The elucidation of the mechanism of growth suppression of melanoma by kinase inhibitor

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591614
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: University of Yamanashi
• Principal Investigator: HARADA Kazutoshi 山梨大学, 医学部附属病院, 講師 (20324197)
• Project Period (FY): 2011 – 2013
• Keywords: キナーゼ阻害薬 / シグナル伝達系 / 悪性黒色腫

Research Abstract

Wnt sinaling pathway has a crucial role in tumorgenesis. Some report have demonstrated that this signaling pathway was activated in melanoma. In this study, we evaluated whether celecoxib, a NSAIDs inhibited Wnt signaling pathway or not. If celecoxib suppressed Wnt signaling, this drug can be used as a molecular targeting drug. The expression of beta-catenin in melanoma samples was assessed by immunohistochemistry. Some melanoma samples expressed beta-catenin. Next, celecoxib was administrated in cell culture medium of melanoma cells. This experiment revealed that celecoxib inhibited the growth of melanoma cells. The relationship between the amount of beta-catenin in melanoma cells and the extent of growth suppression of melanoma cells was investigated, suggesting correlation of Wnt signal activation and the effect of growth inhibition of melanoma cells.

Research Products

• [Journal Article] Case of primary cutaneous peripheral T-cell lymphoma, not otherwise specified, with characteristics of follicular helper T cells (2014)
  • Author(s): Takaki M, Inozume T, Matsuzawa T, Ando N, Yamaguchi M, Harada K, Kawamura T, Shibagaki N, Shimada S
  • Journal Title: J Dermatol
  • DOI: 10.1111/1346-8138.12493
  • Peer Reviewed
• [Journal Article] Nodular melanoma on the hyponychium : clinical and dermoscopic features (2014)
  • Author(s): Yamamoto S, Harada K, Ando N, Kawamura T, Shibagaki N, Tanaka M, Shimada S
  • Journal Title: J Dermatol Volume: 41(3)(PubMed PMID:24765666) Pages: 277-8
  • Peer Reviewed
• [Journal Article] CD271 on Melanoma Cell Is an IFN-γ-Inducible Immunosuppressive Factor that Mediates Downregulation of Melanoma Antigens (2014)
  • Author(s): Furuta J, Inozume T, Harada K, Shimada S
  • Journal Title: J Invest Dermatol Volume: 134(5)(Epub 2013 Nov 13. PubMed PMID:24226422) Pages: 1369-77
  • DOI: 10.1038/jid.2013.490
  • Peer Reviewed
• [Journal Article] Two cases of autosomal recessive woolly hair with LIPH gene mutations (2013)
  • Author(s): Harada K, Inozume T, Kawamura T, Shibagaki N, Kinoshita T, Deguchi N, Shimada S
  • Journal Title: Int J Dermatol Volume: 52(5)(PubMed PMID:23590372) Pages: 572-4
  • DOI: 10.1111/j.1365-4632.2012.05775.x
  • Peer Reviewed
• [Journal Article] Growth retardation and hair loss in transgenic mice overexpressing human H-ferritin gene (2013)
  • Author(s): Hasegawa S, Harada K, Morokoshi Y, Tsukamoto S, Furukawa T, Saga T
  • Journal Title: Transgenic Res Volume: 22(3)(Epub 2012 Oct 31. PubMed PMID:23111618) Pages: 651-8
  • DOI: 10.1007/s11248-012-9669-0
  • Peer Reviewed
• [Journal Article] Mutation analysis of BRAF and KIT in circulating melanoma cells at the single cell level (2012)
  • Author(s): Sakaizawa K, Goto Y, Kiniwa Y, Uchiyama A, Harada K, Shimada S, Saida T, Ferrone S, Takata M, Uhara H, Okuyama R
  • Journal Title: Br J Cancer Volume: 106(5)(Epub 2012 Jan 26. PubMed PMID:22281663; PubMed Central PMCID:PMC3305957) Pages: 939-46
  • DOI: 10.1038/bjc.2012.12
  • Peer Reviewed
• [Journal Article] A patient with pseudochromhidrosis presenting with pink nails (2012)
  • Author(s): Harada K, Morohoshi T, Ikeda T, Shimada S
  • Journal Title: J Am Acad Dermatol Volume: 67(2)(PubMed PMID:22794820) Pages: e74-5
  • DOI: 10.1016/j.jaad.2011.08.026
  • Peer Reviewed
• [Journal Article] Marked response to imatinib mesylate in metastatic acral lentiginous melanoma on the thumb (2011)
  • Author(s): Yamaguchi M, Harada K, Ando N, Kawamura T, Shibagaki N, Shimada S
  • Journal Title: Clin Exp Dermatol Volume: 36(2)(PubMed PMID:20545949) Pages: 174-7
  • DOI: 10.1111/j.1365-2230.2010.03885.x
  • Peer Reviewed
• [Remarks]
  • URL: http://www.med.yamanashi.ac.jp/clinical/dermatol/index.html