2013 Fiscal Year Final Research Report
Expression of IL17F induced by a novel cytokine IL-33
| Project/Area Number |
23591455
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | University of Tsukuba |
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Principal Investigator |
KAWAGUCHI MIO 筑波大学, 医学医療系, 講師 (50365748)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 気管支喘息 / IL-17F / IL-33 / 気道上皮細胞 |
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| Research Abstract |
IL-17F is also involved in the pathogenesis of asthma. However, the regulatory mechanisms of IL-17F expression remain to be defined. Therefore we investigated the expression of IL-17F by IL-33 in bronchial epithelial cells, and its signaling mechanisms. IL-33 significantly induced IL-17F gene and protein expression. The receptor for IL-33, ST2, was expressed in bronchial epithelial cells. Among MAP kinases, IL-33 phosphorylated ERK1/2. It was inhibited by the pretreatment of anti-ST2 neutralizing (blocking) Abs. MEK inhibitor significantly blocked IL-17F production. Moreover, IL-33 phosphorylated MSK1, and MEK inhibitor diminished its phosphorylation. Finally, MSK1 inhibitors and transfection of the siRNAs targeting MSK1 significantly blocked IL-17F expression. IL-33 induces IL-17F via ST2-ERK1/2-MSK1 signaling pathway in bronchial epithelial cells. These data suggest that the IL-33/IL-17F axis is involved in allergic airway inflammation, and may be a novel therapeutic target.
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Research Products
(11 results)
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[Journal Article] Potential involvement of IL-17F in asthma2014
Author(s)
Ota K, Kawaguchi M, Matsukura S, Kurokawa M, Kokubu F, Fujita J, Morishima Y, Huang SK, Ishii Y, HSatoh H, Nobuyuki Hizawa N
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Journal Title
Journal of Immunology Research
Volume: Vol.2014
Pages: 1-8
DOI
Peer Reviewed
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[Journal Article] Interleukin-33 induces interleukin- 17F in bronchial epithelial cells2012
Author(s)
Fujita J, Kawaguchi M, Kokubu F, Ohara G, Ota K, Huang SK, Morishima Y, Ishii Y, Satoh H, Sakamoto T, Hizawa N
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Journal Title
Allergy
Volume: Vol.67
Pages: 744-750
DOI
Peer Reviewed
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[Presentation] IL-33 Induces IL-17F In Bronchial Epithelial Cells2012
Author(s)
Fujita J, Kawaguchi M, Kokubu F, Matsukura S, Kurokawa M, Nozato K, Ano S,Kaneko Y, Masuko H, Yamadori T, Yageta Y, Morishima Y, Ishii Y, Sakamoto T, Satoh H, Hizawa N
Organizer
International Conference of American Thoracic Society
Place of Presentation
モスコー二センター、サンフランシスコ、米国
Year and Date
2012-05-20
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[Presentation] 気道上皮細胞からのIL-17F産生2012
Author(s)
藤田純一,川口未央,國分二三男,松倉聡,黒川真嗣,野里恭子,阿野哲士,金子美子,増子裕典,山鳥忠宏,森島祐子,石井幸雄,坂本透,足立満,檜澤伸之
Organizer
第52回日本呼吸器学会学術講演会
Place of Presentation
神戸コンベションセンター、神戸
Year and Date
2012-04-22
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