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2013 Fiscal Year Final Research Report

Global analysis of genomic alterations associated with AKT and ERK pathway in esophageal squamous cell carcinoma

Research Project

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Project/Area Number 23591941
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionKumamoto University

Principal Investigator

MIYANARI Nobutomo  熊本大学, 医学部附属病院, 非常勤診療医師 (90336230)

Co-Investigator(Kenkyū-buntansha) BABA Hideo  熊本大学, 大学院生命科学研究部, 教授 (20240905)
BABA Yoshifumi  熊本大学, 大学院生命科学研究部, 講師 (20599708)
IWATSUKI Masaaki  熊本大学, 医学部附属病院, 非常勤診療医師 (50452777)
Project Period (FY) 2011 – 2013
Keywordsgenomic alteration / 食道扁平上皮癌 / KRAS / BRAF / PIK3CA
Research Abstract

Using resected ESCC specimens,we investigate mutations of KRAS,BRAF and PIK3CA by Pyrosequencing technology.As a result,the KRAS mutation was found to be extremely rare(1 of 203;0.5%),and the BRAF mutation was absent(0 of 203;0%),suggesting that KRAS and BRAF mutations play a limited role in the development of ESCC.However,PIK3CA mutations mutations in ESCC are associated with longer overall survival and cancer-specific survival,suggesting its role as a prognostic biomarker.We evaluated colorectal cancer with synchronous liver metastases. We evaluated KRAS,BRAF,and PIK3CA mutation status and microsatellite instability(MSI) status in 42 liver metastases, 26 matched primary tumors, and 6 matched lymph node(LN) metastases by Pyrosequencing technology.We also quantified LINE-1 methylation levels.The concordance rate was 94% for KRAS,100% for BRAF,88% for PIK3CA,and 97% for MSI.Primary tumors showed LINE-1 methylation levels similar to those of matched liver and LN metastases.

  • Research Products

    (9 results)

All 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (6 results)

  • [Journal Article] PIK3CA mutation is associated with a favorable prognosis among patients with curatively resected esophageal squamous cell carcinoma2013

    • Author(s)
      Shigaki H, Baba Y, Watanabe M, Murata A, Ishimoto T, Iwatsuki M, Iwagami S, Nosho K, Baba H.
    • Journal Title

      Clin Cancer Res.

      Volume: 19(9) Pages: 2451-9

    • DOI

      10.1158/1078-0432.CCR-12-3559

    • Peer Reviewed
  • [Journal Article] KRAS and BRAF Mutations in 203 Esophageal Squamous Cell Carcinomas : Pyrosequencing Technology and Literature Review2013

    • Author(s)
      Shigaki H, Baba Y, Watanabe M, Miyake K, Murata A, Iwagami S, Ishimoto T, Iwatsuki M, Yoshida N, Baba H.
    • Journal Title

      Ann Surg Oncol

      Volume: 20(3) Pages: 485-91

    • DOI

      10.1245/s10434-012-2819-z

    • Peer Reviewed
  • [Journal Article] Methylation levels of LINE-1 in primary lesion and matched metastatic lesions of colorectal cancer2013

    • Author(s)
      Murata A, Baba Y, Watanabe M, Shigaki H, Miyake K, Ishimoto T, Iwatsuki M, Iwagami S, Sakamoto Y, Miyamoto Y, Yoshida N, Nosho K, Baba H.
    • Journal Title

      Br J Cancer

      Volume: 109(2) Pages: 408-15

    • DOI

      10.1038/bjc.2013.289

    • Peer Reviewed
  • [Presentation] 食道扁平上皮癌におけるAKT pathwayの網羅的解析2013

    • Author(s)
      馬場祥史、渡邊雅之、志垣博信、小澄敬祐、原田和人、村田飛鳥、石本崇胤、岩上志朗、吉田直矢、馬場秀夫
    • Organizer
      第51回日本癌治療学会学術集会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2013-10-24
  • [Presentation] 大腸癌原発巣と遠隔転移巣におけるGenetic and Epigenetic Alterationの網羅的比較2013

    • Author(s)
      村田飛鳥、馬場祥史、渡邊雅之、志垣博信、岩上志朗、坂本快郎、宮本裕士、別府透、馬場秀夫
    • Organizer
      第68回日本消化器外科学会
    • Place of Presentation
      サンホテルフェニックス(宮崎県)
    • Year and Date
      2013-07-17
  • [Presentation] Pyrosequencing technologyを用いて食道癌におけるPIK3CA遺伝子変異の検討2013

    • Author(s)
      志垣博信、馬場祥史、渡邊雅之、村田飛鳥、石本崇胤、岩槻政晃、尾崎宣之、岩上志朗、坂本快郎、宮本裕士、吉田直矢、馬場秀夫
    • Organizer
      第113回日本外科学会定期学術集会
    • Place of Presentation
      マリンメッセ福岡
    • Year and Date
      2013-04-12
  • [Presentation] Relationship between LINE-1 methylation level, allelic imbalance and PIK3CA mutation in esophageal squamous cell carcinoma2013

    • Author(s)
      Baba Y, Watanabe M, Murata A, Shigaki H, Iwagami S, Iwatsuki M, Ishimoto T, Baba H.
    • Organizer
      AACR Annual Meeting 2013
    • Place of Presentation
      Washington Convention Center, Washington, DC
    • Year and Date
      2013-04-09
  • [Presentation] PIK3CA mutation is associated with a favorable prognosis in esophageal squamous cell carcinoma2013

    • Author(s)
      Shigaki H, Baba Y, Watanabe M, Miyake K, Murata A, Ozaki N, Iwagami S, Ishimoto T, Iwatsuki M, Sakamoto Y, Miyamoto Y, Yoshida N, Baba H.
    • Organizer
      9th AACR-Japanese Cancer Association Joint Conference
    • Place of Presentation
      Hyatt Regency Maui, Hawaii
    • Year and Date
      2013-02-22
  • [Presentation] he frequency of BRAF mutation in esophageal squamous cell carcinoma detected by Pyrosequencingパイロシークエンスで検出された食道扁平上皮癌におけるBRAF変異率の検討2012

    • Author(s)
      志垣博信、馬場祥史、渡邊雅之、三宅慧輔、尾崎宣之、岩上志朗、石本崇胤、吉田直矢、馬場秀夫
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      ホテルロイトン札幌(北海道)
    • Year and Date
      20120919-21

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Published: 2015-07-16  

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