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2013 Fiscal Year Final Research Report

Gene analysis in circulating melanoma cells isolated using novel monoclonal antibodies

Research Project

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Project/Area Number 23591616
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Dermatology
Research InstitutionShinshu University

Principal Investigator

ASHIDA Atsuko  信州大学, 医学部, 助教 (00596786)

Co-Investigator(Kenkyū-buntansha) GOTO Yasufumi  信州大学, 医学部, 非常勤講師 (60467181)
OKUYAMA Ryuhei  信州大学, 医学部, 教授 (80292332)
Project Period (FY) 2011 – 2013
Keywords血液循環腫瘍細胞 / メラノーマ / B7-H3
Research Abstract

We investigated an optimized procedure for the efficient isolation of melanoma cells labeled with HMW-MAA (High-Molecular Weight Melanoma-Associated Antigen) -specific antibodies and/or B7-H3-specific antibodies from the peripheral blood using immunomagnetic beads and fluorescence-activated cell sorting (FACS). Compared with the single-labeling method of melanoma cells using HMW-MAA or B7-H3 antibodies, the double-labeling method using HMW-MAA and B7-H3 antibodies earned high yield.These results suggest that the double-labeling method using HMW-MAA and B7-H3 antibodies is useful for isolating melanoma cells from peripheral blood in patients with melanoma.

  • Research Products

    (11 results)

All 2014 2013 2012

All Journal Article (7 results) (of which Peer Reviewed: 5 results) Presentation (3 results) Book (1 results)

  • [Journal Article] NRAS mutations in primary and metastatic melanomas of Japanese patients2014

    • Author(s)
      Uhara H, Ashida A, Koga H, Uchiyama A, Uchiyama R, Hayashi K, Kiniwa Y, Okuyama R
    • Journal Title

      Int J Clin Oncol

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] 5-hydroxymethylcytosine as a useful marker to differentiate between malignant melanomas and benign melanocytic nevi2014

    • Author(s)
      Uchiyama R, Uhara H, Uchiyama A, Ogawa E, Takazawa Y, Ashida A, Koga H, Hayashi K, Kiniwa Y, Okuyama R
    • Journal Title

      J Dermatol Sci

      Volume: 73 Pages: 161-163

    • Peer Reviewed
  • [Journal Article] Combination chemotherapy of carboplatin and paclitaxel for metastatic melanoma2013

    • Author(s)
      Sato Y, Uhara H, Ashida A, Okuyama R
    • Journal Title

      J Dermatol

      Volume: 40 Pages: 1050-1051

    • Peer Reviewed
  • [Journal Article] チロシンキナーゼ:KIT2013

    • Author(s)
      芦田敦子、木庭幸子、宇原久、奥山隆平
    • Journal Title

      日本臨床

      Volume: 71増刊号4 Pages: 134-138

  • [Journal Article] MAPキナーゼ:NRAS、BRAF、MEK2013

    • Author(s)
      芦田敦子、木庭幸子、宇原久、奥山隆平
    • Journal Title

      日本臨床

      Volume: 71増刊号4 Pages: 126-129

  • [Journal Article] Assessment of BRAF and KIT mutations in Japanese melanoma patients2012

    • Author(s)
      Ashida A, Uhara H, Kiniwa Y, Oguchi M, Murata H, Goto Y, Uchiyama A, Ogawa E, Hayashi K, Koga H, Okuyama R
    • Journal Title

      J Dermatol Sci

      Volume: 66 Pages: 240-242

    • Peer Reviewed
  • [Journal Article] Mutation analysis of BRAF and KIT in circulating melanoma cells at the single cell level2012

    • Author(s)
      SakaizawaK, Goto Y, Kiniwa Y, Uchiyama A, Harada K, shimada S, Saida T, Ferrone S, Takata M, Uhara H, Okuyama R
    • Journal Title

      British J Cancer

      Volume: 106 Pages: 939-946

    • Peer Reviewed
  • [Presentation] Assessment of NRAS mutation in Japanese melanoma patients2013

    • Author(s)
      Ashida A, Uhara H, Okuyama R
    • Organizer
      The 72th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      Yokohama, JPN
    • Year and Date
      20131003-05
  • [Presentation] 日本人のメラノーマ患者におけるBRAF、NRAS、KITの遺伝子変異の解析2013

    • Author(s)
      芦田敦子、宇原久、奥山隆平
    • Organizer
      日本皮膚悪性腫瘍学会
    • Place of Presentation
      甲府
    • Year and Date
      20130809-10
  • [Presentation] Assessment of BRAF and KIT mutation in Japanese melanoma patients2012

    • Author(s)
      Ashida A, Uhara H, Murata H, Goto Y, Okuyama R
    • Organizer
      The 71th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      Sapporo, JPN
    • Year and Date
      20120919-21
  • [Book] 皮膚の悪性腫瘍II悪性黒色腫:分子標的薬KITの変異に基づく治療薬,皮膚科臨床アセット172014

    • Author(s)
      芦田敦子、奥山隆平
    • Total Pages
      59-64

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Published: 2015-07-16  

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