2013 Fiscal Year Final Research Report
Pancreatic cancer model with in vivo imaging and therapeutic trial
| Project/Area Number |
23591908
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
UCHIDA EIJI 日本医科大学, 医学(系)研究科(研究院), 教授 (70176684)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 膵癌 / 実験モデル / イメージング / 治療実験 |
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| Research Abstract |
We established several pancreatic cancer models using hamster pancreatic cancer cell line, PGHAM-1. PGHAM-1 cells with luciferase activity transduced by viral vectors (PGHAM-1-Luc) ware used for in vivo imaging of cancer proliferation and metastasis using IVIS system by measuring the bioluminescent signals. Using this system, the effect of gemcitabine on pancreatic cancer model was investigated in vitro and in vivo. Anti-tumor effect of gemcitabine was found in vitro but not in vivo by using IVIS system quantitative analysis. This cancer model is suitable for detecting the new therapeutic agents, although improved in vivo imaging system and its application are needed in the near future.
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