2013 Fiscal Year Final Research Report
Basic and clinical research of circulating DNA fragments as a biomarker in gastroenterological cancer.
| Project/Area Number |
23591907
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Tokai University |
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Principal Investigator |
OZAWA Soji 東海大学, 医学部, 教授 (10169287)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYACHI Hayato 東海大学, 医学部, 教授 (20174196)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 癌 / 核酸 / 外科 / 遺伝子 |
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| Research Abstract |
Human squamous cancer cells (TE-4) were inoculated subcutaneously in the bilateral back of BALB/c nude mice. Unilateral tumors or bilateral tumors were resected 3 weeks after inoculation as surgical models. And 5-FU (25mg/kg or 50mg/kg) was injected intraperitoneously 4 times 3 weeks after inoculation as chemotherapy models. Blood was collected and animals were sacrificed 5 weeks after inoculation. Serum was processed and used for DNA measurement using a real time PCR method. DNA concentrations (Alu 247 and Alu115) correlated with tumor volumes. Serum DNA seems to be a good biomarker for cancer staging and evaluating chemotherapeutic effects.
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