2013 Fiscal Year Final Research Report
Quantitative detection of circulating tumor DNA in the plasma of gastric cancer patients
| Project/Area Number |
23591928
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
KUROKAWA Yukinori 大阪大学, 医学(系)研究科(研究院), 助教 (10470197)
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| Co-Investigator(Kenkyū-buntansha) |
TAKIGUCHI Shuji 大阪大学, 医学系研究科, 講師 (00301268)
KATO Kikuya 大阪府立成人病センター研究所, 所長 (60194809)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 胃癌 / ctDNA / BEAMing / 次世代シーケンサー |
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| Research Abstract |
We performed quantitative detection of circulating tumor DNA (ctDNA) that harbors gene mutations identical to the primary tumor, by analyzing cell-free DNA in the plasma of gastric cancer patients using BEAMing or next generation sequencer. 3 out of 6 advanced gastric cancer patients showed positive ctDNA for TP53 before surgery. ctDNA level corresponded to surgical resection, tumor recurrence, and response to chemotherapy. We demonstrated that ctDNA might be useful biomarker for monitoring tumor progression in gastric cancer patients.
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