2014 Fiscal Year Final Research Report
Neuroprotective treatment of therombomodulin for severe neonatal asphyxia in rats
| Project/Area Number |
23591599
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Research Collaborator |
ARAI Ikuyo 奈良県立医科大学
ITOU Akito 日精バイリス株式会社, 滋賀研究所
SAKONJOU Hiroshi 日精バイリス株式会社, 滋賀研究所
KAJIKI Masahiro 旭化成ファーマ株式会社
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Keywords | 新生児仮死 / 低酸素性虚血性脳症 / トロンボモジュリン / 神経保護作用 |
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| Outline of Final Research Achievements |
This study aimed to evaluate the neuroprotective effects of thrombomodulin during middle cerebral artery occlusion (MCAO) in rat stroke model. Sprague Dawley (SD) rats (N =10) were randomly divided into two groups: control (N = 5), and treatment (N = 5) groups. The intraluminal monofilament was inserted right middle cerebral artery by the surgical procedure, and it was occulted permanently. The treatment group received intravenous thromobomodulin injections, and the control group received normal saline injections. After six hours, The infarct of brain slice generated by MCAO was stained in 2% 2,3,5-triphenyltetrazolium chloride (TTC). The size and extent of the infarcted area was determined using NIH Image J/Fiji 1.46 . Neuroprotective effect was observed significantly in the cortex area but not in the striate area. The data indicate that thrombomoduline has the neuroprotective effects on MCAO rat stroke model, which needs further studies for confirmation.
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| Free Research Field |
新生児の止血機構
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