Analysis of blistering mechanism in pemphigus.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591620
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Okayama University
• Principal Investigator: YUMI AOYAMA 岡山大学, 医歯(薬)学総合研究科, 准教授 (90291393)
• Project Period (FY): 2011 – 2013
• Keywords: 天疱瘡 / 自己免疫 / ケラチノサイト

Research Abstract

Pemphigus is an intractable autoimmune blistering disease caused by IgG autoantibodies against the extracellular domains (ECs) of desmogleins (Dsgs). We have modified enzyme-linked immunosorbent assay (ELISA) using ethylenediaminetetraacetic acid (EDTA) to detect only nonpathogenic anti-Dsg3 antibodies against the non-calcium (Ca2+)-dependent epitopes. Using this method, anti-Dsg3 serum antibodies against the Ca2+-dependent epitopes can be obtained. In this study, we examined the reliability of the ELISA-determined activities of anti-Dsg3 serum antibodies against the Ca2+-dependent epitopes in clinical cases and demonstrated a significant correlation between the ELISA-determined activities of anti-Dsg3 serum antibodies against the Ca2+-dependent conformational epitopes and the clinical disease activities of PV patients. The conformational Dsg3 ELISA index showed improved correlation to the disease activity when monitored in individual cases.

Research Products

• [Journal Article] Detection of Autoantibodies to Precursor Proteins of Desmogleins in Sera of a Patient with Bowen Carcinoma (2014)
  • Author(s): Demitsu T, Yamada T, Nakamura S, Kakurai M, Dohmoto T, Kamiya K, Aoyama Y, Iwatsuki K, Yamagami J, Ohyama B, Ohata C, Koga H, Hashimoto T
  • Journal Title: Acta Derm Venereol
  • DOI: 10.2340/00015555-1776
  • Peer Reviewed
• [Journal Article] Concurrence of autoantibodies to bullous pemphigoid antigens and desmoglein 3: analysis of pathogenic and nonpathogenic antibodies (2013)
  • Author(s): Ono S, Tanioka M, Tanizaki H, Fujisawa A, Koga H, Hashimoto T, Kamiya K, Aoyama Y, Iwatsuki K, Miyachi Y
  • Journal Title: Br J Dermatol Volume: 168(6) Pages: 1357-60
  • DOI: 10.1111/bjd.12143
  • Peer Reviewed
• [Journal Article] A higher correlation of the antibody activities against the calcium-dependent epitopes of desmoglein 3 quantified by ethylenediaminetetraacetic acid-treated enzyme-linked immunosorbent assay with clinical disease activities of pemphigus vulgaris (2013)
  • Author(s): Kamiya K, Aoyama Y, Shirafuji Y, Hamada T, Morizane S, Fujii K, Iwatsuki K
  • Journal Title: J Dermatol Sci Volume: 70(3) Pages: 190-5
  • DOI: 10.1016/j.jdermsci
  • Peer Reviewed
• [Journal Article] Case of subepidermal blistering disease with autoantibodies to both laminin-γ1 and laminin-α3 (2013)
  • Author(s): Kamiya K, Aoyama Y, Kawase K, Schmidt E, Zillikens D, Kitajima Y, Iwatsuki K
  • Journal Title: J Dermatol Volume: 40(6) Pages: 491-2
  • DOI: 10.1111/1346-8138.12127
  • Peer Reviewed
• [Journal Article] Detection of antibodies against the non-calcium-dependent epitopes of desmoglein 3 in pemphigus vulgaris and their pathogenic significance (2012)
  • Author(s): Kamiya K, Aoyama Y, Shirafuji Y, Hamada T, Morizane S, Fujii K, Hisata K, Iwatsuki K
  • Journal Title: Br J Dermatol Volume: 167(2) Pages: 252-61
  • DOI: 10.1111/j.1365-2133.2012.10929.x
  • Peer Reviewed
• [Presentation] The epitopes and IgG subclasses of anti-desmoglein antibodies detected in individuals without pemphigus (2011)
  • Author(s): Koji Kamiya, Yumi Aoyama, Yoshinori Shirafuji, Toshihisa Hamada, Shin Morizane, Kazuyasu Fujii, Keiji Iwatsuki
  • Organizer: 第36回日本研究皮膚科学会学術大会・総会
  • Place of Presentation: 京都
  • Year and Date: 2011-12-11