Transcriptome analysis of microglia using animal model of brain diseases

2014 Fiscal Year Final Research Report

• Project/Area Number: 23591522
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Tokyo Metropolitan Institute of Medical Science (2012-2014); National Center of Neurology and Psychiatry (2011)
• Principal Investigator: SAKUMA Hiroshi 公益財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, 主席研究員 (50425683)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: ミクログリア / 神経炎症 / トランスクリプトーム

Outline of Final Research Achievements

Transcriptome analyses of microglia revealed that not only inflammatory but interferon- and lipid metabolism-associated genes were upregulated at the peak stage of experimental autoimmune encephalomyelitis. Using co-culture model of bone marrow immature cells and astrocytes, interleukin34 proved to be crucial for the proliferation and differentiation of microglia. Microglal adenosine A2A receptor was upregulated in the context of inflammation and negatively regulated interleukin 1β production.

Free Research Field

神経免疫学