2013 Fiscal Year Final Research Report
Visualization of exocytosis from neuroendocrine cells and neuroendoceine tumor cells.
| Project/Area Number |
23591348
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Kitasato University (2013)
The University of Tokyo (2011-2012) |
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Principal Investigator |
TAKANO Koji 北里大学, 医学部, 准教授 (20236243)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ホルモン分泌 / 開口分泌 / 神経内分泌 / 下垂体 / 可視化解析 |
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| Research Abstract |
The exocytosis by anterior pituitary cells were visualized by TEP technique using two-photon excitation. The spontaneous exocytosis by rat lactotroph was rapid full fusion that terminated within a second while GHRH-stimulated exocytosis by rat somatotroph was slower full fusion that terminated within 3-6 seconds. D2 agonist and blockers of voltage-gated calcium channels inhibited spontaneous exocytosis, indicating that the observed spontaneous exocytosis corresponds to the spontaneous prolactin secretion from anterior pituitary when liberated from hypothalamic inhibition by dopamine. Somatostatin, that is a physiological hypothalamic inhibitor of GH secretion, attenuated GHRH-induced exocytosis. Somatostatin also inhibited high K-induced GH exocytosis. Because somatostatin did not abolish high K-induced intracellular calcium increase, somatostatin may inhibit GH exocytosis by acting on distal mechanism to calcium influx.
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[Journal Article] DNA mismatch repair protein MSH6 correlated with the responses of atypical pituitary adenomas and pituitary carcinomas to temozolomide : The national cooperative study by the Japan Society for Hypothalamic and Pituitary Tumors2013
Author(s)
Hirohata, T., Asano, K., Takano, K., Takano, S., Arita, K., Tahara, S., et al.
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Journal Title
J. Clin. Endocrinol. & Metab.
Volume: 98(3)
Pages: 1130-1136
DOI
Peer Reviewed
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[Journal Article] Urinary growth hormone level and insulin-like growth factor-1 standard deviation score (IGF-SDS) can discriminate adult patients with severe growth hormone deficiency2012
Author(s)
Hirohata T, Saito N, Takano K, Yamada S, Son JH, Yamada SM, Nakaguchi H, Hoya K, Murakami M, Mizutani A, Okinaga H, Matsuno A
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Journal Title
Endocr J
Volume: 60
Pages: 369-373
Peer Reviewed
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[Journal Article] Development and Validation of a New Questionnaire Assessing Quality of Life in Adults with Hypopituitarism : Adult Hypopituitarism Questionnaire (AHQ)2012
Author(s)
Ishii, H., Shimatsu, A. , Okimura, Y., Tanaka, T., Hizuka, N., Kaji, H., Hanew, K., Oki, Y., Yamashiro, Y, Takano, K., Chihara, K.
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Journal Title
PLoS ONE
Volume: 7(9)
Pages: e44304
DOI
Peer Reviewed
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[Journal Article] 小児期から成人期へGH分泌不全症の移行期医療を考える2012
Author(s)
島津章,香川二郎,都研一,高橋裕,堀川玲子,高野幸路,中村明枝,沼倉周彦,北中幸子,長崎啓祐,河野斉,西美和
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Journal Title
内分泌・糖尿病・代謝内科
Volume: 35巻3号
Pages: 275-283
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[Journal Article] Molecular Morphology of Pituitary Cells, from Conventional immunohistochemistry to Fluorescein Imaging2011
Author(s)
Matsuno, A., Mizutani, A., Okinaga, H., Takano, K., Yamada, S., Yamada, S.M., Nakaguchi, H., Hoya, K., Murakami, M., Takeuchi, M., Sugaya, M., Itoh, J., Takekoshi, S., Osamura, R.Y.
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Journal Title
Molecules
Volume: 16
Pages: 3618-3635
DOI
Peer Reviewed
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[Journal Article] Functional molecular morphology of anterior pituitary cells, from hormone production to intracellular transport and secretion2011
Author(s)
Matsuno, A., Mizutani, A., Okinaga, H., Takano, K., Yamada, S., Yamada, S.M., Nakaguchi, H., Hoya, K., Murakami, M., Takeuchi, M., Sugaya, M., Itoh, J., Takekoshi, S., Osamura, R.Y.
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Journal Title
Med Mol Morphol
Volume: 44
Pages: 63-70
DOI
Peer Reviewed
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[Journal Article] A mechanism of acquiring temozolomide resistance during transformation of atypical prolactinoma into prolactin-producing pituitary carcinoma2011
Author(s)
Murakami, M., Mizutani, A., Asano, S., Katakami, H., Ozawa, Y., Yamazaki, K., Ishida, Y., Takano, K., Okinaga, H., Matsuno, A.
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Journal Title
Neurosurgery
Volume: 68
Pages: E1761-1767
DOI
Peer Reviewed
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