2013 Fiscal Year Final Research Report
The effects of omega-3 PUFA on vascular function
| Project/Area Number |
23591336
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Kagoshima University |
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Principal Investigator |
NISHIO Yoshihiko 鹿児島大学, 医歯(薬)学総合研究科, 教授 (40281084)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 酸化ストレス / 血管内皮 |
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| Research Abstract |
We tested whether n-3 PUFAs showed antioxidant activity through the activation of Nrf2, a master transcriptional factor for antioxidant genes. C57BL/6 or Nrf2-/- mice were fed a fish-oil diet. Fish-oil diet significantly increased the expression of heme oxygenase-1 (HO-1), and endothelium-dependent vasodilation in the aorta of C57BL/6 mice, but not in Nrf2-/- mice. Furthermore, we observed that 4-hydroxy hexenal (4-HHE), an end-product of n-3 PUFA peroxidation, was significantly increased in the aorta of C57BL/6 mice, accompanied by intra-aortic predominant increase in DHA rather than that in EPA. Human umbilical vein endothelial cells were incubated with DHA or EPA. We found that DHA, but not EPA, markedly increased intracellular 4-HHE, and nuclear expression and DNA binding of Nrf2. Both DHA and 4-HHE also increased the expressions of Nrf2 target genes including HO-1. Furthermore, DHA prevented oxidant-induced cellular damage and this effect was disappeared by the siRNA of Nrf2.
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Research Products
(8 results)
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[Journal Article] A fish-based diet intervention improves endothelial function in postmenopausal women with type 2 diabetes mellitus : A randomized crossover trial2014
Author(s)
Kondo K, Morino K, Nishio Y, Kondo M, Nakao K, Nakagawa F, Ishikado A, Sekine O, Yoshizaki T, Kashiwagi A, Ugi S, Maegawa H
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Journal Title
Metabolism
Volume: (In press)
Peer Reviewed
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[Journal Article] 4-Hydroxy hexenal derived from dietary n-3 polyunsaturated fatty acids induces anti-oxidative enzyme heme oxygenase-1 in multiple organs2014
Author(s)
Nakagawa F, Morino K, Ugi S, Ishikado A, Kondo K, Sato D, Konno S, Nemoto K, Kusunoki C, Sekine O, Sunagawa A, Kawamura M, Inoue N, Nishio Y, Maegawa H
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Journal Title
Biochem Biophys Res Commun
Volume: 443(3)
Pages: 991-6
Peer Reviewed
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[Journal Article] 4-Hydroxy hexenal derived from docosahexaenoic acid protects endothelial cells via Nrf2 activation2013
Author(s)
Ishikado A, Morino K, Nishio Y, Nakagawa F, Mukose A, Sono Y, Yoshioka N, Kondo K, Sekine O, Yoshizaki T, Ugi S, Uzu T, Kawai H, Makino T, Okamura T, Yamamoto M, Kashiwagi A, Maegawa H
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Journal Title
PLoS One
Volume: 8(7)
Pages: e69415
Peer Reviewed
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[Journal Article] Ezetimibe prevents hepatic steatosis induced by a high-fat but not a high-fructose diet2013
Author(s)
Ushio M, Nishio Y, Sekine O, Nagai Y, Maeno Y, Ugi S, Yoshizaki T, Morino K, Kume S, Kashiwagi A, Maegawa H
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Journal Title
Am J Physiol Endocrinol Metab
Volume: 305(2)
Pages: E293-304
Peer Reviewed
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[Journal Article] Omega-3 polyunsaturated fatty acid has an anti-oxidant effect via the Nrf-2/HO-1 pathway in 3T3-L1 adipocytes2013
Author(s)
Kusunoki C, Yang L, Yoshizaki T, Nakagawa F, Ishikado A, Kondo M, Morino K, Sekine O, Ugi S, Nishio Y, Kashiwagi A, Maegawa H
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Journal Title
Biochem Biophys Res Commun
Volume: 430(1)
Pages: 225-30
Peer Reviewed
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