Detection for miRNA dysregulation in CML

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591365
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Akita University
• Principal Investigator: TAKAHASHI Naoto 秋田大学, 医学(系)研究科(研究院), 講師 (80344753)
• Co-Investigator(Kenkyū-buntansha): TAGAWA Hiroyuki 秋田大学, 大学院医学系研究科, 講師 (30373492)
• Project Period (FY): 2011 – 2013
• Keywords: CML / miRNA / miR-200c / Bmi-1

Research Abstract

To detect specific miRNA in cancer stem cell of chromic myelogenious leukemia (CML), we established CML mice (B6) which can develop CML. We got cancer stem cells from B6 mice and examined miRNA expression from KLS population. We found that several miRNA were up-or down regulated in these mice. For instance, down regulation of miR-203,miR-200c, miR-26,miR-21,miR-2105, and miR-181. In this time we are examining targets of these miRNAs. We identified Bmi-1 is possible target of miR-200c in CML stem cells. And we fort her demonstrated that bortezomib was effective for diminishing CML stem cells by targeting both Bmi-1 and miR-200c.

Research Products

• [Journal Article] Bortezomib reduces the tumorigenicity of multiple myeloma via downregulation of upregulated targets in clonogenic side population cells (2013)
  • Author(s): Nara M, Teshima K, Watanabe A, Ito M, Iwamoto K, Kitabayashi A, Kume M, Hatano Y, Takahashi N, Iida S, Sawada K, Tagawa H
  • Journal Title: PLoS One Volume: 8(3) Pages: e56954
• [Journal Article] Enucleation of human erythroblasts involves non-muscle myosin IIB (2012)
  • Author(s): Ubukawa K, Guo YM, Takahashi M, Hirokawa M, Michishita Y, Nara M, Tagawa H, Takahashi N, Komatsuda A, Nunomura W, Takakuwa Y, Sawada K
  • Journal Title: Blood Volume: 119 Pages: 1036-44
• [Journal Article] A synthetic double-stranded RNA, poly I : C, induces a rapid apoptosis of human CD34(+) cells (2012)
  • Author(s): Liu J, Guo YM, Hirokawa M, Iwamoto K, Ubukawa K, Michishita Y, Fujishima N, Tagawa H, Takahashi N, Xiao W, Yamashita J, Ohteki T, Sawada K
  • Journal Title: Exp Hematol Volume: 40 Pages: 330-41
• [Journal Article] False-positive human immunodeficiency virus antibody test and autoimmune hemolytic anemia in a patient with angioimmunoblastic T-cell lymphoma (2011)
  • Author(s): Shida S, Takahashi N, Fujishima N, Kameoka Y, Nara M, Fujishima M, Saitoh H, Tagawa H, Hirokawa M, Ichinohasama R, Sawada K
  • Journal Title: Intern Med Volume: 50(20) Pages: 2383-7
• [Journal Article] Age-associated alteration ofγδT-cell repertoire and different profiles of activation-induced death of Vδ1 and Vδ2 T cells (2011)
  • Author(s): Michishita Y, Hirokawa M, Guo YM, Abe Y, Liu J, Ubukawa K, Fujishima N, Fujishima M, Yoshioka T, Kameoka Y, Saito H, Tagawa H, Takahashi N, Sawada K
  • Journal Title: Int J Hematol Volume: 94 Pages: 230-40
• [Journal Article] The role of microRNA-150 as a tumor suppressor in malignant lymphoma (2011)
  • Author(s): Watanabe A, Tagawa H, Yamashita J, Teshima K, NaraM, Iwamoto K, Kume M, Kameoka Y, Takahashi N, Nakagawa T, Shimizu N, Sawada K
  • Journal Title: Leukemia Volume: 25 Pages: 1324-34
• [Journal Article] Fatal hemorrhagic pneumonia caused by Stenotrophomanas maltophilia in a patient with non-Hodgkin lymphoma (2011)
  • Author(s): Takahashi N, Yoshioka T, Kameoka Y, Tagawa H, Fujishima N, Saitoh H, Hirokawa M, Enomoto K, Sawada K
  • Journal Title: J Infect Chemother Volume: 17 Pages: 858-62
• [Journal Article] Drug interaction of (S)-warfarin, and not (R)-warfarin, with itraconazole in a hematopoietic stem cell transplant recipient (2011)
  • Author(s): Miura M, Takahashi N, Kanno S, Kato S, Nara M, Itoh M, Saitoh H, Yoshioka T, Kameoka Y, Fujishima N, Tagawa H, Hirokawa M, Sawada K
  • Journal Title: Clin Chim Acta Volume: 9;412 Pages: 2002-6
• [Journal Article] Successful treatment of necrotizing fasciitis in an upper extremity caused by Clostridium perfringens after bone marrow transplantation (2011)
  • Author(s): Ito M, Takahashi N, Saitoh H, Shida S, Nagao T, Kume M, Kameoka Y, Tagawa H, Fujishima N, Hirokawa M, Tazawa H, Minato T, Yamada S, Sawada K.
  • Journal Title: Intern Med Volume: 50 Pages: 2213-7