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2013 Fiscal Year Final Research Report

Pseudogene affected gene expression via regulating microRNA in esopheal squamous cancer

Research Project

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Project/Area Number 23591940
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionKumamoto University

Principal Investigator

NAGAI Yohei  熊本大学, 医学部附属病院, 非常勤診療医師 (30551254)

Co-Investigator(Kenkyū-buntansha) WATANABE Masayuki  公益財団法人がん研究会, がん研有明病院, 食道担当部長 (80254639)
HAYASHI Naoko  熊本大学, 医学部附属病院, 非常勤診療医師 (20452899)
BABA Yoshifumi  熊本大学, 大学院生命科学研究部, 講師 (20599708)
Project Period (FY) 2011 – 2013
KeywordsmicroRNA / psuedogene / FBXW7
Research Abstract

MicroRNAs (miRsare) ~22 nucleotide non-coding RNA molecules that regulate gene expression post-transcriptionally. Competitive endogenous RNAs (ceRNAs) sequester miRs to regulate mRNA transcripts containing common microRNA recognition elements. We investigated the relationship among the PTEN, PTENP1 by qRT-PCR in 40 cancer cell lines. In the 40 gastric cancer patients, we found a remarkably strong correlation between the expression revels of the PTEN and PTENP1. Next, we indicates that high expression of miR-223 had a significant adverse impact on the survival of esophageal squamous cell carcinoma patients through repression of the function of FBXW7. We sought that 3'UTR of FBXW7 will be therapy sponge which decoy of onco-miRs targeted FBXW7. However, up-regulated 3'UTR of FBXW7 did not affect the expression of miRs and FBXW7. Next, we suggests that miR-200b regulates the ZEB1/2 in gastric cancer and thus controls EMT in gastric cancer.

  • Research Products

    (12 results)

All 2013 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (6 results) Book (1 results)

  • [Journal Article] Clinical impact of serum exosomal microRNA-21 as a clinical biomarker in human esophageal squamous cell carcinoma2013

    • Author(s)
      Tanaka Y, Kamohara H, Kinoshita K, Kurashige J, Ishimoto T, Iwatsuki M, Watanabe M, Baba H.
    • Journal Title

      Cancer

      Volume: 119(6) Pages: 1159-67

    • DOI

      10.1002/cncr.27895

    • Peer Reviewed
  • [Journal Article] Overexpression of microRNA-223 regulates the ubiquitin ligase FBXW7 in oesophageal squamous cell carcinoma2012

    • Author(s)
      Kurashige J, Watanabe M, Iwatsuki M, Kinoshita K, Saito S, Hiyoshi Y, Kamohara H, Baba Y, Mimori K, Baba H.
    • Journal Title

      Br J Cancer

      Volume: 106(1) Pages: 182-8

    • DOI

      10.1038/bjc.2011.509

    • Peer Reviewed
  • [Journal Article] MicroRNA-200b Regulates Cell Proliferation, Invasion, and Migration by Directly Targeting ZEB2 in Gastric Carcinoma2012

    • Author(s)
      Kurashige J, Kamohara H, Watanabe M, Hiyoshi Y, Iwatsuki M, Tanaka Y, Kinoshita K, Saito S, Baba Y, Baba H.
    • Journal Title

      Ann Surg Oncol

      Volume: 19 Suppl 3 Pages: 656-64

    • DOI

      10.1245/s10434-012-2217-6

    • Peer Reviewed
  • [Journal Article] Serum microRNA-21 is a novel biomarker in patients with esophageal squamous cell carcinoma2012

    • Author(s)
      Kurashige J, Kamohara H, Watanabe M, Tanaka Y, Kinoshita K, Saito S, Hiyoshi Y, Iwatsuki M, Baba Y, Baba H.
    • Journal Title

      J Surg Oncol

      Volume: 106 Pages: 188-92

    • DOI

      10.1002/jso.23064

    • Peer Reviewed
  • [Journal Article] Clinical significance of Wnt-induced secreted protein-1 (WISP-1/CCN4) in esophageal squamous cell carcinoma2011

    • Author(s)
      Nagai Y, Watanabe M, Ishikawa S, Karashima R, Kurashige J, Iwagami S, Iwatsuki M, Baba Y, Imamura Y, Hayashi N, Baba H.
    • Journal Title

      Anticancer Res.

      Volume: 31(3) Pages: 991-7

    • URL

      http://ar.iiarjournals.org/content/31/3/991.full.pdf+html

    • Peer Reviewed
  • [Presentation] miR-223は食道扁平上皮癌において癌抑制遺伝子ユビキチンリガーゼ複合体基質ユニットFBXW7の発現を制御する2012

    • Author(s)
      藏重淳二、渡邊雅之、岩槻政晃、馬場秀夫、森正樹、三森功士
    • Organizer
      第21回日本がん転移学会学術集会
    • Place of Presentation
      オリエンタルホテル広島
    • Year and Date
      20120712-13
  • [Presentation] 食道扁平上皮癌においてmicroRNA-223は癌抑制遺伝子ユビキチンリガーゼ複合体基質ユニットFBXW7の発現を制御する2012

    • Author(s)
      藏重淳二、渡邊雅之、田中洋平、木下浩一、齋藤誠哉、岩槻政晃、馬場祥史、馬場秀夫
    • Organizer
      第112回日本外科学会定期学術集会
    • Place of Presentation
      幕張メッセ(千葉県)
    • Year and Date
      20120412-14
  • [Presentation] Overexpression of microRNA-223 regulates the ubiquitin ligase FBXW7 in esophageal squamous cell carcinoma2012

    • Author(s)
      Kurashige J, Watanabe M, Iwatsuki M, Baba Y, Baba H
    • Organizer
      AACR Annual Meeting 2012
    • Place of Presentation
      アメリカ合衆国シカゴ
    • Year and Date
      20120401-04
  • [Presentation] 胃癌におけるmicroRNA-200familyの発現と臨床病理学的検討2012

    • Author(s)
      藏重淳二、蒲原英伸、渡邊雅之、田中洋平、木下浩一、齋藤誠也、岩槻政晃、馬場祥史、馬場秀夫
    • Organizer
      第84回日本胃癌学会総会
    • Place of Presentation
      大阪国際会議場(グランキューブ大阪)
    • Year and Date
      20120208-10
  • [Presentation] MicroRNA-200b regulates Epithelial Mesenchymal Transition by targeting ZEB2 in Gastric Cancer2011

    • Author(s)
      Junji Kurashige , Masayuki Watanabe, Yukiharu Hiyoshi , Hidenobu Kamohara , Koichi Kinoshita, Seiya Saito, Masaaki Iwatsuki, Yoshifumi Baba , Naoko Hayashi , Hideo Baba
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Year and Date
      20111003-05
  • [Presentation] 胃癌におけるmicroRNA-200bによる浸潤転移機構の制御2011

    • Author(s)
      藏重淳二、渡邊雅之、田中洋平、木下浩一、齋藤誠也、日吉幸晴、岩槻政晃、馬場祥史、馬場秀夫
    • Organizer
      第20回日本がん転移学会学術集会
    • Place of Presentation
      アクトシティ浜松(静岡県)
    • Year and Date
      20110712-13
  • [Book] 最新胃癌学基礎と臨床の最新研究動向「胃癌におけるマイクロRNA-200bの意義」2013

    • Author(s)
      藏重淳二、渡邊雅之、岩槻政晃、岩上志朗、馬場秀夫
    • Total Pages
      159-165

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Published: 2015-07-16  

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