Study of the mechanism of IL-10 to induce microchimerism in organ transplant model.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591873
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: YOSHIMURA NORIO 京都府立医科大学, 医学(系)研究科(研究院), 教授 (00191643)
• Co-Investigator(Kenkyū-buntansha): MATSUYAMA Masahide 京都府立医科大学, 医学研究科, 講師 (80549767)
• Project Period (FY): 2011 – 2013
• Keywords: マイクロキメリズム / IL-10 / マウス異所性心移植モデル / 免疫寛容

Research Abstract

(AIMS)We investigated that transduction of the human Interleukin-10 (IL-10) gene in mouse foetal liver stem cells (hIL-10-TFLs) protects them from rejection in an allogeneic host and the effects of these cells in organ transplantation.(METHODS)Balb/c mice were sublethally irradiated before receiving vascularized heterotopic heart grafts from C57BL/6 mice. TFLs from C57BL/6 mice transduced with hIL-10, were injected on the day of transplantation, every 20 days.(RESULTS)Heart transplants survived 86.25 versus 46.3 or 11.5 days, in mice which received repeated injections, a single injection, or in control mice. Expressions of H-2b and hIL-10 were found in thymus, liver. Foxp3+ cells present inside the transplanted heart.(CONCLUSIONS)We have shown that repeated injections of hIL-10-TFLs are efficient in impairing transplant rejection. This"prope tolerance"was associated with survival of donor haematopoietic cells in the host.

Research Products

• [Journal Article] Prope tolerance to heart allografts in mice associated with persistence of donor interleukin-10-transduced stem cells (2012)
  • Author(s): Brikci-Nigassa L, Matsuyama M, Hase T, Eljaafari A, Chargui J, Sanhadji K, Inori F, Nakatani T, Yoshimura R, Touraine JL
  • Journal Title: T ran sp lan tation 27 Volume: 93(8) Pages: 761-8
• [Presentation] Prope Tolerance"The possibility of donor Interleukin-10-transducted foetal liver stem cells in mice heart allograft (2012)
  • Author(s): Matsuyama M, Hase T, Yoshimura R, Brikci-Nigassa L, Chargui J, Koshino K, Sakai K, Suzuki T, Nobori S, Ushogome H, Okajima H, Touraine JL, Yoshimura N
  • Organizer: 49th European Renal Association-European Dialysis and Transplantation Association Congress
  • Place of Presentation: Paris, France(国際学会Award (賞))
  • Year and Date: 2012-05-26
• [Presentation] 国内学会シンポジウムマウス異所性心移植モデルを用いたIL-10での免疫寛容 (2011)
  • Author(s): 松山昌秀、長谷太郎、吉村力勇、仲谷達也、吉村了勇
  • Organizer: 第47回日本移植学会総会
  • Place of Presentation: 仙台(国内学会シンポジウム)
  • Year and Date: 2011-10-06