2013 Fiscal Year Final Research Report
Flexibility of intracellular estrogen signaling pathway and mechanism of aromatase inhibitor-resistance
| Project/Area Number |
23591344
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Tohoku University |
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Principal Investigator |
HAYASHI Shinichi 東北大学, 医学(系)研究科(研究院), 教授 (60144862)
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| Project Period (FY) |
2011 – 2013
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| Keywords | エストロゲン / ホルモン療法耐性 / アロマターゼ阻害薬 / MCF-7細胞 / 乳癌 / シグナル伝達 / GFP / アンドロゲン |
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| Research Abstract |
To elucidate the aromatase inhibitor (AI)-resistant mechanism, we established 6 kinds of resistant cell lines having different resistant mechanisms from ERE-GFP introduced MCF-7 cells which we established previously. These resistant cells were established by long-term estrogen depletion and/or androgen supplemented under monitoring ERE-GFP activity. The characterization of these cell lines revealed novel hormonal therapy-resistant mechanisms such as ligand-independent ER activation by phosphorylation from PI3K-Akt pathway, ER activation by estrogen-like androgen metabolite (3bHSD), or androgen receptor-dependent growth mechanism.
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[Journal Article] Estrogen response element-GFP (ERE-GFP) introduced MCF-7 cells demonstrated the coexistence of multiple estrogen- deprivation resistant mechanisms2014
Author(s)
Fujiki N, Konno H, Kaneko Y, Gohno T, Hanamura T, Imami K, Ishihama Y, Nakanishi K, Niwa T, Seino Y, Yamaguchi Y, Hayashi S
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Journal Title
J. Steriod Biochem. Mol. Biol.
Volume: 139
Pages: 61-72
DOI
Peer Reviewed
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[Journal Article] Androgen metabolite- dependent growth of hormone receptor- positive breast cancer as a possible aromatase inhibitor-resistance mechanism2013
Author(s)
Hanamura T, Niwa T, Nishikawa S, Konno H, Gohno T, Tazawa C, Kobayashi Y, Kurosumi M, Takei H, Yamaguchi Y, Ito K, Hayashi S
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Journal Title
Breast Cancer Res. Treat
Volume: 139(3)
Pages: 731-740
DOI
Peer Reviewed
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[Journal Article] Intratumoral estrogen concentration and expression of estrogen-induced genes in male breast carcinoma : comparison with female breast carcinoma2013
Author(s)
Takagi K, Moriya T, Kurosumi M, Oka K, Miki Y, Ebata A, Toshima T, Tsunekawa S, Takei H, Hirakawa H, Ishida T, Hayashi S, Kurebayashi J, Sasano H, Suzuki T
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Journal Title
Hormones and Cancer
Volume: 4(1)
Pages: 1-11
DOI
Peer Reviewed
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[Journal Article] Individual transcription activity of estrogen receptors in primary breast cancer and its clinical significance2012
Author(s)
Gohno T, Seino Y, Hanamura T, Niwa T, Matsumoto M, Yaegashi N, Oba H, Kurosumi M, Takei H, Yamaguchi Y, Hayashi S
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Journal Title
Cancer Medicine
Volume: 1(3)
Pages: 328-337
DOI
Peer Reviewed
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[Journal Article] Identification of a GPER/GPR30 antagonistwithimproved estrogen receptor counterselectivity2011
Author(s)
Dennis MK, Field AS, Burai R, Ramesh C, Petrie WK, Bologa CG, Oprea TI, Yamaguchi Y, Hayashi S, Sklar SLA, Hathaway HJ, Arterburn, JB, and Prossnitz ER
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Journal Title
J. Steroid Biochem. Mol. Biol.
Volume: 127
Pages: 358-366
DOI
Peer Reviewed
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[Presentation] The androgen metabolite-dependent growth of hormone receptor positive breast cancer as a possible aromatase inhibitor-resistance mechanism2013
Author(s)
Toru Hanamura, Toshifumi Niwa, Sayo Nishikawa, Hiromi Konno, Tatsuyuki Gohno, Chika Tazawa, Yasuhito Kobayashi, Masafumi Kurosumi, Hiroyuki Takei, Yuri Yamaguchi, Ken-ichi Ito, Shin-ichi Hayashi
Organizer
American Association for Cancer Research ANNUAL MEETING 2013
Place of Presentation
Washington
Year and Date
20130406-10
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[Presentation] The androgen metabolite-dependent growth in hormone receptor positive breast cancer as a novel aromatase inhibitor-resistance mechanism2012
Author(s)
Toru Hanamura, Toshifumi Niwa, Sayo Nishikawa, Hiromi Konno, Tatsuyuki Ghono, Yasuhito Kobayashi, Masafumi Kurosumi, Hiroyuki Takei, Yuri Yamaguchi, Ken-ichi Ito, Shin-ichi Hayashi
Organizer
Annual San Antonio Breast Cancer Symposium
Place of Presentation
San Antonio,USA
Year and Date
20121204-08
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