2013 Fiscal Year Final Research Report
Fluorescence diagnosis of metastatic lymph nodes in gastric cancer patients using 5-ALA.
| Project/Area Number |
23591944
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
NAKANISHI Masayoshi 京都府立医科大学, 医学(系)研究科(研究院), 講師 (20516190)
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| Co-Investigator(Kenkyū-buntansha) |
OTSUJI Eigo 京都府立医科大学, 医学研究科, 教授 (20244600)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 胃癌 / リンパ節転移 / 蛍光診断 / 5-ALA |
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| Research Abstract |
5-aminolevulinic acid (5-ALA) is a natural amino acid and can be converted into a photosensitizer, protoporphyrin IX (PpIX), via the heme biosynthetic pathway. Gastric cancer cell lines with 5-ALA were positive for PpIX fluorescence. After injection of 5-ALA to mouse model via the tail vein, PpIX fluorescence could be detected on metastatic lymph nodes by stereomicroscopic imaging.
A total of 144 lymph nodes obtained from 14 gastric cancer patients were examined. The patients were administered 5-ALA orally before surgery. The diagnostic results were compared to those of the routine H&E stain examination. Observed PpIX fluorescence of was identical to the metastatic lymph nodes, with 92.4 % accuracy. The area under the curve was calculated as 0.832 throughout Receiver operating characteristic (ROC) analysis.
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