2013 Fiscal Year Final Research Report
The role of the adipocyte function after the surgical stress: the effectiveness of the pioglitazone administration
| Project/Area Number |
23591880
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 脂肪細胞 / 手術侵襲 / アディポサイトカイン / ピオグリタゾン |
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| Research Abstract |
To investigate the effect of PPAR-gamma (PPARg) agonist on cecal ligation and puncture (CLP)-induced adipose-tissue inflammation and lung injury in mice. Mice were assigned to 3 groups: 1) CLP group, 2) PPARg agonist-treated CLP group, or 3) sham group. PPARg agonist was injected intraperitonally for 7 days. TNF-alpha (TNF) levels in serum and peritoneal lavage fluid were significantly higher in the CLP group than in the PPARg agonist-treated CLP group. PPARg agonist treatment decreased the TNF mRNA expression levels as compared with the CLP group. Histopathological analysis of lung tissue revealed significantly increased numbers of TUNEL-positive cells in the CLP group as compared with the sham-operated control group. PPARg agonist treatment reduced the CLP-induced cell and tissue injury as compared with the CLP group. PPARg agonist effectively suggested to prevent lung injury caused by CLP-induced sepsis by maintaining the anti-inflammatory status of adipose tissue.
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