Interstitial pathophysiology and angiogenesis and its imaging in myocardial ischemia and remodeling

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591756
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Kanazawa University
• Principal Investigator: TAKI Junichi 金沢大学, 大学病院, 講師 (10251927)
• Co-Investigator(Kenkyū-buntansha): SHIBA Kazuhiro 金沢大学, 学際科学実験センター, 教授 (40143929) ; OGAWA Kazuma 金沢大学, 薬学系, 准教授 (30347471)
• Project Period (FY): 2011 – 2013
• Keywords: 心筋虚血 / 心筋梗塞 / メチオニン / 血管新生 / リモデリング / マクロファージ / 心筋繊維芽細胞 / テネイシンC

Research Abstract

We investigated the serial changes and mechanisms of C-14-methionine uptake in a rat model of myocardial ischemia and reperfusion. Both C-14-methionine and Tl-201 uptake were reduced in the area at risk at 1 day after reperfusion. However, 3 days after reperfusion, an increased C-14-methionine uptake was observed corresponding to the area of still reduced Tl-201 uptake, and the C-14-Methionine uptake gradually declined until 28 days. The C-14-methionine uptake area at 3-7 days corresponded well to the macrophage infiltrations demonstrated by positive CD68 staining. Anti alpha-SMA staining appeared at 7 days, after which CD68 staining was gradually replaced by the SMA staining, suggesting that methionine uptake in the early phase after ischemia and reperfusion might reflect inflammatory activity. Methionine imaging may be useful for inflammatory imaging early after myocardial infarction.

Research Products

• [Journal Article] ^<14>C-Methionine Uptake as a Potential Marker of Inflammatory Process after Myocardial Ischemia and Reperfusion (2013)
  • Author(s): Taki, J., Wakabayashi, H.,Inaki, A., Imanaka-Yoshida, K., Hiroe, M., Ogawa, K., Morooka M., Kubota K., Shiba, K., Yoshida, T., Kinuya, S.
  • Journal Title: J Nucl Med Volume: 54 Pages: 431–436
  • Peer Reviewed
• [Journal Article] Editorial : Potential utility of 99mTc-annexin V imaging in ischemically damaged myocardium (2011)
  • Author(s): Taki J., Matsunari I., Wakabayashi H., Inaki A., Kinuya S.
  • Journal Title: Intv Cardiol Volume: 2 Pages: 765-767
  • Peer Reviewed
• [Presentation] C-14-Methionine Accumulation as a Potential Marker of Inflammatory Process after Myocardial Ischemia and Reperfusion (2013)
  • Author(s): Taki, J., Wakabayashi, H., Imanaka -Yoshida, K., Matsunari, I., Hiroe, M.
  • Organizer: The 77th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Yokohama
  • Year and Date: 2013-03-15
• [Presentation] ^1 Postconditioning attenuates interstitial tissue remodeling in a rat model of severe ischemia and reperfusion : Assessment by I-125-anti tenascin-C antibody imaging (2012)
  • Author(s): Taki, J., Wakabayashi, H.,Inaki, A., Imanaka-Yoshida, K., Hiroe, M., Ogawa, K., Shiba, K., Kinuya, S.
  • Organizer: 59th Annual Meeting of the Society of Nuclear Medicine
  • Place of Presentation: Miami Beach Convention Center (USA)
  • Year and Date: 2012-06-12
• [Presentation] Spatial and temporal change of C-14-methionine uptake in a rat model of acute ischemia and reperfusion (2011)
  • Author(s): Taki, J., Wakabayashi, H.,Inaki, A., Ogawa, K., Shiba, K., Morooka, M., Kubota, K., Hiroe, M., Kinuya, S.
  • Organizer: 58th Annual Meeting of the Society of Nuclear Medicine
  • Place of Presentation: San Antonio Convention Center (USA)
  • Year and Date: 2011-06-06
• [Book] Role of Fatty Acid Imaging with ^<123>I-β-p-^<123>I-Iodophenyle -Pentadecanoic Acid (^<123>I-BMIPP) in Ischemic Heart Diseases. Ischemic Heart Disease (2013)
  • Author(s): Taki, J., Matsunari, I., Wakabayashi, H., Inaki, A., Kinuya, S.
  • Total Pages: 175-200
  • Publisher: INTECH
• [Book] Apoptosis Imaging in Diseased Myocardium. 12 Chapters in Nuclear Medicne (2011)
  • Author(s): Taki, J., Wakabayashi, H., Inaki, A., Matsunari, I., Kinuya, S.
  • Total Pages: 251-264
  • Publisher: INTECH