2013 Fiscal Year Final Research Report

Regulation of intestinal epithelial cell activation by C. difficile lagellin and intestinal commensal bacteria

Research Project

Project/Area Number	23591484
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Infectious disease medicine
Research Institution	Teikyo University
Principal Investigator	OTA Yasuo 帝京大学, 医学部, 教授 (80292936)
Project Period (FY)	2011 – 2013
Keywords	C.difficile / フラジェリン / MDP / CDI / トレランス
Research Abstract	The aim of this study was to elucidate the molecular mechanism of the pathogenesis of CDI, especially the role of C. difficile flagellin. We also examined interaction between C. difficile and the intestinal commensal bacteria on the pathogenesis of CDI. We first analyzed the effect of C. difficile toxin B on IL-8 and CCL20 production. Interestingly, stimulation with toxin B and flagellin induced significantly higher levels of IL-8 and CCL20 compared with individual stimulation. Next, we paid our attention to MDP as stimulant from commensal bacteria. Pretreatment with MDP especially 16 hours before flagellin stimulation lead to significant decrease of IL-8 production, indicating that the cells responded little to flagellin stimulation. These results imply that interaction between C. difficile and the intestinal commensal bacteria might regulate activation of intestinal cells by C. difficile flagellin stimulation.