2013 Fiscal Year Final Research Report
Examination of evidence of causative pathogen in bacterial pneumonia using clone library analysis
| Project/Area Number |
23591173
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
HIROSHI Mukae 産業医科大学, 医学部, 教授 (80253821)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 細菌叢解析 / 市中肺炎 / 医療介護関連肺炎 / 16S rRNA / 口腔内常在菌 / 嫌気性菌 |
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| Research Abstract |
We evaluated causative pathogens of pneumonia in patients diagnosed with community-acquired pneumonia or healthcare-associated pneumonia using the clone library method as a cultivation-independent method using 16S ribosomal RNA gene sequencing analysis compared to conventional cultivation methods. Our result showed that relatively higher rates of anaerobes and oral bacteria were detected by the molecular method, especially in the elderly, than previously reported. It is speculated that the molecular method we used is a useful tool for detecting causative pathogens of pneumonia, and these bacteria may play more important roles in lower respiratory tract infections.
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