(11) Pages: 1530-1536
  • Peer Reviewed
• [Journal Article] Elevated Serum Iron Is a Potent Biomarker for Spirometric Resistance to Cigarette Smoke among Japanese Males : The Takahata Study (2013)
  • Author(s): Shibata Y, Inoue S, Igarashi A, Yamauchi K, Abe S, Aida Y, Nunomiya K, Sato M, Nakano H, Sato K, Watanabe T, Konta T, Ueno Y, Kato T, Kayama T, Kubota I
  • Journal Title: PLoS One Volume: 8(9) Pages: e74020
  • Peer Reviewed
• [Journal Article] Reduced Number and Morphofunctional Change of Alveolar Macrophages in MafB Gene-Targeted Mice (2013)
  • Author(s): Sato-Nishiwaki M, Aida Y, Abe S, Shibata Y, Kimura T, Yamauchi K, Kishi H, Igarashi A, Inoue S, Sato M, Nakajima O, Kubota I
  • Journal Title: PLoS One Volume: 8(9) Pages: e73963
  • Peer Reviewed
• [Journal Article] Influence of corticosteroid therapy on the serum antibody response to influenza vaccine in elderly patients with chronic pulmonary diseases (2013)
  • Author(s): Inoue S, Shibata Y, Takabatake N, Igarashi A, Abe S, Kubota I
  • Journal Title: EXCLI Journal Volume: 12 Pages: 760-765
  • Peer Reviewed
• [Journal Article] Retrospective Analysis of the Relationship between Decline in FEV(1) and Abdominal Circumference in Male Smokers : the Takahata Study (2013)
  • Author(s): Sato M, Shibata Y, Abe S, Inoue S, Igarashi A, Yamauchi K, Aida Y, Kishi H, Nunomiya K, Nakano H, Sato K, Watanabe T, Konta T, Ueno Y, Kato T, Kayama T, Kubota I
  • Journal Title: Int J Med Sci. Volume: 10(1) Pages: 1-7
  • Peer Reviewed
• [Journal Article] Hyperhomocysteinaemia predicts the decline in pulmonary function in healthy male smokers (2013)
  • Author(s): Nunomiya K, Shibata Y, Abe S, Inoue S, Igarashi A, Yamauchi K, Aida Y, Kishi H, Sato M, Watanabe T, Konta T, Ueno Y, Kato T, Yamashita H, Kayama T, Kubota I
  • Journal Title: Eur Respir J. Volume: 42(1) Pages: 18-27
  • Peer Reviewed
• [Journal Article] Oxidized Phospholipid, 1-Palmitoyl- 2-(9'-Oxo-Nonanoyl)-Glycerophosphoc holine (PON-GPC), Produced in the Lung Due to Cigarette Smoking, Impairs Immune Function in Macrophages (2012)
  • Author(s): Kimura T, Shibata Y, Yamauchi K, Igarashi A, Inoue S, Abe S, Fujita K, Uosaki Y, Kubota I
  • Journal Title: Lung. Volume: 190(2) Pages: 169-182
  • Peer Reviewed
• [Journal Article] Impact of cigarette smoking on maximal expiratory flows in a general population : the Takahata study (2011)
  • Author(s): Nemoto T, Shibata Y, Osaka D, Abe S, Inoue S, Tokairin Y, Igarashi A, Yamauchi K, Kimura T, Kishi H, Nishiwaki M, Aida Y, Nunomiya K, Sato M, Watanabe T, Konta T, Kawata S, Kato T, Kayama T, Kubota I
  • Journal Title: Intern Med. Volume: 50(21) Pages: 2547-2555
  • Peer Reviewed
• [Journal Article] Impairment of Pulmonary Function is an Independent Risk Factor for Atrial Fibrillation : The Takahata Study (2011)
  • Author(s): Shibata Y, Watanabe T, Osaka D, Abe S, Inoue S, Tokairin Y, Igarashi A, Yamauchi K, Kimura T, Kishi H, Aida Y, Nunomiya K, Nemoto T, Sato M, Konta T, Kawata S, Kato T, Kayama T, Kubota I
  • Journal Title: Int J Med Sci. Volume: 8(7) Pages: 514-522
  • Peer Reviewed
• [Journal Article] The Relationship between Serum Uric Acid and Spirometric Values in Participants in a Health Check : The Takahata Study (2011)
  • Author(s): Aida Y, Shibata Y, Osaka D, Abe S, Inoue S, Fukuzaki K, Tokairin Y, Igarashi A, Yamauchi K, Nemoto T, Nunomiya K, Kishi H, Sato M, Watanabe T, Konta T, Kawata S, Kato T, Kubota I
  • Journal Title: Int J Med Sci. Volume: 8(6) Pages: 470-478
  • Peer Reviewed
• [Presentation] The difference in the expression of Ern1 and the formation of granulomas in SPC-CCL1 Tg mice (2014)
  • Author(s): Sato M, Shibata Y, Kishi H, Abe S, Igarashi A, Yamauchi K, Aida Y, Nakano H, Sato K, Kimura T, Nunomiya K, Nemoto T, Inoue S, Kubota I
  • Organizer: American Thoracic Society 2014 International Conference
  • Place of Presentation: San Diego USA, (San Diego Convention Center)
  • Year and Date: 20140516-21
• [Presentation] Lung-Specific CCL1 Overexpression Decreases Alveolar Macrophages In Murine BAL (2012)
  • Author(s): Kishi H, Igarashi A, Abe S, Yamauchi K, Sato M, Nemoto T, Nunomiya K, Aida Y, Inoue S, Kubota I, Shibata Y
  • Organizer: American Thoracic Society 2012 International Conference
  • Place of Presentation: San Francisco USA, (Moscone Convention Center)
  • Year and Date: 20120518-23
• [Presentation] 肺特異的CCL1高発現マウスにおける生体内細胞分画の検討 (2012)
  • Author(s): 岸宏幸,阿部修一,柴田陽光,五十嵐朗,山内啓子,佐藤正道,根本貴子,布宮慶子,會田康子,井上純人,久保田功
  • Organizer: 第52回日本呼吸器学会学術講演会
  • Place of Presentation: 神戸(神戸コンベンションセンター)
  • Year and Date: 20120420-22