2013 Fiscal Year Final Research Report
Transcriptome analysis of steroidogenic cells in knockout mice lacking steroidogenic acute regulatory protein
| Project/Area Number |
23591516
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | StAR / 先天性リポイド副腎過形成症 / ノックアウトマウス / 副腎皮質 / ステロイドホルモン / トランスクリプトーム / マクロファージ |
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| Research Abstract |
Steroidogenic acute regulatory protein (StAR) is responsible for congenital lipoid adrenal hyperplasia (StAR deficiency). To delineate a specific pattern of gene expression associated with StAR deficiency, we analyzed the transcriptome of adrenocortical cells isolated by fluorescence-activated cell sorting at E17.5 or E18.5 from seven wild-type and four StAR knockout (Star-/-) mice with transgene targeting eGFP to StAR-expressing cell lineages. We identified that 1,206 and 767 genes were significantly up-regulated and down-regulated, respectively, and that genes involved in cholesterol efflux and inflammatory or immune response were significantly up-regulated in Star-/- mice. An increased number of macrophages were detected in the adrenal glands of Star-/- mice by immunohistochemistry. These results revealed the cholesterol efflux and the inflammatory or immune response as new pathophysiological hallmarks in the adrenocortical cells of Star-/- mice.
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Research Products
(6 results)
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[Presentation] 先天性リポイド副腎過形成症の卵巣にマクロファージが浸潤する2013
Author(s)
石井智弘,福澤龍二,佐藤武志,室谷浩二,安達昌功,井原健二,井垣純子,長谷川行洋,佐藤清二,三井俊賢,長谷川奉延
Organizer
第47回日本小児内分泌学会
Place of Presentation
東京
Year and Date
2013-10-12
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