2013 Fiscal Year Final Research Report
Proteome analysis of isolated glomeruli of diabetic nephropathy
| Project/Area Number |
23591198
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Osaka City University |
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Principal Investigator |
ISHIMURA Eiji 大阪市立大学, 医学(系)研究科(研究院), 准教授 (20145775)
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| Co-Investigator(Kenkyū-buntansha) |
KAKEHASHI Anna 大阪市立大学, 大学院医学研究科, 講師 (60382222)
NAKATANI Shinya 大阪市立大学, 大学院医学研究科, 幸喜臨床 研究医 (80701325)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 糖尿病性腎症 / ﾈﾌﾛﾈｸﾁﾝ / 糸球体硬化症 / バイオマーカー |
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| Research Abstract |
Proteome analysis is a new technology which can be used to identify disease-specific proteins. We performed proteome analysis of isolate diabetic glomeruli in human autopsy cases to identify new molecules which are related to diabetic nephropathy. By proteosome analysis, nephronectin, which functions as assembly of extracellular matrix, was demonstrated to be up-regulated in diabetic glomeruli. Immunohistochemistry of renal biopsy specimen revealed that nephronectin was highly expressed in mesangial expansion and nodular glomerulosclerosis of diabetic patients, but not in the glomeruli of non-diabetic patients. Furthermore, there was a significant positive correlation between glomerular sclerosis index and percentages of nephronectin-positive glomeruli of diabetic patients. Our results suggest that nephronectin may play an important role in the progression of diabetic nephropathy. Serum and urine concentration of nephronectin is currently performed.
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