Pathophysiology of secretary form of VLDL receptor

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591335
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: University of Fukui
• Principal Investigator: TAKAHASHI Sadao 福井大学, 医学部, 特別研究員 (50303376)
• Project Period (FY): 2011 – 2013
• Keywords: VLDL受容体 / 選択的スプライシング / ヒト血中分泌型VLDL受容体

Research Abstract

In vivo and in vitro studies have shown that the VLDL receptor bind TG-rich lipoprotein (TGRL) but not LDL and function as a peripheral lipoprotein receptor even though the LDL receptor bind both TGRL and LDL. Two VLDL receptor proteins (type1 and type2) are produced by alternative splicing mechanism. We elucidated that type2 VLDL receptor lacking the O-linked sugar domain bound lower affinity for TGRL than type1 VLDL receptor. Type1 VLDL receptor was expressed predominantly during monocyte-macrophage differentiation and growth. Type2 VLDL receptor was secreted into medium and we established the method for measurement of human secertary form of VLDL receptor (sVLDL-R). Level of human sVLDL-R in diabetes was higher than healthy control.

Research Products

• [Journal Article] Differential effects ofα-glucosidase inhibitors on postprandial plasma glucose and lipid profile in patients with type 2 diabetes under control with insulin Lispro mix 50/50 (2012)
  • Author(s): Kimura T, Suzuki J, Ichikawa M, Imagawa M, Sato S, Fujii M, Zenimaru Y, Inaba S, Takahashi S, Konoshita T, Miyamori I
  • Journal Title: Diabetes Technol. Ther. Volume: 14 Pages: 545-551
  • Peer Reviewed
• [Journal Article] Comparative reactivity of remnant-like lipoprotein particles (RLP) and low-density lipoprotein (LDL) to LDL receptor and VLDL receptor : Effect of a high-dose statin on VLDL receptor expression (2012)
  • Author(s): Imagawa M, Takahashi S, Zenimaru Y, Kimura T, Suzuki J, Miyamori I, Iwasaki T, Hattori H, Yamamoto TT, Nakano T, Nakajima K
  • Journal Title: Clin Chim Acta Volume: 413 Pages: 441-447
  • Peer Reviewed
• [Journal Article] Species differences of macrophage very low-density-lipoprotein (VLDL) receptor protein expression (2011)
  • Author(s): Takahashi S, Ito T, Zenimaru Y, Suzuki J, Miyamori I, Takahashi M, Takahashi M, Ishida T, Ishida T, Hirata K, Yamamoto TT, Iwasaki T, Hattori H, Shiomi M
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 407 Pages: 656-662
  • Peer Reviewed
• [Presentation] 分泌型VLDL受容体の存在とヒト血中濃度測定法の確立 (2013)
  • Author(s): 高橋貞夫,銭丸康夫鈴木仁弥今川美智子佐藤さつき山本勝司,山田実夏市川麻衣石塚全,服部浩明
  • Organizer: 第17回日本心血管内分泌代謝学会学術総会
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
  • Year and Date: 20131122-23
• [Presentation] Presence of human soluble very-low-density lipoprotein (VLDL) receptor in serum (2012)
  • Author(s): Takahashi S, Zenimaru Y, Imagawa M, Kimura T, Ichikaewa M, Sato S, Suzuki J, Miyamori I, Iwasaki T, Hattori H
  • Organizer: XVI International Symposium on Atherosclerosis
  • Place of Presentation: Sydney Convention and Exhibition Centre, Australia
  • Year and Date: 20120325-29
• [Presentation] Central role of lipoprotein lipase and very low-density lipoprotein receptor on macrophage foam cell formation by triglyceride-rich lipoproteins and low density lipoprotein (2011)
  • Author(s): Takahashi S, Zenimaru Y, Imagawa M, Kimura T, Suzuki J, Miyamori I
  • Organizer: 47^<th> EASD Annual Meeting
  • Place of Presentation: Lisbon
  • Year and Date: 20110912-16
• [Presentation] Species differences of macrophage very low-density lipoprotein (VLDL) receptor protein expression (2011)
  • Author(s): Takahashi S, Zenimaru Y, Imagawa M, Kimura T, Suzuki J, Miyamori I, Yamamoto TT, Ito T, Shiomi M
  • Organizer: AHA, ATVB meeting 2011
  • Place of Presentation: Chicago Hilton Hotel, Chicago
  • Year and Date: 20110418-20
• [Book] VLDLR:疾患モデルの作製と利用—脂質代謝異常と関連疾患 (2014)
  • Author(s): 高橋貞夫
  • Publisher: エル・アイ・シー出版(印刷中)