2013 Fiscal Year Final Research Report
A role of p116Rip protein in the actin-myosin binding protein transgenic mouse on interstitial pneumonia
| Project/Area Number |
23591135
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Gunma University |
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Principal Investigator |
KOGA YASUHIKO 群馬大学, 医学(系)研究科(研究院), 助教 (10533862)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 非閉塞性肺疾患癌 / 肺線維症 / 呼吸器感染症 |
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| Research Abstract |
We examined the effect of specific siRNA against p116Rip on its protein expression level in human bronchial culture cells, HFL-1 ,A549 and HBEC-3 by western blot. The elimination of p116Rip protein expression was successfully performed using siRNA against p116Rip. Knocking down of p116Rip was accompanied with the elevation of myosin light chain phosphorylation in cells. Furthermore, we generated p116Rip-transgenic mice and examined the effect of p116Rip protein on the Bleomycin-induced acute lung injury model mice. However, p116Rip transgenic mice showed the minimal overexpression of exogenous p116Rip compared with wild type mouse, thus it is thought that we need to examine for further experiments to produce p116Rip transgenic mouse.
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Research Products
(9 results)
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[Journal Article] Phase II study of oral S-1 plus cisplatin with bevacizumab for advanced non-squamous non-small cell lung cancer2013
Author(s)
Kaira K, Tomizawa Y, Yoshino R, Miura Y, Yoshii A, Iwasaki Y, Koga Y, Ono A, Hisada T, Minato K, Sato K, Kazama T, Ishihara S, Kohyama K, Fueki N, Saito R, Sunaga N
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Journal Title
Lung Cancer
Volume: 13
Pages: S0169-5002
Peer Reviewed
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[Journal Article] Phase II study of oral S-1 and cisplatin with concurrent radiotherapy for locally advanced non-small-cell lung cancer2013
Author(s)
Kaira K, Tomizawa Y, Yoshino R, Yoshii A,Matsuura M, Iwasaki Y, Koga Y, Ono A, Nishioka M, Kamide Y, Hisada T, Ishizuka T, Shirai K, Ebara T, Saito J, Nakano T, Sunaga N
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Journal Title
Lung Cancer
Volume: 3 (Epub 2013 Sep 19)
Pages: 449-54
Peer Reviewed
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[Journal Article] Extracellular acidification induces connective tissue growth factor production through proton-sensing receptor OGR1 in human airway smooth muscle cells2011
Author(s)
Matsuzaki S, Ishizuka T, Yamada H, Kamide Y, Hisada T, Ichimonji I, Aoki H, Yatomi M, Komachi M, Tsurumaki H, Ono A, Koga Y, Dobashi K, Mogi C, Sato K, Tomura H, Mori M, Okajima F
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Journal Title
Biochem Biophys Res Commun
Volume: 413(4) (Epub 2011 Sep 1)
Pages: 499-503
Peer Reviewed
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