2013 Fiscal Year Final Research Report
Analysis of activation of glomerular parietal epithelial cells to podocyte injury
| Project/Area Number |
23591176
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Kidney internal medicine
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
SASAKI Satoshi 北海道大学, 医学(系)研究科(研究院), 客員研究員 (70312345)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 糸球体硬化症 / 慢性腎疾患 / 糸球体上皮細胞 |
|---|
| Research Abstract |
Recent investigations have disclosed that activation of parietal epithelial cells (PECs) plays key roles in the process of focal segmental glomerulosclerosis (FSGS). In this experimental project, we have tried to investigate dynamic behavior of activated PECs in the evolution of FSGS. We conducted serial assessment of activated PECs using the mouse models of lipopolysaccharide(LPS)-induced proteinuria and adriamycin (ADR) nephrosis. To further analyze the role of activated PECs, we induced rapid progressive podocytopathy using LPS-treated ADR nephrosis mice. As the cell markers for activation, we utilized two markers , CD44 and CXCR4. Using models of ADR nephrosis in mice of different ages, we found that CD44+PECs play significant roles in progressive glomerulosclerosis and the prevalence of the cells reflects different degrees of podocyte injury. In addition, analysis of two different markers for PEC activation clarified different roles of these markers during cell activation process.
|
