2013 Fiscal Year Final Research Report
Early differential diagnosis of malignant melanoma by abnormal DNA methylation
| Project/Area Number |
23591630
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Nihon University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TERUI Tadashi 日本大学, 医学部, 教授 (30172109)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 皮膚科学 / 皮膚腫瘍学 / 癌 / 遺伝学 / ゲノム |
|---|
| Research Abstract |
In order to identify the early tumor marker of malignant melanoma, Sequenom MassARRAY quantitative methylation analysis using the MassARRAY Compact System was performed for the quantitative DNA methylation analysis of ZAR1 gene. Fifth surgical specimens (e.g. melanocytic nevus, Spitz nevus, dysplastic nevus, and melanoma in situ) that were similar to malignant melanoma by pathologic diagnosis were examined. DNA were collected by using Leica Laser Microdissection systems. However, the extraction of DNA and the quantitative DNA methylation analysis of ZAR1 gene using the MassARRAY were difficult, therefore we were not able to analyze.
|
