Transcription factors Runx regulates differentiation and proliferation of keratinocyte

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591615
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Shinshu University
• Principal Investigator: OKUYAMA Ryuhei 信州大学, 医学部, 教授 (80292332)
• Co-Investigator(Renkei-kenkyūsha): IKAWA Shuntaro 東北大学, 加齢医学研究所, 准教授 (50241576) ; SATAKE Masanobu 東北大学, 加齢医学研究所, 教授 (50178688)
• Project Period (FY): 2011 – 2013
• Keywords: Runx / 表皮 / 増殖 / 分化

Research Abstract

Runt-related transciption factor (Runx) family is implicated in stem cell regulation, tissue development and oncogenesis. Runx consists of Runx1~3 and plays important roles in several organs by affecting cell survival, proliferation and differentiation. However, it is unknown whether and how Runx regulates in the epidermis. We investigated the functions of Runx1 and Runx3 in the epidermis.
We overexpressed Runx1 and Runx3 in primary mouse keratinocytes by using adenovirus vectors. Furthermore, we decreased the expression of Runx1 and Runx3 by using sequence-specific siRNAs. These experiments showed that Runx inhibits both differentiation and proliferation in keratinocyte.

Research Products

• [Journal Article] Histopathological correlation to the dermoscopic feature of"string of pearls"in clear cell acanthoma (2014)
  • Author(s): Miyake T, Minagawa A, Koga H, Fukuzawa M, Okuyama R
  • Journal Title: Eur J Dermatol
  • Peer Reviewed
• [Journal Article] A Case of Kindler Syndrome with Severe Esophageal Stenosis (2014)
  • Author(s): Ohashi A, Kiniwa Y, Kosho T, Suga T, Has C, Kubo H, Okuyama R
  • Journal Title: Int J Dermatol
  • Peer Reviewed
• [Journal Article] Alteration of dermoscopic features in a juvenile xanthogranuloma during follow-up of 43 months (2014)
  • Author(s): Unno T, Minagawa A, Koga H, Uhara H, Okuyama R
  • Journal Title: Int J Dermatol
  • Peer Reviewed
• [Journal Article] NRAS mutations in primary and metastatic melanomas of Japanese patients (2014)
  • Author(s): Uhara H, Ashida A, Koga H, Uchiyama A, Uchiyama R, Hayashi K, Kiniwa Y, Okuyama R
  • Journal Title: Int J Clin Oncol
  • Peer Reviewed
• [Journal Article] Coexpression of EpCAM, CD44 variant isoforms and Claudin-7 in anaplastic thyroid carcinoma (2014)
  • Author(s): Okada T, Nakamura T, Watanabe T, Onoda N, Asida A, Okuyama R, Ito K
  • Journal Title: PLoS One Volume: 9 Pages: e94487
  • DOI: 10.1371/journal.pone.0094487
  • Peer Reviewed
• [Journal Article] Acral melanocytic nevus showing parallel ridge pattern due to predominant distribution of melanin column under the surface ridge (2014)
  • Author(s): Sano T, Minigawa A, Koga H, Uhara H, Okuyama R
  • Journal Title: Acta Derm Venereol
  • DOI: 10.2340/00015555-1865
  • Peer Reviewed
• [Journal Article] Generalized pustulosis associated with disseminated Mycobacterium avium infection in a non-AIDS patient (2014)
  • Author(s): Asai Y, Kiniwa Y, Okada N, Futatsugi T, Ushiki A, Uhara H, Okuyama R
  • Journal Title: Eur J Dermatol
  • DOI: 10.2340/00015555-1836
  • Peer Reviewed
• [Journal Article] IgA/IgG pemphigus with infiltration of neutrophils and eosinophils in an ulcerative colitis patient (2014)
  • Author(s): Uchiyama R, Ishii N, Arakura F, Kiniwa Y, Nakazawa K, Uhara H, Hashimoto T, Okuyama R
  • Journal Title: Acta Derm Venereol
  • DOI: 10.2340/00015555-1836
  • Peer Reviewed
• [Journal Article] Vascular structures absence under dermoscopy in two cases of Angiosarcoma on the scalp (2014)
  • Author(s): Minagawa A, Koga H, Okuyama R
  • Journal Title: Int J Dermatol
  • DOI: 10.1111/ijd.12357
  • Peer Reviewed
• [Journal Article] Marked histiocytic infiltration in neonatal lupus erythematosus (2014)
  • Author(s): Okada N, Okuyama R, Uhara H
  • Journal Title: J Dermatol Volume: 41 Pages: 192-193
  • DOI: 10.1111/1346-8138.12381
  • Peer Reviewed
• [Journal Article] Successful treatment of rheumatoid vasculitis-associated skin ulcer with a TNFαantagonist (2014)
  • Author(s): Ashida A, Murata H, Mikoshiba Y, Ohashi A, Kobayashi A, Koga H, Uhara H, Okuyama R
  • Journal Title: Int J Dermatol Volume: 53 Pages: e154-156
  • DOI: 10.1111/j.1365-4632.2012.05529.x
  • Peer Reviewed
• [Journal Article] Usefulness of high-frequency sonography for the diagnosis of asymptomatic myopathy in Löfgren's syndrome (2014)
  • Author(s): Ohashi A, Koga H, Hayashi K, Uhara H, Okuyama R
  • Journal Title: Int J Dermatol Volume: 53 Pages: e62-63
  • DOI: 10.1111/j.1365-4632.2012.05489.x
  • Peer Reviewed
• [Journal Article] Unilateral pigmentary purpuric dermatosis associated with deep thrombosis (2014)
  • Author(s): Mikoshiba Y, Saiki M, Uhara H, Okuyama R
  • Journal Title: Int J Dermatol Volume: 53 Pages: e87-88
  • DOI: 10.1111/j.1365-4632.2012.05492.x
  • Peer Reviewed
• [Journal Article] Generalised acanthosis nigricans in childhood with short stature associated with poor response to growth hormone provocation (2014)
  • Author(s): Iwade M, Ogawa E, Koga H, Mizuno F, Koike K, Matsumoto K, Okuyama R
  • Journal Title: Acta Derm Venereol
  • DOI: 10.2340/00015555-1759
  • Peer Reviewed
• [Journal Article] 5-hydroxymethylcytosine as a useful marker to differentiate between malignant melanomas and benign melanocytic nevi (2014)
  • Author(s): Uchiyama R, Uhar H, Uchiyama A, Ogawa E, Takazawa Y, Ashida A, Koga H, Hayashi K, Kiniwa Y, Okuyama R
  • Journal Title: J Dermatol Sci Volume: 73 Pages: 161-163
  • DOI: 10.1016/j.jdermsci.2013.09.008
  • Peer Reviewed
• [Journal Article] 抗CTLA-4抗体(ipilimumab)を用いたメラノーマ治療 (2014)
  • Author(s): 木庭幸子、奥山隆平
  • Journal Title: がん分子標的治療12 Volume: 1 Pages: 23-26
• [Journal Article] Combination chemotherapy of carboplatin and paclitaxel for metastatic melanoma (2013)
  • Author(s): Sato Y, Uhara H, Ashida A, Okuyama R
  • Journal Title: J Dermatol Volume: 40 Pages: 1050-1051
  • DOI: 10.1111/1346-8138.12306
  • Peer Reviewed
• [Journal Article] Lamellar ichthyosis in a collodion baby caused by CYP4F22 mutations in a non-consanguineous family outside the Mediterranean (2013)
  • Author(s): Sugiura K, Takeichi T, Ito Y, Kosho T, Uhara H, Okuyama R, Akiyama M
  • Journal Title: J Dermatol Sci Volume: 72 Pages: 193-195
  • DOI: 10.1016/j.jdermsci.2013.06.008
  • Peer Reviewed
• [Journal Article] Clinical effects of stereotactic radiation surgery in patients with metastatic melanoma (2013)
  • Author(s): Mikoshiba A, Uhara H, Murata H, Okuyama R
  • Journal Title: J Dermatol Volume: 40 Pages: 626-628
  • DOI: 10.1111/1346-8138.12179
  • Peer Reviewed
• [Journal Article] S-1 induced a durable response in metastatic extramammary Paget's disease (2013)
  • Author(s): Mikoshiba Y, Uhara H, Kubo H, Okuyama R
  • Journal Title: J Dermatol Volume: 40 Pages: 664-665
  • DOI: 10.1111/1346-8138.12177
  • Peer Reviewed
• [Journal Article] A case of azacitidine-induced maculopapular erythematous eruption (2013)
  • Author(s): Ohashi A, Hayashi K, Okuyama R
  • Journal Title: J Dermatol Volume: 40 Pages: 680-681
  • DOI: 10.1111/1346-8138.12181
  • Peer Reviewed
• [Journal Article] Age-related prevalence of dermoscopic patterns in acquired melanocytic nevus on acral volar skin (2013)
  • Author(s): Minagawa A, Koga H, Uhara H, Yoshiharu Yokokawa Okuyama R
  • Journal Title: JAMA Dermatol Volume: 149 Pages: 989-990
  • DOI: 10.1001/jamadermatol.2013.4452
  • Peer Reviewed
• [Journal Article] Dermoscopic characteristics of acquired melanocytic nevus in childhood affecting on acral region (2013)
  • Author(s): Minagawa A, Koga H, Uhara H, Okuyama R
  • Journal Title: Acta Derm Venereol Volume: 93 Pages: 751-752
  • DOI: 10.2340/00015555-1587
  • Peer Reviewed
• [Journal Article] Opposing functions of Fbxw7 in keratinocyte growth-differentiation and skin tumorigesis mediated through negative regulation of c-Myc and Notch (2013)
  • Author(s): Ishikawa Y, Hosogane M, Okuyama R, Aoyama S, Onoyama I, Nakayama K, Nakayama K
  • Journal Title: Oncogene Volume: 32 Pages: 1921-1932
  • DOI: 10.1038/onc.2012.213
  • Peer Reviewed
• [Journal Article] Effectiveness of topical maxacalcitol for acquired perforating disorder (2013)
  • Author(s): Yoshiya H, Okuyama R, Uhara H
  • Journal Title: J Am Acad Dermatol Volume: 68 Pages: e181-2
  • DOI: 10.1016/j.jaad.2012.10.044
  • Peer Reviewed
• [Journal Article] Low p53 positivity in verrucous skin lesion in diabetic neuropathy occurring on the dorsum of the foot (2013)
  • Author(s): Ashida A, Kiniwa Y, Kobayashi A, Matsumoto K, Okuyama R
  • Journal Title: Int J Dermatol Volume: 52 Pages: 378-380
  • DOI: 10.1111/j.1365-4632.2011.04891.x
  • Peer Reviewed
• [Journal Article] Clinical courses of drug-induced hypersensitivity syndrome treated without systemic corticosteroids (2013)
  • Author(s): Uhara H, Saiki M, Kawachi S, Ashida A, Oguchi S, Okuyama R
  • Journal Title: J Eur Acad Derm Venereol Volume: 27 Pages: 722-726
  • DOI: 10.1111/j.1468-3083.2012.04547.x
  • Peer Reviewed
• [Journal Article] Fibrosarcoma variant of dermatofibrosarcoma protuberans with pancreatic metastasis (2013)
  • Author(s): Murata H, Ohashi A, Ashida A, Uhara H, Okuyama R, Nakata T, Shingu K
  • Journal Title: Int J Dermatol Volume: 53 Pages: e140-2
  • DOI: 10.1111/j.1365-4632.2012.05698.x
  • Peer Reviewed
• [Journal Article] Platinum and anthracycline therapy for advanced cutaneous squamous cell carcinoma (2013)
  • Author(s): Nakamura K, Okuyama R, Saida T, Uhara H
  • Journal Title: Int J Clin Oncol Volume: 18 Pages: 506-9
  • DOI: 10.1007/s10147-012-0411-y
  • Peer Reviewed
• [Journal Article] Bullous formation in a patient with familiar amyloid polyneuropathy type I (2013)
  • Author(s): Kobayashi A, Uhara H, Kido K, Sekijima Y, Tojo K, Ikeda S, Okuyama R
  • Journal Title: Int J Dermatol Volume: 52 Pages: 1398-1400
  • DOI: 10.1111/j.1365-4632.2011.05146.x
  • Peer Reviewed
• [Journal Article] A case of hypocomplementemic urticarial vasculitis with a high serum level of rheumatoid factor (2013)
  • Author(s): Ashida A, Murata H, Ohashi A, Ogawa E, Uhara H, Okuyama R
  • Journal Title: Australas J Dermatol Volume: 54 Pages: e62-e63
  • DOI: 10.1111/j.1440-0960.2011.00873.x
  • Peer Reviewed
• [Journal Article] 基底細胞癌におけるmiRNAの意義 (2013)
  • Author(s): 岡田なぎさ、木庭幸子、宇原久、奥山隆平
  • Journal Title: 日本臨床 Volume: 71増刊号4 Pages: 587-592
• [Journal Article] 悪性黒色腫とエピジェネティクス (2013)
  • Author(s): 奥山隆平、宇原久
  • Journal Title: 日本臨床 Volume: 71増刊号4 Pages: 161-165
• [Journal Article] Genome wide association studyの知見 (2013)
  • Author(s): 奥山隆平、宇原久
  • Journal Title: 日本臨床 Volume: 71増刊号4 Pages: 157-160
• [Journal Article] 転写因子MITFの作用 (2013)
  • Author(s): 木藤健治、宇原久、奥山隆平
  • Journal Title: 日本臨床 Volume: 71増刊号4 Pages: 149-153
• [Journal Article] MAPキナーゼ:NRAS、BRAF、MEK (2013)
  • Author(s): 芦田敦子、木庭幸子、宇原久、奥山隆平
  • Journal Title: 日本臨床 Volume: 71増刊号4 Pages: 126-129
• [Journal Article] 悪性黒色腫におけるエクソソームの意義 (2013)
  • Author(s): 奥山隆平、宇原久
  • Journal Title: 日本臨床 Volume: 71増刊号4 Pages: 98-102
• [Journal Article] 悪性黒色腫の循環腫瘍細胞 (2013)
  • Author(s): 上條史尚、木庭幸子、宇原久、奥山隆平
  • Journal Title: 日本臨床 Volume: 71増刊号4 Pages: 93-97
• [Journal Article] 長野県における乾癬治療の現状と病診連携の可能性 (2013)
  • Author(s): 小林彩、吉川美香、小川英作、奥山隆平
  • Journal Title: 西日本皮膚科 Volume: 75 Pages: 346-349
• [Journal Article] Epidermal-type FABP is a predictive marker of clinical response to systemic treatment and ultraviolet therapy in psoriatic skin lesions (2012)
  • Author(s): Miyake T, Ogawa E, Mikoshiba A, Kobayashi A, Hosoe H, Kashiwabara S, Uhara H, Owada Y, Okuyama R
  • Journal Title: J Dermatol Sci Volume: 68 Pages: 199-202
  • DOI: 10.1016/j.jdermsci.2012.09.002
  • Peer Reviewed
• [Journal Article] Reduced size of sebaceous gland and altered sebum lipid composition in mice deficient in the fatty acid-binding protein 5 gene (2012)
  • Author(s): Sugawara T, Nemoto K, Adachi Y, Yamano N, Tokuda N, Muto M, Okuyama R, Sakai S, Owada Y
  • Journal Title: Exp Dermatol Volume: 21 Pages: 543-6
  • DOI: 10.1111/j.1600-0625.2012.01514.x
  • Peer Reviewed
• [Journal Article] Assessment of BRAF and KIT mutations in Japanese melanoma patients (2012)
  • Author(s): Ashida A, Uhara H, Kiniwa Y, Oguchi M, Murata H, Goto Y, Uchiyama A, Ogawa E, Hayashi K, Koga H, Okuyama R
  • Journal Title: J Dermatol Sci Volume: 66 Pages: 240-242
  • DOI: 10.1016/j.jdermsci.2012.03.005
  • Peer Reviewed
• [Journal Article] Mutation analysis of BRAF and KIT in circulating melanoma cells at the single cell level (2012)
  • Author(s): Sakaizawa K, Goto Y, Kiniwa Y, Uchiyama A, Harada K, Shimada S, Saida T, Ferrone S, Takata M, Uhara H, Okuyama R
  • Journal Title: British J Cancer Volume: 106 Pages: 939-946
  • DOI: 10.1038/bjc.2012.12
  • Peer Reviewed
• [Journal Article] A case of Mycobacterial skin disease caused by Mycobacterium peregrinum, and a review of cutaneous infection (2012)
  • Author(s): Kamijo F, Uhara H, Kubo H, Nakanaga K, Hoshino Y, Ishii N, Okuyama R
  • Journal Title: Case Rep Dermatol Volume: 4 Pages: 76-79
  • DOI: 10.1159/000337825
  • Peer Reviewed
• [Journal Article] Sweat test by water-erasable ink (2012)
  • Author(s): Uhara H, Nakamura K, Mikoshiba Y, Miyake T, Okuyama R
  • Journal Title: Acta Derm Venereol Volume: 92 Pages: 432-433
  • DOI: 10.2340/00015555-1300
  • Peer Reviewed
• [Journal Article] Surgical treatment of nail apparatus melanoma in situ : The use of artificial dermis in reconstruction (2012)
  • Author(s): Hayashi K, Uhara H, Koga H, Okuyama R, Saida T
  • Journal Title: Dermatol Surg Volume: 38 Pages: 692-694
  • DOI: 10.1111/j.1524-4725.2011.02217.x
  • Peer Reviewed
• [Journal Article] Rapid progression of hidradenitis suppurative in the lower leg of a patient with psoriasis vulgaris (2012)
  • Author(s): Tanaka A, Goto Y, Iwade M, Uhara H, Okuyama R
  • Journal Title: Acta Derm Venereol Volume: 92 Pages: 105-106
  • DOI: 10.2340/00015555-1182
  • Peer Reviewed
• [Journal Article] Pregnancy and childbirth in three mothers with recessive dystrophic epidermolysis bullosa (2012)
  • Author(s): Hanafusa T, Tamai K, Umegaki N, Yamaguchi Y, Fukuda S, Yaegashi N, Okuyama R, McGrath JA, Katayama I
  • Journal Title: Clin Exp Dermatol Volume: 37 Pages: 10-14
  • DOI: 10.1111/j.1365-2230.2011.04179.x
  • Peer Reviewed
• [Journal Article] A case of epidermolysis bullosa acquisita associated with laryngeal stenosis (2012)
  • Author(s): Kiniwa Y, Ashida A, Ohashi A, Kitoh R, Fukuda S, Hashimoto T, Okuyama R
  • Journal Title: Acta Derm Venereol Volume: 92 Pages: 93-94
  • DOI: 10.2340/00015555-1163
  • Peer Reviewed
• [Journal Article] Cutaneous angiosarcoma of the leg showing radiation sensitivity (2012)
  • Author(s): Ohashi A, Kubo H, Iwade M, Shiohara J, Takata M, Uhara H, Okuyama R
  • Journal Title: Australas J Dermatol Volume: 53 Pages: e51-53
  • DOI: 10.1111/j.1440-0960.2011.00746.x
  • Peer Reviewed
• [Journal Article] アトピー性皮膚炎 (2012)
  • Author(s): 木庭幸子、奥山隆平
  • Journal Title: 日本臨床 Volume: 70 Pages: 555-560
• [Journal Article] Case of a cutaneous angiomyolipoma in the ear (2012)
  • Author(s): Mikoshiba Y, Murata H, Ashida A, Saito N, Koga H, Uhara H, Okuyama R
  • Journal Title: J Dermatol Volume: 39 Pages: 808-809
  • DOI: 10.1111/j.1346-8138.2011.01426.x
  • Peer Reviewed
• [Journal Article] The open pores with plugs in porokeratosis clearly visualized with the dermoscopic furrow ink test : Report of 3 cases (2011)
  • Author(s): Uhara H, Kamijo F, Okuyama R, Saida T
  • Journal Title: Arch Dermatol Volume: 147 Pages: 866-868
  • DOI: 10.1001/archdermatol.2011.174
  • Peer Reviewed
• [Journal Article] Psoriasiform eruption localized to previously irradiated area (2011)
  • Author(s): Ohashi A, Saiki M, Okuyama R
  • Journal Title: Acta Derm Venereol Volume: 91 Pages: 469
  • DOI: 10.2340/00015555-1058
  • Peer Reviewed
• [Journal Article] Thermal angioedema induced by hot water (2011)
  • Author(s): Kobayashi A, Uhara H, Ashida A, Kiniwa Y, Okuyama R
  • Journal Title: Acta Derm Venereol Volume: 91 Pages: 343-344
  • DOI: 10.2340/00015555-1020
  • Peer Reviewed
• [Journal Article] Leg ulcers associated with positive lupus anticoagulant in two cases of Klinefelter's syndrome (2011)
  • Author(s): Goto Y, Uhara H, Murata H, Koga H, Kosho T, Yamazaki M, Takata M, Okuyama R
  • Journal Title: Acta Derm Venereol Volume: 91 Pages: 90-91
  • DOI: 10.2340/00015555-0957
  • Peer Reviewed
• [Journal Article] Calchiphylaxis with POEMS syndrome (2011)
  • Author(s): Yoshikawa M, Uhara H, Arakura F, Murata H, Kubo H, Takata M, Yoshida K, Okuyama R
  • Journal Title: Acta Derm Venereol Volume: 91 Pages: 98-99
  • DOI: 10.2340/00015555-0968
  • Peer Reviewed
• [Journal Article] Malignant fibrous histiocytoma with in-tansit metastasis (2011)
  • Author(s): Fujimura T, Sugawara M, Haga T, Kariya Y, Okuyama R, Tagami H, Aiba S
  • Journal Title: Case Rep Dermatol Volume: 3 Pages: 164-169
  • DOI: 10.1159/000331324
  • Peer Reviewed
• [Journal Article] Dual odidase 1 (DUOX1) induced by Th2 cytokines promotes STAT6 phosphoylation via oxidative inactivation of PTP1B in human epidermal keratinocytes (2011)
  • Author(s): Hirakawa S, Saito R, Ohara H, Okuyama R, Aiba S
  • Journal Title: J Immunol Volume: 186 Pages: 4762-4770
  • DOI: 10.4049/jimmunol.1000791
  • Peer Reviewed
• [Presentation] A regulation of keratinocyte differentiation and proliferation by transcription factors Runx1 and Runx3 (2013)
  • Author(s): Uchiyama R, Ogawa E, Kiniwa Y, Uhara H, Wong WF, Kofu K, Ikawa S, Satake M, Okuyama R
  • Organizer: International Investigative Dermatology 2013
  • Place of Presentation: Edinburgh, Scotland
  • Year and Date: 20130508-11
• [Book] 完全版マウス・ラット疾患モデル活用ハンドブック (2011)
  • Author(s): 秋山徹、奥山隆平、河府和義編
  • Total Pages: 1-604
  • Publisher: 羊土社