Analysis of microRNA for the diagnosis and treatment of lung cancer

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591144
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Nagoya University
• Principal Investigator: KONDO Masashi 名古屋大学, 医学(系)研究科(研究院), 准教授 (00378077)
• Co-Investigator(Kenkyū-buntansha): SATO Mitsuo 名古屋大学, 医学部附属病院, 講師 (70467281)
• Project Period (FY): 2011 – 2013
• Keywords: マイクロリボ核酸 / 肺癌

Research Abstract

MicroRNA-127 (miR-127) is located in 14q32 region and the deletion in this region has been reported in lung cancer as well as gastric cancer and hepatocellular carcinoma. The reduced expressions of miR-127 were observed in most of lung cancer cell lines, compared the immortalized normal human bronchial epithelial cell by real time PCR method with Taqman microRNA assay. The overexpression of miR-127 in two lung cancer cell lines, leaded to tumor growth inhibition. Moreover, mRNA microarray analysis of H1975 cancer cell lines after transfection of miR-127 mimic indicated the candidate target genes of miR-127. These results suggested that miR-127 have tumor suppressive effect in non-small-cell lung cancer.

Research Products

• [Journal Article] TIMELESS is overexpressed in lung cancer and its expression correlates with poor patient survival (2013)
  • Author(s): Yoshida K, Sato M, Hase T, Elshazley M, Yamashita R, Usami N, Taniguchi T, Yokoi K, Nakamura S, Kondo M, Girard L, Minna JD, Hasegawa Y
  • Journal Title: Cancer Sci Volume: 104 Pages: 171-7
  • Peer Reviewed
• [Journal Article] Efficacy of Chemotherapy for Advanced Non-Small Cell Lung Cancer with Idiopathic Pulmonary Fibrosis (2013)
  • Author(s): Watanabe N, Taniguchi H, Kondoh Y, Kimura T, Kataoka K, Nishiyama O, Kondo M, Hasegawa Y
  • Journal Title: Respiration Volume: 85 Pages: 326-31
  • Peer Reviewed
• [Journal Article] Genetic polymorphisms of the adenosine triphosphate-binding cassette transporters (ABCG2, ABCB1) and gefitinib toxicity (2012)
  • Author(s): Tamura M, Kondo M, Horio M, Ando M, Saito H, Yamamoto M, Horio Y, Hasegawa Y
  • Journal Title: Nagoya J Med Sci Volume: 74 Pages: 133-40
  • Peer Reviewed
• [Journal Article] The circadian clock gene BMAL1 is a novel therapeutic target for malignant pleural mesothelioma (2012)
  • Author(s): Elshazley M, Sato M, Hase T, Yamashita R, Yoshida K, Toyokuni S, Ishiguro F, Osada H, Sekido Y, Yokoi K, Usami N, Shames DS, Kondo M, Gazdar AF, Minna JD, Hasegawa Y
  • Journal Title: Int J Cancer Volume: 131 Pages: 2820-31
  • Peer Reviewed
• [Journal Article] Transient but Not Stable ZEB1 Knockdown Dramatically Inhibits Growth of Malignant Pleural Mesothelioma Cells (2012)
  • Author(s): Horio M, Sato M, Takeyama Y, Elshazley M, Yamashita R, Hase T, Yoshida K, Usami N, Yokoi K, Sekido Y, Kondo M, Toyokuni S, Gazdar AF, Minna JD, Hasegawa Y
  • Journal Title: Ann Surg Oncol Volume: 19 Pages: 634-45
  • Peer Reviewed
• [Presentation] 非小細胞肺癌におけるmiR-127の機能解析 (2014)
  • Author(s): 丸山英一、各務智弘、梶川茂久、山下良、佐藤光夫、近藤征史、長谷川好規
  • Organizer: 第54回呼吸器学会
  • Place of Presentation: 大阪
  • Year and Date: 2014-04-25
• [Presentation] 時計遺伝子Bmal1ノックダウンはアポトーシスと細胞老化を介して肺癌細胞の増殖を抑性する (2013)
  • Author(s): 山下良、佐藤光夫、Momen Elshazley、長谷哲成、丸山英一、梶川茂久、各務智彦、近藤征史、長谷川好規
  • Organizer: 第54回日本肺癌学会
  • Place of Presentation: 東京千代田区
  • Year and Date: 2013-11-21
• [Presentation] Silencing TIMELESS induceds growth suppression, apoptosis and enhanced cytotoxicity of cisplation in H157 lung cancer cell lines (2012)
  • Author(s): Yoshida K, Sato M, Hase T, Elshazley M, Yamashita R, Usami N, Taniguchi T, Yokoi K, Nakamura S, Kondo M, Girard L, Minna JD, Hasegawa Y
  • Organizer: 2012年アメリカ癌学会
  • Place of Presentation: 米国シカゴ
  • Year and Date: 2012-04-04