Pan-genome analysis of virullent and antibiotics-resistant genes among Escherichia coli isolated from diarrheal children

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591574
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kagoshima University
• Principal Investigator: NISHI Junichiro 鹿児島大学, 医歯(薬)学総合研究科, 教授 (40295241)
• Co-Investigator(Kenkyū-buntansha): NOMURA Yuichi 鹿児島大学, 大学院医歯学総合研究科, 准教授 (90237884)
• Co-Investigator(Renkei-kenkyūsha): YAMASAKI Shinji 大阪府立大学, 大学院生命環境科学研究科, 教授 (70221653)
• Project Period (FY): 2011 – 2013
• Keywords: 大腸菌 / 腸管凝集性大腸菌 / ＥＳＢＬ（基質拡張型βラクタマーゼ） / 下痢症 / 小児 / 遺伝子水平伝播 / 下痢原性大腸菌 / 尿路病原性大腸菌

Research Abstract

A total of 6,216 E. coli strains from diarrheal children from 2001 through 2010 in our district were examined for serotyping, ESBL genotyping, and virulence genes including aggR by PCR. In addition, MLSA of ESBL-producing EAEC strains were performed. The incidence of ESBL genes in aggR-positive EAEC strains (20.8%, 31/149) was significantly higher than in other E. coli strains (1.1%, 64/6067) (OR 24.6, 95% Cl 15.5-39.3). ESBL genes were not detected in other pathotypes of diarrheagenic E. coli. All 31 ESBL-producing EAEC strains possessed CTX-M-14, most of which were found to be O25:H4:ST131. EAEC O111 and O126 strains mainly isolated since 1997 through 2010 never harbored ESBL genes, while the EAEC O25:H4:ST131 clone producing CTX-M-14 was detected in 2003 for the first time. These findings suggest that the O25:H4:ST131 clone horizontally acquired CTX-M-14 and EAEC virulence genes in our district. EAEC may play a role of reservoir of ESBL genes.

Research Products

• [Journal Article] Hyporesponsiveness to the infecting serotype after vaccination of children with seven-valent pneumococcal conjugate vaccine following invasive pneumococcal disease (2014)
  • Author(s): Tamura K, Matsubara K, Ishiwada N, Nishi J, Ohnishi H, Suga S, Ihara T, Chang B, Akeda Y, Oishi K, Japanese I. P. D. Study Group
  • Journal Title: Vaccine Volume: 32(13) Pages: 1444-1450
  • Peer Reviewed
• [Journal Article] Antimicrobial effect of an ultrasonic levitation washer disinfector with silver electrolysis and ozone oxidation on methicillin-resistant Staphylococcus aureus (MRSA) (2013)
  • Author(s): Tamai M, Matsushita S, Miyanohara H, Imuta N, Ikeda R, Kawai K, Nishi J, Sakamoto A, Shigihara T, Kanekura T
  • Journal Title: J Dermatol Volume: 40(12) Pages: 1020-1026
  • Peer Reviewed
• [Journal Article] Ramsay hunt syndrome in a girl with acute lymphoblastic leukemia during maintenance therapy (2013)
  • Author(s): Kodama Y, Okamoto Y, Nishi J, Hashiguchi S, Yamaki Y, Kurauchi K, Tanabe T, Shinkoda Y, Nishikawa T, Suda Y, Kawano Y
  • Journal Title: J Pediatr Hematol Oncol Volume: 35(5) Pages: e224-225
  • Peer Reviewed
• [Journal Article] Prospective safety monitoring of Haemophilus influenzae type b and heptavalent pneumococcal conjugate vaccines in Kagoshima, Japan (2013)
  • Author(s): Nishi J, Tokuda K, Imuta N, Minami T, Kawano Y
  • Journal Title: Jpn J Infect Dis Volume: 66(3) Pages: 235-237
  • Peer Reviewed
• [Journal Article] the Japanese IPD Study Group. Low opsonic activity to the infecting serotype in pediatric patients with invasive pneumococcal disease (2013)
  • Author(s): Oishi T, Ishiwada N, Matsubara K, Nishi J, Chang B, Tamura K, Akeda Y, Ihara T, Nahm MH, Oishi K
  • Journal Title: Vaccine Volume: 31(5) Pages: 845-849
  • Peer Reviewed
• [Journal Article] Electrocardiographic screening of 1-month-old infants for identifying prolonged QT intervals (2013)
  • Author(s): Yoshinaga M, Ushinohama H, Sato S, Tauchi N, Horigome H, Takahashi H, Sumitomo N, Kucho Y, Shiraishi H, Nomura Y, Shimizu W, Nagashima M
  • Journal Title: Circ Arrhythm Electrophysiol Volume: 6(5) Pages: 932-938
  • Peer Reviewed
• [Journal Article] Novel cholix toxin variants, ADP-ribosylating toxins in Vibrio cholerae non-O1/non-O139 strains, and their pathogenicity (2013)
  • Author(s): Awasthi SP, Asakura M, Chowdhury N, Neogi SB, Hinenoya A, Golbar HM, Yamate J, Arakawa E, Tada T, Ramamurthy T, Yamasaki S
  • Journal Title: Infect Immun Volume: 81(2) Pages: 531-541
  • Peer Reviewed
• [Journal Article] Reduction in the incidence of MRSA with use of alcohol-based hand rub solutions and gloves (2012)
  • Author(s): Matsumoto K, Shigemi A, Yaji K, Shimodozono Y, Takeda Y, Ikawa K, Morikawa N, Miyanohara H, Kawamura J, Orita M, Tokuda K, Nishi J, Yamada K
  • Journal Title: J Infect Chemother Volume: 18(2) Pages: 269-271
  • Peer Reviewed
• [Journal Article] Potential role of autoantibody in severe neutropenia of a patient with Kawasaki Syndrome (2012)
  • Author(s): Ueno K, Nomura Y, Masamoto I, Masuda K, Morita Y, Eguchi T, Okamoto Y, Kawano Y
  • Journal Title: Scand J Immunol Volume: 75(1) Pages: 120-126
  • Peer Reviewed
• [Journal Article] Serum procalcitonin value is useful for predicting severity of Kawasaki disease (2012)
  • Author(s): Yoshikawa H, Nomura Y, Masuda K, Koriya C, Arata M, Hazeki D, Yanagimoto K, Ueno K, Eguchi T, Kawano Y
  • Journal Title: Pediatr Infect Dis J Volume: 31(5) Pages: 523-525
  • Peer Reviewed
• [Journal Article] Kawasaki disease patients with six principal symptoms have a high risk of being a non-responder (2012)
  • Author(s): Nomura Y, Arata M, Masuda K, Koriyama C, Suruki N, Ueno K, Yoshikawa H, Eguchi T, Kawano Y
  • Journal Title: Pediatr Int Volume: 54(1) Pages: 14-18
  • Peer Reviewed
• [Journal Article] Prevalence of Providencia strains among children with diarrhea in Japan (2012)
  • Author(s): Shima A, Hinenoya A, Asakura M, Nagita A, Yamasaki S
  • Journal Title: Jpn J Infect Dis Volume: 65(6) Pages: 545-547
  • Peer Reviewed
• [Journal Article] Molecular characterizations of cytolethal distending toxin produced by Providencia alcalifaciens strains isolated from patients with diarrhea (2012)
  • Author(s): Shima A, Hinenoya A, Asakura M, Sugimoto N, Tsukamoto T, Ito H, Nagita A, Faruque SM, Yamasaki S
  • Journal Title: Infect Immun Volume: 80(4) Pages: 1323-1332
  • Peer Reviewed
• [Journal Article] A cytolethal distending toxin gene-based multiplex PCR assay for detection of Campylobacter spp. in stool specimens and comparison with culture method (2012)
  • Author(s): Shiramaru S, Asakura M, Inoue H, Nagita A, Matsuhisa A, Yamasaki S
  • Journal Title: J Vet Med Sci Volume: 74(7) Pages: 857-862
  • Peer Reviewed
• [Journal Article] Quantitative analysis of biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA) strains from patients with orthopaedic device-related infections (2011)
  • Author(s): Kawamura H, Nishi J, Imuta N, Tokuda K, Miyanohara H, Hashiguchi T, Zenmyo M, Yamamoto T, Ijiri K, Kawano Y, Komiya S
  • Journal Title: FEMS Immunol Med Microbiol Volume: 63(1) Pages: 10-15
  • Peer Reviewed
• [Journal Article] Environmental mutagens may be implicated in the emergence of drug-resistant microorganisms (2011)
  • Author(s): Miyahara E, Nishie M, Takumi S, Miyanohara H, Nishi J, Yoshiie K, Oda H, Takeuchi M, Komatsu M, Aoyama K, Horiuchi M, Takeuchi T
  • Journal Title: FEMS Microbiol Lett Volume: 317(2) Pages: 109-116
  • Peer Reviewed
• [Journal Article] Development of Kawasaki syndrome in autoimmune neutropenia after treatment with granulocyte colony-stimulating factor (2011)
  • Author(s): Ueno K, Nomura Y, Arata M, Maruyama S, Tanabe T, Eguchi T, Kawano Y
  • Journal Title: Pediatr Int Volume: 53(3) Pages: 388-390
  • Peer Reviewed
• [Journal Article] Identification of Vibrio campbellii isolated from diseased farm-shrimps from south India and establishment of its pathogenic potential in an Artemia model (2011)
  • Author(s): Haldar S, Chatterjee S, Sugimoto N, Das S, Chowdhury N, Hinenoya A, Asakura M, Yamasaki S
  • Journal Title: Microbiology Volume: 157(Pt 1) Pages: 179-188
  • Peer Reviewed
• [Journal Article] Evaluation of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the identification of Campylobacter strains isolated from diarrheal patients in Japan (2011)
  • Author(s): Kabir SM, Kikuchi K, Asakura M, Shiramaru S, Tsuruoka N, Goto A, Hinenoya A, Yamasaki S
  • Journal Title: Jpn J Infect Dis Volume: 64(1) Pages: 19-27
  • Peer Reviewed
• [Journal Article] Inhibition of virulence potential of Vibrio cholerae by natural compounds (2011)
  • Author(s): Yamasaki S, Asakura M, Neogi SB, Hinenoya A, Iwaoka E, Aoki S
  • Journal Title: Indian J Med Res Volume: 133 Pages: 232-239
  • Peer Reviewed
• [Presentation] Molecular epidemiological analysis of extended-spectrum beta-lactamase (ESBL)-producing enteroaggregative Escherichia coli (EAEC) (2014)
  • Author(s): Nishi J, Imuta N, Tokuda K, Yoshiie K
  • Organizer: The 114th General Meeting of the American Society for Microbiology
  • Place of Presentation: Boston, USA
  • Year and Date: 20140517-20
• [Presentation] Effects of Care Bundle for Preventing Orthopedic Methicillin-resistant Staphylococcus aureus (MRSA) Surgical Site Infections (SSI) (2013)
  • Author(s): Kawamura H, Shigemi A, Koriyama T, Matsumoto K, Orita M, Komiya S, Tokuda K, Nishi J
  • Organizer: ID Week 2013
  • Place of Presentation: San Francisco, USA
  • Year and Date: 20131002-06
• [Presentation] High prevalence of afimbrial adhesin gene among ESBL producing EAEC isolates (2013)
  • Author(s): Imuta N, Yoshiie K, Matayoshi S, Nishi J
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉
  • Year and Date: 20130318-20
• [Presentation] Mixed culture of Escherichia coli strains easily produce drug resistant population (2013)
  • Author(s): Yoshiie K, Imuta N, Matayoshi S, Nishi J
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉
  • Year and Date: 20130318-20
• [Presentation] 過去10年間の小児下痢症患児由来大腸菌におけるESBL産生菌の推移-腸管凝集性大腸菌の薬剤耐性化- (2012)
  • Author(s): 藺牟田直子,郡山豊泰,徳田浩一,又吉盛健,吉家清貴,西順一郎
  • Organizer: 第82回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 20121105-07
• [Presentation] Usefulness of phage open-reading frame typing method for the epidemiological analysis of nosocomial methicillin-resistant Staphylococcus aureus outbreaks in a tertiary care hospital in Japan (2012)
  • Author(s): Kawamura H, Tokuda K, Imuta N, Miyanohara H, Koriyama T, Kawano Y, Komiya S, Nishi J
  • Organizer: ID Week 2012
  • Place of Presentation: San Diego, USA
  • Year and Date: 20121017-21
• [Presentation] 鹿児島大学病院における過去3年間のESBL産生菌の遺伝子型解析と薬剤感受性試験 (2012)
  • Author(s): 郡山豊泰,松元一明,川村英樹,西順一郎
  • Organizer: 第86回日本感染症学会総会
  • Place of Presentation: 長崎
  • Year and Date: 20120425-26
• [Presentation] 小児下痢症患者由来の大腸菌におけるESBL(基質拡張型βラクタマーゼ)産生菌の年次推移 (2012)
  • Author(s): 藺牟田直子,郡山豊泰,川村秀樹,西順一郎
  • Organizer: 第86回日本感染症学会総会
  • Place of Presentation: 長崎
  • Year and Date: 20120425-26
• [Presentation] High prevalence of ESBL genes among enteroaggregative Escherichia coli (EAEC) isolates in Kagoshima, Japan (2012)
  • Author(s): Imuta N, Nishi J
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 20120327-29
• [Presentation] ESBL(基質拡張型βラクタマーゼ)を産生する腸管凝集性大腸菌の薬剤耐性状況 (2012)
  • Author(s): 藺牟田直子,徳田浩一,河野嘉文,西順一郎
  • Organizer: 第44回日本小児感染症学会総会・学術集会
  • Place of Presentation: 北九州
  • Year and Date: 2012-11-24
• [Presentation] ESBL産生腸管凝集性大腸菌はafimbrial adhesin I遺伝子(afaI)を高頻度に保有する (2012)
  • Author(s): 藺牟田直子,吉家清貴,又吉盛健,西順一郎
  • Organizer: 第65回日本細菌学会九州支部総会
  • Place of Presentation: 那覇
  • Year and Date: 2012-08-25
• [Presentation] ESBLを産生する腸管凝集性大腸菌の検出頻度と薬剤耐性状況 (2012)
  • Author(s): 藺牟田直子,郡山豊泰,徳田浩一,西順一郎
  • Organizer: 第16回腸管出血性大腸菌感染症研究会
  • Place of Presentation: 秋田
  • Year and Date: 2012-07-19
• [Presentation] 腸管凝集性大腸菌はESBL (基質拡張型βラクタマーゼ)遺伝子を高頻度に保有する (2012)
  • Author(s): 藺牟田直子,西順一郎
  • Organizer: 第8回日本小児消化管感染症研究会
  • Place of Presentation: 東京
  • Year and Date: 2012-02-11
• [Presentation] 小児下痢症患児から分離された大腸菌におけるcytolethal distending toxin (CDT)遺伝子保有状況 (2011)
  • Author(s): 藺牟田直子,西順一郎,徳田浩一,河野嘉文
  • Organizer: 第43回日本小児感染症学会
  • Place of Presentation: 岡山市
  • Year and Date: 20111029-30
• [Presentation] 腸管出血性大腸菌サーベイランスにおける地理情報システム(GIS)を用いた効果的情報還元に関する研究 (2011)
  • Author(s): 徳田浩一,西順一郎,藺牟田直子,河野嘉文
  • Organizer: 第43回日本小児感染症学会
  • Place of Presentation: 岡山市
  • Year and Date: 20111029-30
• [Presentation] 小児下痢症患児から分離された大腸菌におけるESBL(基質拡張型βラクタマーゼ)遺伝子の保有状況 (2011)
  • Author(s): 西順一郎,藺牟田直子,河野嘉文
  • Organizer: 第43回日本小児感染症学会
  • Place of Presentation: 岡山市
  • Year and Date: 20111029-30
• [Presentation] 小児下痢症患児由来大腸菌における病原遺伝子とβ-ラクタマーゼ遺伝子の分布 (2011)
  • Author(s): 西順一郎,藺牟田直子,郡山豊泰
  • Organizer: 第81回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 北九州市
  • Year and Date: 20111006-08
• [Presentation] 鹿児島大学病院における過去3年間の基質拡張型β-ラクタマーゼ(ESBL)産生菌の検出状況 (2011)
  • Author(s): 郡山豊泰,松元一明,川村英樹,西順一郎
  • Organizer: 第81回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 北九州市
  • Year and Date: 20111006-08
• [Presentation] Prevalence of dispersin (Aap) among diarrheagenic Esherichia coli isolated from children with diarrhea in Kagoshima, Japan (2011)
  • Author(s): Nishi J, Imuta N
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
  • Year and Date: 20110906-10
• [Presentation] Prevalence of cytolethal distending toxin (CDT) gene among Escherichia coli isolates from children with diarrhea in Kagoshima, Japan (2011)
  • Author(s): Imuta N, Nishi J
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo(国内学会)
  • Year and Date: 20110906-10
• [Presentation] 小児下痢症患児由来の大腸菌におけるESBL(基質拡張型βラクタマーゼ)遺伝子の分布 (2011)
  • Author(s): 西順一郎,藺牟田直子
  • Organizer: 第15回腸管出血性大腸菌感染症研究会
  • Place of Presentation: 大阪
  • Year and Date: 20110715-16
• [Presentation] 小児の腸管出血性大腸菌感染におけるHUSおよび脳症合併に関連する因子の検討 (2011)
  • Author(s): 田尻仁,西順一郎,山元公恵,牛島高介,高野智子
  • Organizer: 第15回腸管出血性大腸菌感染症研究会
  • Place of Presentation: 大阪
  • Year and Date: 20110715-16
• [Presentation] 小児下痢症患児由来大腸菌における下痢原性大腸菌とEBL(基質拡張型βラクタマーゼ)産生菌の分布 (2011)
  • Author(s): 西順一郎,藺牟田直子,郡山豊泰,川村英樹
  • Organizer: 第64回日本細菌学会九州支部総会
  • Place of Presentation: 北九州
  • Year and Date: 2011-08-26
• [Remarks]
  • URL: http://www.kufm.kagoshima-u.ac.jp/~bacterio/index.html