Role of Rab family small G proteins in the development of neuroblastoma cancer stem cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591540
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kobe University
• Principal Investigator: NISHIMURA Noriyuki 神戸大学, 医学(系)研究科(研究院), 准教授 (00322719)
• Co-Investigator(Kenkyū-buntansha): HAYAKAWA Akira 神戸大学, 大学院医学研究科, 准教授 (40379376)
• Co-Investigator(Renkei-kenkyūsha): NISHIO Hisahide 神戸大学, 大学院医学研究科, 教授 (80189258)
• Project Period (FY): 2011 – 2013
• Keywords: 神経芽腫 / がん幹細胞 / MRD / Rab

Research Abstract

More than 50% of high-risk neuroblastoma patients have experienced tumor relapses caused by chemoresistant cancer stem cells (CSCs). Consequently, their overall survival rates remain less than 40%. To improve their prognosis, it is essential to understand the molecular mechanism of how CSCs are generated and maintained in neuroblastoma. In the present study, we have focused on the key regulators of membrane traffic, Rab family small G proteins (Rabs), and identified a member of Rabs controlling the development of neuroblastoma CSCs.

Research Products

• [Journal Article] Minimal residual disease monitoring in neuroblastoma patients based on the expression of a set of real-time RT-PCR markers in tumor-initiating cells (2013)
  • Author(s): Hartomo TB, Kozaki A, Hasegawa D, Pham TVH, Yamamoto N, Saitoh A, Ishida T, Kawasaki K, Kosaka Y, Ohashi H, Yamamoto T, Morikawa S, Hirase S, Kubokawa I, Mori T, Yanai T, Hayakawa A, Takeshima Y, Iijima K, Matsuo M, Nishio H, and Nishimura N.
  • Journal Title: Oncol. Rep Volume: 29 Pages: 1629-1636
  • DOI: 10.3892/or.2013.2286
  • Peer Reviewed
• [Journal Article] Rab15 alternative splicing is altered in spheres of neuroblastoma cells (2012)
  • Author(s): Pham TVH, Hartomo TB, Lee MJ, Hasegawa D, Ishida T, Kawasaki K, Kosaka Y, Yamamoto T, Morikawa S, Yamamoto N, Kubokawa I, Mori T, Yanai T, Hayakawa A, Takeshima Y, Iijima K, Matsuo M, Nishio H, and Nishimura N.
  • Journal Title: Oncol. Rep Volume: 27 Pages: 2045-2049
  • DOI: 10.3892/or.2012.1731
  • Peer Reviewed
• [Journal Article] Epigallocatechin gallate inhibits sphere formation of neuroblastoma BE(2)-C cells (2012)
  • Author(s): Nishimura N, Hartomo TB, Pham TVH, Lee MJ, Yamamoto T, Morikawa S, Hasegawa D, Takeda H, Kawasaki K, Kosaka Y, Yamamoto N, Kubokawa I, Mori T, Yanai T, Hayakawa A, Takeshima Y, Iijima K, Matsuo M, and Nishio H.
  • Journal Title: Environ. Health Prev. Med Volume: 17 Pages: 246-251
  • DOI: 10.1007/s12199-011-0239-5
  • Peer Reviewed
• [Journal Article] Rab15 expression correlates with retinoic acid-induced differentiation of neuroblastoma cells (2011)
  • Author(s): Nishimura N, Pham TVH, Hartomo TB, Lee MJ, Hasegawa D, Takeda H, Kawasaki K, Kosaka Y, Yamamoto T, Morikawa S, Yamamoto N, Kubokawa I, Mori T, Yanai T, Hayakawa A, Takeshima Y, Nishio H, and Matsuo M.
  • Journal Title: Oncol. Rep Volume: 26 Pages: 145-151
  • DOI: 10.3892/or.2011.1255
  • Peer Reviewed
• [Presentation] Induction of ALDH1A2 expression is critical for cancer stem cell properties in neuroblastoma (2014)
  • Author(s): Nishimura N, Hartomo TB, Pham TVH, Yamamoto N, Hirase S, Hayakawa A, Hasegawa D, Kawasaki K, Kosaka Y, Matsuo M, Takeshima Y, Iijima K, and Nishio H.
  • Organizer: AACR Annual Meeting 2014
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 20140405-09
• [Presentation] Identification of ALDH1A2 as a critical ALDH isoform in neuroblastoma cells with cancer stem cell phenotype (2013)
  • Author(s): Hartomo TB, Pham TVH, Yamamoto N, Hirase S, Hayakawa A, Hasegawa D, Kawasaki K, Kosaka Y, Matsuo M, Takeshima Y, Iijima K, Nishio H, and Nishimura N.
  • Organizer: AACR Special Conference : Pediatric Cancer at the Crossroads
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 20131103-06
• [Presentation] 神経芽腫症例におけるmultiple real-time RT-PCR markerを用いた微小残存病変(MRD)解析 (2012)
  • Author(s): 田中愛子,長谷川大一郎, Tri Budi Hartomo,山本暢之,宮田憲二,越智聡史,斎藤敦郎,山下達也,石田敏章,川崎圭一郎,松尾雅文, Thi Van Huyen Pham,大橋浩基,森健,矢内友子,早川晶,竹島泰弘,小阪嘉之,飯島一誠,西尾久英,西村範行
  • Organizer: 第54回日本小児血液・がん学会学術集会
  • Place of Presentation: 横浜(口演)
  • Year and Date: 20121130-1202
• [Presentation] 茶カテキンの神経芽腫がん幹細胞に対する抗がん作用の検討 (2012)
  • Author(s): 西村範行, Tri Budi Hartomo,李明鎭,長谷川大一郎,小阪嘉之,早川晶,竹島泰弘,飯島一誠,松尾雅文,西尾久英
  • Organizer: 第82回日本衛生学会学術総会
  • Place of Presentation: 京都(口演)
  • Year and Date: 20120324-26