Mechanism elucidation of proton beam specific cellular response for radiation therapy

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591854
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: The Wakasa Wan Energy Research Center
• Principal Investigator: HATASHITA masanori 公益財団法人若狭湾エネルギー研究センター, 研究開発部, 主任研究員 (40359237)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Hideki 福井大学, 高エネルギー医学研究センター, 准教授 (40142377)
• Project Period (FY): 2011 – 2013
• Keywords: 量子ビーム / イオンビーム / 突然変異 / 放射線感受性

Research Abstract

The differences in mutation induction and mutation spectrum between proton beams and X-rays in human cells were investigated. Cell killing effect was almost the same between proton beams and X-rays, while a difference in mutation frequency was observed and the frequency of hprt mutation of cells irradiated with proton beams was lower than that of cells irradiated with X-rays. Furthermore, the deletion spectrum of exons was different in induced mutants between proton beams and X-rays. These findings suggest that there are quantitative and qualitative differences in mutation induction between proton beams and X-rays even if the RBE values are similar.

Research Products

• [Journal Article] Sinodielide A exerts thermosensitizing effects and induces apoptosis and G_2/M cell cycle arrest in DU145 human prostate cancer cells via the Ras/Raf/MAPK and PI3K/Akt signaling pathways (2014)
  • Author(s): M. Hatashita, M. Taniguchi, K. Baba, K. Koshiba, T. Sato, Y. Jujo, R. Suzuki, S. Hayashi
  • Journal Title: International Journal of Molecular Medicine Volume: 33(2) Pages: 406-414
  • DOI: 10.3892/ijmm.2013.1568
  • Peer Reviewed
• [Journal Article] 放射線応答性遺伝子発現制御システムの開発 (2013)
  • Author(s): 小川良平、森井章裕、渡部明彦、崔正国、鍵谷豪、畑下昌範、久米恭、福田茂一、長谷川崇、近藤隆
  • Journal Title: 放射線生物研究 Volume: 48(3) Pages: 267-279
  • URL: http://rbrc.kenkyuukai.jp/special/index.asp?id=11654
  • Peer Reviewed
• [Journal Article] Regulation of gene expression in retrovirus vectors by X-ray and proton beam radiation with artificially constructed promoters (2012)
  • Author(s): R. Ogawa, A. Morii, A. Watanabe, Z.-G. Cui, Kagiya, S. Fukuda, K. Kume, T. Hasegawa, M. Hatashita, H. Izumi, T. Ishimoto, L. B. Feril Jr
  • Journal Title: The Journal of Gene Medicine. Volume: 14(5) Pages: 316-327
  • DOI: 10.1002/jgm.2625
  • Peer Reviewed
• [Journal Article] Nitric Oxide is a key molecule serving as a bridge between radiation-induced bystander and adaptive responses (2011)
  • Author(s): Matsumoto, M. Tomita, K. Otsuka, M. Hatashita, N. Hamada
  • Journal Title: Curr Mol Pharmacol Volume: 4(2) Pages: 126-134
  • DOI: 10.2174/1874467211104020126
  • Peer Reviewed
• [Presentation] Response of Human Cells to Proton Irradiation Compared to X-Ray Irradiation (2011)
  • Author(s): M. Hatashita, K. Takagi, K. Kume, G. Kagiya and H. Matsumoto
  • Organizer: The 14th International Congress of Radiation Research
  • Place of Presentation: Poland
  • Year and Date: 2011-08-30
• [Presentation] Nitric Oxide is a Key Molecule Serving as a Bridge between Radiation-Induced Bystander and Adaptive Responses. (2011)
  • Author(s): H. Matsumoto, M. Tomita, K. Otsuka, M. Maeda, N. Hamada, M. Hatashita
  • Organizer: The 14th International Congress of Radiation Research
  • Place of Presentation: Poland
  • Year and Date: 2011-08-30