Regenerative pancreatic islet transplantation for the young onset Type I diabetes mellitus. It is xenotransplantation of the pancreatic islet cell sheet.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591871
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Fukushima Medical University
• Principal Investigator: ISHII Show 福島県立医科大学, 医学部, 助教 (40468129)
• Co-Investigator(Kenkyū-buntansha): ISE Kazuya 公立大学法人福島県立医科大学, 医学部, 講師 (90363746) ; SHIIZU Hirofumi 公立大学法人福島県立医科大学, 医学部, 助教 (70553709)
• Co-Investigator(Renkei-kenkyūsha): GOTOH Mitsukazu 公立大学法人福島県立医科大学, 医学部, 教授 (50162160)
• Project Period (FY): 2011 – 2013
• Keywords: 細胞シート / I型糖尿病 / 膵島移植 / 膵島再生 / ティッシュエンジニアリング / 異種移植

Research Abstract

The present study was conducted to develop a dispersed islet cell-based tissue engineering approach for the treatment of diabetes mellitus. Dispersed islet cells obtained from rats were then plated on the laminin-5-PIPAAm dishes. At day 2 of culture, cultured islet cells were harvested as a uniformly connected tissue sheet by lowering the culture temperature from 37 degrees C to 20 degrees C for 30 min. Two harvested islet cell sheets were transplanted for engineering neo-islet tissues in the subcutaneous site of streptozotocin-induced diabetic mice. The diabetic mice that received islet sheet transplantation did not revert from hyperglycemia. Histological examination revealed that the transplanted islet cell sheets formed clusters of islet tissues and inflammatory cell infiltration. The present study may describe a new proof-of-concept therapeutic approach to engineer functional neo-islet tissues for xenotransplantation.

Research Products

• [Journal Article] Topographical arrangement ofα- andβ- cells tissues engineered by islet cell sheet transplantation in mice (2013)
  • Author(s): Shimizu H, Ohashi K, Saito T, Utoh R, Ise K, Suzuki H, Yamato M, Okano T, Gotoh M
  • Journal Title: Transplantation Proceedings Volume: 45 Pages: 1881-4
  • DOI: 10.1016/j.transproceed.2013.01.003
• [Journal Article] Preservation of pancreatic islets in cold UW solution before transplantation (2012)
  • Author(s): Ishii S, Saito T, Ise K, Yamashita M, Saito T, Tsukada M, Oshibe I, Kenjo A, Kimura T, Anazawa T, Suzuki H, Gotoh M
  • Journal Title: Islets Volume: 4 Pages: 32-9
  • DOI: 10.4161/isl.18607
• [Journal Article] Reversal of Diabetes by the Creation of Neo-islet Tissues into a Subcutaneous Site Using Islet Cell Sheets (2011)
  • Author(s): Saito T, Ohashi K, Utoh R, Shimizu H, Ise K, Suzuki H, Yamato M, Okano T, Gotoh M
  • Journal Title: Transplantation Volume: 29(11) Pages: 1231-6
• [Presentation] Long-term outcome of clinical islet transplantation using donors after cardiac death : a multicenter experience in Japan (2013)
  • Author(s): Anazawa T, Kenjo A, Kimura T, Ise K, Haga J, Sato N, Tsuchiya T, Sato T, Gotoh M
  • Organizer: The 14th World congress of the international pancreas and islet transplantation association
  • Place of Presentation: Monterey, CA, US
  • Year and Date: 20130924-27
• [Presentation] Reassembly of alpha and beta cells of neo-islet tissues engineered in the subcutaneas space by islet-cell sheet transplantation in mice (2013)
  • Author(s): Shimizu H, Utoh R, Hanayama H, Ise K, Yamato M, Okano T, Gotoh M
  • Organizer: The 14th World congress of the international pancreas and islet transplantation association
  • Place of Presentation: Monterey, CA, US
  • Year and Date: 20130924-27
• [Presentation] Adenoviral vector-megiated anti-apoptptic gene transduction for engineering functional islet cell sheets (2013)
  • Author(s): Hanayama H, Ohashi K, Utoh R, Ise K, Shimizu T, Yamato M, Mizuguchi H, Sakurai F, Okano T, Gotoh M
  • Organizer: The 14th World congress of the international pancreas and islet transplantation association
  • Place of Presentation: Monterey, CA, US
  • Year and Date: 20130924-27
• [Presentation] Long-term follow-up of clinical islet transplantation using donors after cardiac death report of Japan islet transplantation registry (2013)
  • Author(s): Anazawa T, Kenjo A, Kimura T, Ise K, Haga J, Sato N, Tsuchiya T, Sato T, Gotoh M
  • Organizer: The 13th congress of the Asian society of transplantation
  • Place of Presentation: 京都
  • Year and Date: 20130902-06
• [Presentation] 再生医学研究が変える膵島移植医療の近未来 (2013)
  • Author(s): 穴澤貴行、土屋貴男、見城明、伊勢一哉、木村隆、芳賀淳一郎、佐藤直哉、後藤満一
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡
  • Year and Date: 20130411-13
• [Presentation] 膵島移植の新たな展開 (2011)
  • Author(s): 清水裕史、大橋一夫、斎藤敬弘、鵜頭理恵、伊勢一哉、大和雅之、岡野光夫、後藤満一
  • Organizer: 第36回日本外科系連合会学術集会
  • Place of Presentation: 幕張
  • Year and Date: 2011-06-16
• [Presentation] Functional islet tissue engineering is subcutaneous site using fabricated islet cell sheets (2011)
  • Author(s): Shimizu H, Ohashi K, Saito T, Utoh R, Ise K, Yamato M, Okano T, Gotoh M
  • Organizer: The 13th World congress of international pancreas and islet transplantation association
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2011-06-03
• [Presentation] Mitomycin-C treatment protects islets from the progression of central damage during culture (2011)
  • Author(s): Haga J, Saito T, Anazawa T, Tsuchiya T, Kenjo A, Sato S, Saito T, Ise K, Yamamoto M, Waguri S, Gotoh M
  • Organizer: The 13th World congress of international pancreas and islet transplantation association
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2011-06-03