2013 Fiscal Year Final Research Report
detailed characterization of autoantibodies in paraneoplastic pemphigus by phage display
| Project/Area Number |
23591628
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Toho University (2012-2013)
Teikyo University (2011) |
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Principal Investigator |
ISHII Ken 東邦大学, 医学部, 准教授 (50296670)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 皮膚科学 / 自己免疫性疾患 / 細胞接着 |
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| Research Abstract |
To study the pathogenic mechanism of pareneoplastic pemphigus(PNP), we used phage display to clone monoclonal anti-Dsg3 antibodies from a PNP patient. We isolated 20 unique Dsg3-reactive mAbs, which we classified into four groups according to amino acid sequence. Pathogenic assay showed that three antibodies displayed pathogenic activity in blister formation with different potencies. Epitope mapping showed that these pathogenic mAbs bound Ca(2+)-dependent conformational epitopes in the middle portion of the extracellular region of Dsg3. These mAbs reflect the unique polyclonal nature of anti-Dsg3 antibodies in PNP and represent an important tool for detailing the pathophysiological mechanisms of blister formation in PNP.
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Research Products
(10 results)
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[Journal Article] Development of NC1 and NC2 domains of Type VII collagen ELISA for the diagnosis and analysis of the time course of epidermolysis bullosa acquisita patients2011
Author(s)
Saleh MA, Ishii K, Kim YJ, Murakami A, Ishii N, Hashimoto T, Schmidt E, Zillikens D, Shirakata Y, Hashimoto K, Kitajima Y, Amagai M
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Journal Title
J Dermatol Sci
Volume: 62(3)
Pages: 169-175
DOI
Peer Reviewed
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