2013 Fiscal Year Final Research Report
Development of mouse model for autoinflammatory disease, TRAPS (TNF-receptor associated periodic syndrome)
| Project/Area Number |
23591662
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 自己炎症性疾患 / ＴＲＡＰＳ / ＴＮＦ受容体 / マウス |
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| Research Abstract |
To address the pathomechanism of autoinflammatory disease, TRAPS (TNF receptor associated periodic syndrome), we tried to develop a mouse model of TRAPS by a forced expression of mutant TNF receptor. cDNA with a mutation in TNF receptor gene was ligated into the expression plasmid vector under the CMV promoter. This plasmid was linearized and cut with restriction enzymes and injected into C57BL/6J mice fertilized eggs. Out of 99 mouse specimens analyzed with PCR (polymerase chain reaction), 3 specimens were found to contain inserted plasmid DNA. One mouse died in utero, one mouse died within a few weeks after the birth and one mouse passed away at the age of 6 weeks after birth. The transgenic mouse at 6 weeks after the birth showed retardation in growth and abnormalities in hair follicle development and/or hair cycling. Taken together, these results suggested an important role of normal TNF receptor for development of hair follicles and other unspecified organs.
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Research Products
(9 results)
